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        Non-thermal plasma enhances rice seed germination, seedling development, and root growth under low-temperature stress

        Bian Jing-Yang,Guo Xiao-Yu,Lee Dong Hun,Sun Xing-Rong,Liu Lin-Shuai,Shao Kai,Liu Kai,Sun Hu-Nan,Kwon Taeho 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

        Recently, non-thermal plasma (NTP) technologies have found widespread application across diverse fields, including plant growth, medical science, and biological and environmental research. Rice (Oryza sativa L.) is exceptionally sensitive to temperature changes. Notably, low-temperature stress primarily affects the germination and reproductive stages of rice, often leading to reduced crop yield. This study aimed to identify optimal conditions for enhancing rice seed germination and seedling growth under low temperatures using NTP technology. Our research indicated that NTP treatment at 15.0 kV for 30 s optimally promotes rice seed germination and growth under low-temperature stress. Furthermore, NTP treatment increases the activity and expression of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), under low-temperature conditions. Moreover, it downregulates the expression of β-ketoacyl-[acyl carrier protein] synthase I (KASI) and cis-epoxy carotenoid dioxygenase 3 (NCED3) and upregulates the expression of alternative oxidase (AOX1B), BREVIS RADIX-like homologous gene (BRXL2), WRKY transcription factor 29 (WRKY29), and EREBP transcription factor 2 (EREBP2) in roots after tandem 7 days lowtemperature (16 ℃) and 7 days room-temperature (28 ℃) treatments. Transcriptomic analysis revealed the involvement of various key genes in phosphotransferase activity, phosphate-containing compound metabolic processes, and defense responses. These analyses provide comprehensive information on gene expression at the transcriptional level, offering new insights for a deeper understanding of candidate genes required for root growth in rice.

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        Monitoring Analysis and Numerical Simulation of the Land Subsidence in Linear Engineering Areas

        Chao Jia,Xiao Yang,Jing Wu,Pengpeng Ding,Chao Bian 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7

        Non-uniform land subsidence can cause subgrade deformation and threaten the safety of linear projects such as high-speed railways. The Lunan high-speed railway is taken as the research background. Combined with the hydrogeological and engineering geological conditions, the regional land subsidence is analyzed by the combined method of the differential interferometric synthetic aperture radar (D-InSAR) and small baseline subset interferometric synthetic aperture radar (SBAS-InSAR), which is verified by using precise leveling and GPS monitoring data. The accurate land subsidence analysis of large-scale linear engineering is realized. The distribution situation and change laws of land subsidence in linear engineering areas are revealed. Then, a three-dimensional coupled numerical model of land subsidence along a large-scale linear project is established by using the finite difference method. The spatial distribution characteristics of land subsidence in the linear engineering area are quantitatively analyzed and predicted. The main factors affecting land subsidence and the degree of land subsidence are discussed while keeping the pumping rate constant. The results show that: 1) The combined method of D-InSAR and SBAS-InSAR has high accuracy in the land subsidence monitoring of linear engineering. 2) There are five non-uniform settlement funnels along the Lunan high-speed railway. The groundwater over-extraction and coal mining are the main reasons for the land subsidence. 3) Realizing water source conversion and reduce groundwater exploitation are the main factors in linear engineering areas as soon as possible.

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