http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
정시연,장민선,최상호,오세량,우홍화,Yan Zhu,Xiumei Gao,Xiaoying Wang,Baojin Zhang,임다솔,이준영,김소담,송윤선 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.5
Phytoestrogen (PE) has received considerableattention due to the physiological significance of itsestrogenicity. Flemingia strobilifera (FS) has been used asa folk medicine in Asia for the treatment of inflammation,cancer, and infection; however, the estrogenic effects andchemical components of FS have not yet been reported. Weaimed to uncover the estrogenic properties and PEs derivedfrom FS using phytochemical and pharmacological evaluation. PEs from FS extract (FSE) were analyzed by NMR,HPLC, and MS. To evaluate estrogenic activity, FSE andits compounds were evaluated by in vitro and in vivoassays, including human estrogen receptor alpha (hERa)binding, estrogen response element (ERE)-luciferasereporter assays, and uterotrophic assays. FSE and itscompounds 1–5 showed binding affinities for hERa andactivated ERE transcription in MCF-7 cells. Additionally,FSE and compounds 1–5 induced MCF-7 cell proliferationand trefoil factor 1 (pS2) expression. In immature femalerats, significant increases in uterine weight and pS2 genewere observed in FSE-treated groups. We identifiedestrogenic activities of FSE and its bioactive compounds,suggesting their possible roles as PEs via ERs. PEs derivedfrom FSE are promising candidates for ER-targeted therapyfor post-menopausal symptoms.