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        Genome wide survey and analysis of microsatellites in Tombusviridae family

        Biju George,Binu George,Mayur awasthi,Ram Nageena Singh 한국유전학회 2015 Genes & Genomics Vol.37 No.8

        Microsatellites are small repetitive sequences found in all kingdoms of life including viruses. Members of the Tombusviridae family severely affect growth of wide range of plants and reduce economic yield in diverse cropping systems worldwide. Here, we analyzed the nature and distribution of both simple and complex microsatellites present in complete genome of 47 species of Tombusviridae family. Our results showed, in all analyzed genomes, genome size and GC content had a weak influence on number, relative abundance and relative density of microsatellites, respectively. For each genome, dinucleotide repeats followed by mononucleotide repeats were found to be highly predominant. Mononucleotide repeats were found to be underrepresented in majority of Tombusviridae genomes. Poly G and Poly C repeats were more abundant as compared to poly A or poly T nucleotides. TG/GT and AG/CA was the most abundant dinucleotide repeat motif observed in Tombusviridae genomes. Repeats larger than trinucleotide were never found in these viral genomes. Comparative study of relative abundance and density of microsatellite among available RNA and DNA viral genomes indicated that simple repeats were lower than many RNA virus genomes. To our knowledge, this is the first analysis of microsatellites occurring in Tombusviridae genomes. Characterization of such variations in repeat sequences would be important in deciphering the origin, mutational processes, and role of repeat sequences in viral genomes.

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        Electronic structure and luminescence characteristics of rare earth free self-activated Ca2Sb2O7 blue emitting phosphor

        George Anns,Mohan P Remya,Aiswarya P.A.,Rajalakshmi C.,Ipe Thomas Vibin,Joseph Cyriac,Biju P.R. 한국물리학회 2022 Current Applied Physics Vol.39 No.-

        Over the past few years, extensive research has been focused on the development of rare-earth-free phosphors to achieve energy-efficient and cost-effective WLEDs with high luminous efficiencies for solid-state lighting applications. Herein, we report a novel rare-earth-free self-activated blue phosphor Ca2Sb2O7, synthesized by solidstate reaction method. The electronic structure and bandgap of the material were determined by the density functional theory (DFT + U) method using Perdew-Burke-Ernzerhof (PBE) exchange-correlation functional. The prepared phosphor could be effectively excited in the range 280–370 nm resulting in a broad blue emission band, owing to the Sb5+ → O2- transition in the (SbO6)7- group. The CIE chromaticity coordinates were found to be (0.144, 0.097) indicating intense blue emission with high color purity of 89%. Also, the prepared phosphor has good thermal stability and an internal quantum efficiency of 24%. All these results indicate that self-activated Ca2Sb2O7 could be a potential blue phosphor for lighting and display devices.

      • Seasonal and inter-annual variability of soil moisture stress function in dryland wheat field, Australia

        Akuraju, Venkata Radha,Ryu, Dongryeol,George, Biju,Ryu, Youngryel,Dassanayake, Kithsiri Elsevier 2017 Agricultural and forest meteorology Vol.232 No.-

        <P><B>Abstract</B></P> <P>It is assumed that the ratio of actual evapotranspiration (AET) to potential evapotranspiration (PET) is mostly controlled by the soil water content available for ET. This control is formulated using the soil moisture stress function (SSF), where the evaporative fraction (EF) or the fraction of the AET to PET (fPET) is assumed to be either a linear or a non-linear function of soil moisture. We examine the effectiveness of the soil moisture stress function to quantify soil moisture control on EF or fPET over a dryland wheat field in Victoria, Australia. Micrometeorological observations from two cropping seasons were used for the analysis. The efficacy of a root-density-weighted soil moisture estimate in predicting EF and fPET was investigated as against the commonly assumed fixed-depth root zone soil moisture. However, results indicate a strong relationship between EF and available soil water fraction (AWF) in the root zone only when solar radiation is higher than 5MJ/m<SUP>2</SUP>/day. As the rooting depth increases with vegetation growth, SSF exhibits the strongest correlation with AWF for increasing soil profile depth. In the early and harvesting crop growth stages, ET is constrained mostly by surface soil moisture (0–5cm). In the mid-growth stages, ET is strongly influenced by soil moisture in the root zone (0–60cm). The shape of SSF, however, changes significantly between the two years (2012 and 2013). It is inferred that different temporal rainfall patterns between the years caused wheat’s different response to water stress.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Net radiation and crop phenology affect soil moisture stress function (SSF). </LI> <LI> Adaptive definition of root depth is important for accurate SSF. </LI> <LI> Root density profile needs to be used when defining root zone soil moisture. </LI> <LI> SSF can change inter-annually with temporal pattern of soil moisture. </LI> </UL> </P>

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