에이전트 기반 Mobile IP 라우팅 최적화에 관한 연구

김보균 제주한라대학 2002 論文集 Vol.26 No.-

With recent advances in wireless communication technology, mobile computing is an increasingly important area of research. Enabling mobility in IP networks is a significant issue for making use of many portable devices appearing on the Internet. The IP mobility support being standardized in the IETF utilizes tunneling of IP packets from a Home Agent to a Foreign Agent to make the mobility transparent to the higher layer. In this paper, we propose an approach to optimize routing path and avoid triangular routing problem in IP mobility, which a routing table, called Mobile Routing Table(MRT), is designed in each edge router such as Home Agent, Foreign Agent and general router. A packet retransmission scheme is also proposed to reduce the packet loss during handoff. We analyze and compare both the standard Mobile IP and the proposed seamless handoff approach. Finally, the simulation results are presented.

Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii)

Ju Lan Kim,Hee Jeong Kong,Hyung Soo Kim,Woo-Jin Kim,Dong-Gyun Kim,Bo-Hye Nam,Young-Ok Kim,Cheul Min An 한국발생생물학회 2014 발생과 생식 Vol.18 No.4

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

Leptomycin B Increases Radiosensitization by Trichostain A in HeLa Cells

In Ah Kim(김인아),Jin Ho Kim(김진호),Jin Hee Shin(신진희),Il Han Kim(김일한),Jae Sung Kim(김재성),Hong Gyun Wu(우홍균),Eui Kyu Chie(지의규),Yong Ho Kim(김용호),Bo Kyung Kim(김보경),Semie Hong(홍세미),Sung Whan Ha(하성환),Chan Il Park 대한방사선종양학회 2005 Radiation Oncology Journal Vol.23 No.2

목 적: 히스톤탈아세틸화효소 억제제는 그 자체의 항암효과뿐만 아니라 방사선 감작제로서의 효과가 점차 분명해져가고 있다. 최근 Class I 특이적인 히스톤탈아세틸화효소 억제제의 개발로 계층 특이적인(class specific) 연구가 가능해짐에 따라, 본 연구에서는 서로 다른 히스톤탈아세틸화효소억제제의 방사선감작효과를 비교함과 동시에 p53 발현도의 차이가 히스톤탈아세틸화효소억제제의 방사선 감수성에 미치는 영향을 알아보고자 하였다. 대상 및 방법: 이를 위해 p53 발현도가 매우 낮은 HeLa 세포에 p53의 핵 외 수송을 억제하여 세포질 내 분해를 차단하는 Leptomycin B를 처리하여 p53의 발현도를 현저하게 높인 후, Trichostatin와 SK7041의 방사선 민감도를 비교 관찰하였다. 결 과: 세포생존곡선, SER 및 SF2를 비교 분석 시, p53의 발현이 높은 Leptomycin B 처리군에서 TrichostatinA가 Class I HDAC만을 억제하는 SK7041에 비해 유의하게 높은 방사선 감작효과를 나타내었다. 이는 p53이 Class I 특이적 억제제인 SK7041과 Class I과 II를 모두 억제하는 TSA의 방사선감작효과에 미치는 영향의 차이에 기전적으로 관여함을 시사한다. 결 론: Leptomycin B에 의해 유도된 p53의 발현증가는 Class I과 Class I과 II를 모두 억제하는 TSA의 방사선 감작효과를 증강시킨다. Purpose: Histone deacetylase inhibitors (HDIs) are emerging as potentially useful components of anticancer therapy and their radiosensitizing effects have become evident. Specific HDIs are now available that preferentially inhibit specific HDAC classes; TSA inhibits Class I and II HDACs, and SK7041 inhibits Class I HDACs. Materials and Methods: We tested the differential radiosensitization induced by two different classes of HDIs in HeLa cells. We next tested the hypothesis that p53 expression in cancer cells may influence the susceptibility to HDIs by using pharmacologic modification of the p53 status under an isogenic background. Results: It is interesting that p53 expression in the HeLa cells clearly increased the degree of radiosensitization by TSA compared to that of the class I specific inhibitor SK7041. This suggests that p53 may, in part, be responsible for the mechanistic role for the greater radiosensitization induced by Class I & II inhibitors compared to that of the class I specific inhibitors. Thus, these studies are useful in distinguishing between events mediated solely by the Class I HDACs versus those events involving the other classes of HDACs as well. Conclusion: The anticancer efficacy of targeting Class I and II HDACs, in conjunction with radiation therapy, may be further enhanced by the restoration of p53 expression.

Vitellibacter todarodis sp. nov., isolated from intestinal tract of a squid (Todarodes pacificus)

Kim, Hyun Chul,Kim, Young-Ok,Park, Sooyeon,Nam, Bo-Hye,Kim, Dong-Gyun,Park, Ji-Min,Yoon, Jung-Hoon Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.4

Genetic Organization of Two Types of Flounder Warm-Temperature Acclimation-Associated 65-kDa Protein and Their Gene Expression Profiles

KIM, Young-Ok,PARK, Eun-Mi,MOON, Ji Young,NAM, Bo-Hye,KIM, Dong-Gyun,KONG, Hee Jeong,KIM, Woo-Jin,JEE, Young-Ju,LEE, Sang-Jun Japan Society for Bioscience, Biotechnology and Ag 2013 Bioscience, biotechnology, and biochemistry Vol.77 No.10

Association of Acute Myocardial Infarction with Ossification of the Posterior Longitudinal Ligament in Korea: A Nationwide Longitudinal Cohort Study

Kim Jaehwan,Kim Chai Yoon,Kim Jeong Gyun,Kim Hakyung,Sheen Seung Hun,Han In-bo,Sohn Seil 대한말초신경학회 2023 The Nerve Vol.9 No.1

Objective: This nationally matched longitudinal study aimed to investigate the relationship between acute myocardial infarction (AMI) and ossification of the posterior longitudinal ligament (OPLL) in Korea.Methods: We collected patient data from January 1, 2004 to December 31, 2015 from the National Health Insurance Service Health Screening Cohort. Patients with OPLL were defined as patients with the International Classification of Diseases, Tenth Revision code M48.8 (other specified spondylopathies) and were newly diagnosed through computed tomography imaging. The OPLL group had a total of 1,289 patients. The control group included 6,445 people. Utilizing the Kaplan-Meier technique, The incidence of AMI in both groups was estimated. A Cox proportional-hazards regression analysis was used to compute the AMI hazard ratio.Results: After controlling for age and sex, the hazard ratio of AMI in the OPLL group was 2.065 (95% confidence interval [CI], 1.228-3.474). The adjusted hazard ratio in the OPLL group was 2.209 after restricting the sample for demographics and concomitant medical conditions (95% CI, 1.311-3.721). In a subgroup analysis, the incidence of AMI was substantially greater in the OPLL group, which included women younger than 65 years and without hypertension, diabetes, or dyslipidemia.Conclusion: This nationwide longitudinal study found that patients with OPLL were at higher risk of AMI.

Gene Cloning and Characterization of a Cold-Adapted Esterase from Acinetobacter venetianus V28

( Kim Young Ok ),( Yu Li Heo ),( Hyung Kwoun Kim ),( Bo Hye Nam ),( Hee Jeong Kong ),( Dong Gyun Kim ),( Woo Jin Kim ),( Bong Seok Kim ),( Young Ju Jee ),( Sang Jun Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9

Acinetobacter venetians V28 was isolated from the intestine of righteye flounder, Poecilopsetta plinthus caught in Vietnam seawater, and the esterase gene was cloned using a shotgun method, The amino acid sequence deduced from the nucleotide sequence (1,017bp) corresponded to a protein of 338 amino acid residues with a molecular weight of 37,186. The esterase had 87% and 72% identities with the lipases of A. junii SH205 and A. calcoaceticus RUH2202, respectively. The esterase contained a putative leader sequence, as well as the conserved catalytic triad (Ser, His, Asp), consensus pentapeptide GXSXG, and oxyanion hole sequence (HG). The protein from the strain V28 was produced in both a soluble and an insoluble form when the Escherichia coli cells harboring the gene were cultured at 18oC. The maximal activity of the purified enzyme was observed at a temperature of 40oC and pH 9.0 using p-NP-caprylate as substrate; however, relative activity still reached to 70% even at 5oC with an activation energy of 3.36 kcal/mol, which indicated that it was a cold-adapted enzyme. The enzyme was a nonmetalloprotein and was active against p-nitrophenyl esters of C4, C8, and C14. Remarkably, this enzyme retained much of its activity in the presence of commercial detergents and organic solvents. This cold-adapted esterase will be applicable as catalysts for reaction in the presence of organic solvents and detergents.

Shewanella sp. Ke75 Esterase with Specificity for p-nitorphenyl Butyrate: Gene Cloning and Characterization

Kim, Young-Ok,Park, In-Suk,Kim, Hyung-Kwoun,Nam, Bo-Hye,Kong, Hee Jeong,Kim, Woo-Jin,Kim, Dong-Gyun,Kim, Bong-Seok,Jee, Young-Ju,Song, Jung-Hun,Lee, Sang-Jun The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1

A bacterial strain that produces a cold-adapted esterase was isolated from tidal flats and identified as Shewanella sp. Ke75. In the present study, the corresponding gene was cloned using the shotgun method. The amino acid sequence deduced from the nucleotide sequence (957 bp) corresponded to a protein of 318 amino acid residues with a calculated molecular weight of 34875 Da. The esterase showed 68 and 57% identities with the putative esterases of Shewanella amazonensis SB2B and Colwellia psychrerythraea 34H, respectively. The esterase contained a putative leader sequence, as well as the conserved catalytic triad (Ser, His, Asp), consensus pentapeptide GXSXG, and oxyanion hole sequence (HG). The protein Ke75 was produced in both soluble and insoluble forms when Escherichia coli cells harboring the gene were cultured at $30^{\circ}C$. The enzyme showed specificity for C4 (butyrate) as a substrate, with little activity toward the other p-nitrophenyl esters tested. The optimum pH and temperature for enzyme activity were pH 9.0 and $30^{\circ}C$, respectively. Relative activity remained up to 60% even at $5^{\circ}C$ with an activation energy of 6.29 kcal/mol, which indicated that it was a cold-adapted enzyme. Enzyme activity was enhanced in the presence of $Mn^{2+}$ ions, but inhibited by $Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, and $Zn^{2+}$ ions.

Molecular characterization of <i>Rhodeus uyekii</i> tripartite motif protein 1 (TRIM1) involved in IFN-γ/LPS-induced NF-κB signaling

Kim, Julan,Kim, Ju-Won,Kim, Dong-Gyun,Nam, Bo-Hye,Kim, Young-Ok,Park, Jung Youn,Kong, Hee Jeong Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.79 No.-

<P><B>Abstract</B></P> <P>The tripartite motif-containing (TRIM) proteins are involved in a wide range of cellular processes, and the role of TRIM1 in immunity has been explored. However, fundamental studies on fish TRIM1 are lacking. In this study, we cloned and characterized TRIM1 cDNA from the Korean rose bitterling, <I>Rhodeus uyekii</I> (RuTRIM1). Two RuTRIM1 isoforms (RuTRIM1-X1 and RuTRIM1-X2) were identified. The coding sequence (CDS) of RuTRIM1-X1 comprised 2157 bp encoding a 718-aa protein, and the CDS of RuTRIM1-X2 comprised 1929 bp encoding a 642-aa protein. Both RuTRIM1 isoforms contained a RING finger domain, B-box 1, B-box 2, coiled-coil domain, COS box, FN3 motif, and PRY/SPRY domain. The deduced RuTRIM1-X1 and RuTRIM1-X2 proteins had high amino acid identity (76.27–98.89%) with orthologs from various other species, and a phylogenetic tree was constructed. RuTRIM1-X1 and RuTRIM1-X2 mRNA were expressed in all tissues examined, with the highest expression levels detected in the hepatopancreas. During early development, RuTRIM1-X1 and RuTRIM1-X2 mRNA levels changed differently from the gastrula period to the first feeding stage. An <I>in vivo</I> ubiquitination assay showed that RuTRIM1 exhibited RING-dependent E3 ubiquitin ligase activity, mainly by comparing RuTRIM1-X2 to RuTRIM1-X1. The subcellular localization of the two RuTRIM1 protein isoforms was characterized, revealing that they formed aggregates in cytoplasmic bodies in Raw264.7 cells. Interferon-γ/lipopolysaccharide-induced nuclear factor-κB signaling was negatively regulated by RuTRIM1-X1 and RuTRIM1-X2, and the negative effect was reversed in RING deletion mutants. To our knowledge, this is the first study to characterize fish TRIM1, which may play a role in the inflammatory response.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We isolated two TRIM1 isoforms (RuTRIM1-X1 and RuTRIM1-X2) from <I>Rhodeus uyekii.</I> </LI> <LI> RuTRIM1 cDNAs encode polypeptides with the RBCC motif. </LI> <LI> RuTRIM1 proteins share 76.27–98.89% homology with orthologs from other species. </LI> <LI> The subcellular localization and Ub ligase activity of the two RuTRIM1 protein isoforms were characterized. </LI> <LI> RuTRIM1-X1 and RuTRIM1-X2 negatively regulate the IFN-γ/LPS-induced NF-κB signaling. </LI> </UL> </P>

Identification and Characterization of a Bacteriocin Produced by an Isolated Bacillus sp. SW1-1 that Exhibits Antibacterial Activity against Fish Pathogens

Kim, Young-Ok,Park, In-Suk,Kim, Dae-Jung,Nam, Bo-Hye,Kim, Dong-Gyun,Jee, Young-Ju,An, Cheul-Min The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5

The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at $30^{\circ}C$ after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at $100^{\circ}C$ for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0-11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.