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Castro-Aceituno, Veró,nica,Abbai, Ragavendran,Moon, Seong Soo,Ahn, Sungeun,Mathiyalagan, Ramya,Kim, Yu-Jin,Kim, Yeon-Ju,Yang, Deok Chun EDITIONS SCIENTIFIQUES ELSEVIER 2017 BIOMEDICINE AND PHARMACOTHERAPY Vol.93 No.-
<P><B>Abstract</B></P> <P> <I>Pleuropterus multiflorus</I> (Hasuo) is a widely used medicinal plant in Korea and China for treating amnesia, isnomia, heart throbbing etc. With the constructive idea of promoting the wide-spread usage of <I>P. multiflorus</I>, we propose its indirect usage in the form of biologically active silver (Pm-AgNPs) and gold nanoparticles (Pm-AuNPs). The synthesized nanoparticles were predominantly spherical, crystalline with the Z-average hydrodynamic diameter of 274.8nm and 104.8nm respectively. Also, proteins and phenols were identified as the major players involved in their synthesis and stability. Further, Pm-AgNPs at 25μg/mL were significantly cytotoxic to lung cancer cells, whereas, Pm-AuNPs were not cytotoxic to both normal keratinocyte and lung cancer cells even at 100μg/mL. In addition, further evaluation of the anti-cancer activity of these new nanoparticles, such as migration and apoptosis, shown that Pm-AgNPs have a potential therapeutic effect on A549 lung cancer cell treatment. To the best of our knowledge, this is the first report dissecting out the ability of the endemic <I>P. multiflorus</I> for the synthesis of bioactive silver and gold nanoparticle which would open up doors for its extensive usage in medicinal field.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Veronica, Castro-Aceituno,Siddiqi, Muhammad Hanif,Ahn, Sungeun,Sathishkumar, Natarajan,Noh, Hae Yong,Simu, Shakina Yesmin,Jimenez Perez, Zuly E.,Yang, Deok Chun Current Science Association 2016 Current science Vol.111 No.6
<P>In previous studies compound K (CK), an active metabolite ginsenoside from Panax ginseng, was shown to exhibit anti-cancer activity. However, the mechanism of CK through the EGFR/H-Ras pathway in cancer cells has not been reported so far. Therefore, we focused on the effect of CK as an EGFR and H-Ras inhibitor by in silico and in vitro studies using A549 cells. The biological activity prediction shows that CK exhibits chemopreventive, anticarcinogenic and anti-metastatic activity. Also, using molecular docking studies it has been shown that CK exhibits a strong binding energy with EGFR and H-Ras than Erlotinib. CK inhibits cell viability, decreases cell migration, induces apoptosis and strongly decreases gene expression of EGFR and H-Ras genes in vitro. This finding suggests that the EGFR pathway is involved in the anti-cancer activity of CK of EGF-enhanced A549 lung cancer cell line.</P>
In silico and In vitro Analysis of Anti-lung cancer Activity of Compound K in EGF-induced A549 Cells
Muhammad Hanif Siddiqi,Veronica Castro Aceituno,Sungeun Ahn,Shakina Yesmin Simu,Zuly Elizabeth Jimenez Perez,Deok-Chun Yang 한의병리학회 2015 대한동의병리학회 학술대회논문집 Vol.2015 No.10
Ginseng, the root of Pctnax ginseng (P. ginseng), is an oriental traditional herb used in treatment of several human disorder. Ginsenoside, the active compound of P. ginseng have reported as anti-inflammation, anti-osteoporosis and anti-tumour activity. However, the combined in-silico and in-vitro anti-lung cancer activity of compound K (CK) has not been reported so far. Therefore, the purpose of this study was to evaluate the anti-lung cancer activity of CK using in silico docking study followed by in-vitro validation using human lung cancer A549 cells. In recent study we reported that CK could impede growth, and proliferation as well as induce apoptosis in human lung cancer A549 cells. The in-silico study for CK and H-Ras protein was performed by molecular docking simulation. On the other hand, anti-proliferative, anti-migratory and apoptotic activity of C-K were measured by BrdU assay, wound-healing assay and Hoechst-staining respectively. We also evaluated the inhibitory activity of CK on mRNA expression levels of EGFR, H-Ras and ELK1 by realtime PCR (RT-PCR) analysis. Our molecular docking results showed that compared with a control drug (Erlotinib), CK strongly interact with H-Ras protein with good binding energy. Also, our in-vitro results revealed that C-K had the capability to suppress the proliferation, as well as decrease cell migration in A549 cells. Moreover, CK-induced apoptosis and could be blocked mRNA expression level of aforementioned genes in human lung cancer A549 cells. Taken together, these results indicate that CK exerts antiproliferative effects on A549 cells in-vitro and in-silico via EGFR/H-Ras signals.
Kim, Chun Gon,Castro-Aceituno, Veró,nica,Abbai, Ragavendran,Lee, Hyun A.,Simu, Shakina Yesmin,Han, Yaxi,Hurh, Joon,Kim, Yeon-Ju,Yang, Deok Chun Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.99 No.-
<P><B>Abstract</B></P> <P>Siberian ginseng (<I>Eleutherococcus senticosus</I>) was used for the synthesis of an ecofriendly silver nanoparticle (Sg-AgNP), which has exhibited antibacterial, antioxidant effect and lower cytotoxicity to normal cells in comparison to human cancer cells. Although, the potential anticancer activity of Sg-AgNP has not been determined. In this study, two cancer cell lines were used to evaluate the cytotoxicity and apoptotic effect of Sg-AgNP along with the determination of the role of the Caspase-3 / p38 MAPK pathways. Results shown that Sg-AgNP reduced the cell viability of colon cancer cells HT29 and lung cancer cells A549. The cytotoxic effect was higher than the effect exhibited by a commercial silver nanoparticle and Cisplatin. Reactive oxygen species were observed to be superior in both cell lines in the presence of Sg-AgNPs than c-AgNPs and Cisplatin. It was observed an activation of <I>MAPK14</I> gene and phosphorylation of p38 MAPK protein in both cell lines induced by Sg-AgNPs treatment. Furthermore, induction of morphological changes in the nucleus was done by Sg-AgNPs at 10 μg/mL in both cell lines. On the other hands, the activation of <I>CASP3</I> gene and Caspase-3 protein was observed in HT29 cells but only at protein level in A549 cells. These results, suggest that Sg-AgNPs anticancer potential activity might be linked to the induction of apoptosis though the generation of ROS by activation of the Caspase-3/p38 MAPK pathway.</P>
Singh, Priyanka,Singh, Hina,Castro-Aceituno, Veró,nica,Ahn, Sungeun,Kim, Yeon Ju,Farh, Mohamed El-Agamy,Yang, Deok Chun KLUWER 2017 JOURNAL OF NANOPARTICLE RESEARCH Vol.19 No.7
<P>The current study highlights the fabrication of drug delivery system by utilizing 200 nm mesoporous silica nanoparticles (MSNPs) with 4-nm pore size, as a carrier system for delivery ginsenoside compound K (CK) and Rh2 to enhance their efficacy. The two pharmacologically imperative ginsenosides, CK and Rh2, were loaded to the MSNPs to prepare MSNPs-CK and MSNPs-Rh2, respectively. A fluorescein isothiocyanate (FITC) fluorescent dye was combined in the MSNPs carrier system, in order to trace the cellular uptake of ginsenoside-loaded nanoparticles for in vitro studies. Following purification, the so-prepared MSNPs-CK-FITC and MSNPs-Rh2-FITC were characterized by several analytical techniques, which includes, high-pressure liquid chromatography (HPLC), H-1 NMR, field emission transmission electron microscopy (FE-TEM), Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), thermogravimetric analysis (TGA), and dynamic light scattering (DLS). In vitro cytotoxicity assay in HaCaT skin cells, A549 lung cancer cells, HepG2 liver carcinoma cells, and HT-29 colon cancer cell lines were tested for MSNPs-CK-FITC and MSNPs-Rh2-FITC. The results demonstrate the excellent biocompatibility of nanoparticles in normal cell lines (HaCaT skin cells) and anticancer efficacy in all the tested cancer cell lines at 10-mu M concentration. Additionally, the in vitro anti-inflammatory behavior of MSNPs-CK-FITC and MSNPs-Rh2-FITC were checked in RAW264.7 (murine macrophage) cell lines. The outcomes showed higher anti-inflammatory efficacy of MSNPs-CK-FITC and MSNPs-Rh2-FITC as compared to standard ginsenosides CK and Rh2 in RAW264.7 cell lines. Thus, with 200 nm MSNPs carrier system for the delivery ginsenosides CK and Rh2, a high amount of loading and increasing in vitro pharmacological efficacies of ginsenosides were realized. This study may provide useful insights for designing and improving the applicability of MSNPs for ginsenoside delivery.</P>
Wang, Chao,Mathiyalagan, Ramya,Kim, Yeon Ju,Castro-Aceituno, Veronica,Singh, Priyanka,Ahn, Sungeun,Wang, Dandan,Yang, Deok Chun Dove Medical Press 2016 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.11 No.-
<P><I>Dendropanax morbifera</I> Léveille is an oriental medicinal plant that is traditionally used in folk medicine and grows in a specific region of South Korea. We aimed to enhance the utilization of <I>D. morbifera</I> medicinal plants for synthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs). <I>D. morbifera</I> leaf extract acted as both a reducing and a stabilizing agent that rapidly synthesized <I>Dendropanax</I> AgNPs (D-AgNPs) and <I>Dendropanax</I> AuNPs (D-AuNPs). The D-AgNPs and D-AuNPs were characterized by ultraviolet-visible spectroscopy, energy dispersive X-ray analysis, elemental mapping, field emission transmission electron microscopy, X-ray diffraction, and dynamic light scattering. The characterizations revealed that the D-AgNPs and D-AuNPs were in polygon and hexagon shapes with average sizes of 100–150 nm and 10–20 nm, respectively. The important outcomes were the synthesis of AgNPs and AuNPs within 1 hour and 3 minutes, respectively, avoiding the subsequent processing for removal of any toxic components or for stabilizing the nanoparticles. Additionally, D-AgNPs and D-AuNPs were examined for cytotoxicity in a human keratinocyte cell line and in A549 human lung cancer cell line. The results indicated that D-AgNPs exhibited less cytotoxicity in the human keratinocyte cell line at 100 µg/mL after 48 hours. On the other hand, D-AgNPs showed potent cytotoxicity in the lung cancer cells at the same concentration after 48 hours, whereas D-AuNPs did not exhibit cytotoxicity in both cell lines at the same concentration. However, both D-AgNPs and D-AuNPs at 50 µg/mL enhanced the cytotoxicity of ginsenoside compound K at 25 µM after 48 hours of treatment compared with CK alone. We believe that this rapid green synthesis of D-AgNPs and D-AuNPs is a valuable addition to the applications of <I>D. morbifera</I> medicinal plant. D-AuNPs can be used as carriers for drug delivery and in cancer therapy due to their lack of normal cell cytotoxicity.</P>