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Bhagobaty, Ranjan K.,Purohit, S.,Nihalani, M.C. Korean Society of Microscopy 2015 Applied microscopy Vol.45 No.4
Microorganisms specifically groups of bacteria exhibiting physiological activities of production of acids are a major cause of concern because of their ability to induce corrosion in oil field pipelines and metal systems involved in water handling. Water Injection Stations as a means of secondary recovery from existing oil producing reservoirs, are often employed in most upstream oil and gas industries to ensure replenishment of voidage, maintenance of reservoir pressure and optimization of crude emulsion throughput. In the present study, scanning electron microscopy of macroscopic orange coloured slime formations sampled from leaking valves on the flow-lines of a Water Injection Stations of Oil India Limited revealed the presence of filamentous bacterial mats in association with diatoms. The species composition of the acidic slime formations from the sampled locations reveal the possible role of acid producing iron oxidizing bacteria (IOB) like Acidithiobacillus ferrooxidans in association with Gomphonema sp. in creating conditions for bio-corrosion.
R.K. Bhagobaty,S. R. Joshi 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
Fungal species that establish an endophytic role inside the tissues of medicinal plants are known to produce a wide range of biologically active metabolites and enzymes. In the present study, the most dominant and representative endophytic fungal species of five ethno-medicinal plants prevalent in the pristine sacred forests of Meghalaya, were screened for their ability to produce amylase, cellulase,protease, lipase, and xylanase. Each of endophytic fungal isolates showed a wide range of enzyme activity. Mycelial biomass generation and root colonization, in addition to the enzyme activity of the endophytic fungal isolates, provided insights into their probable origin and ecological roles within the plant host. Fungal species that establish an endophytic role inside the tissues of medicinal plants are known to produce a wide range of biologically active metabolites and enzymes. In the present study, the most dominant and representative endophytic fungal species of five ethno-medicinal plants prevalent in the pristine sacred forests of Meghalaya, were screened for their ability to produce amylase, cellulase,protease, lipase, and xylanase. Each of endophytic fungal isolates showed a wide range of enzyme activity. Mycelial biomass generation and root colonization, in addition to the enzyme activity of the endophytic fungal isolates, provided insights into their probable origin and ecological roles within the plant host.
( Ranjan Kumar Bhagobaty ),( S. R. Joshi ) 한국균학회 2011 Mycobiology Vol.39 No.2
The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial β-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.
R.K. Bhagobaty,S.R. Joshi 한국산림과학회 2011 Forest Science And Technology Vol.7 No.4
Plants growing in unique habitats and having ethno-medicinal usage among the local ethnic populations of an area are often likely to harbour in them novel endophytic fungi. In the present study five ethno-medicinal plants of the traditionally preserved ‘Sacred forests’ of Meghalaya, India were studied for the presence of fungal endophytes in their roots and stems. Isolation in water agar as per standard procedures and subsequent pure culturing of the fungi in potato dextrose agar medium revealed the presence of a variety of endophytic fungal species. A total of 703 fungal isolates were obtained and classified into 16 taxonomic groups based upon their morphological characteristics. The highest diversity of endophytic fungal taxa was observed for Camellia caduca followed by Schima khasiana, Osbeckia chinensis, Potentilla fulgens and Osbeckia stellata.