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Balusamy, Sri Renukadevi,Perumalsamy, Haribalan,Huq, Md. Amdad,Balasubramanian, Balamuralikrishnan Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.108 No.-
<P><B>Abstract</B></P> <P> <I>Origanum vulgare</I> commonly known as oregano belongs to mint family (<I>Laminaceae</I>), and native to temperate western and mediterranean region. In our present study, we have identified the bio-active principles of oregano essential oil (EO) and evaluated its apoptotic effects against human stomach cancer cell lines (AGS). The EO altered colony forming characteristics of cancer cell, migration ability of cancer cell and thus prevented cancer cell proliferation. Furthermore, to evaluate the molecular mechanism involved in cancer cell death, we studied the genes that are involved in fatty acid and cholesterol biosynthesis pathway including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (<I>HMGCR)</I>, Acetyl CoA synthase <I>(ACC)</I>, sterol regulatory element-binding protein (<I>SPREPB1)</I> and fatty acid synthase (<I>FASN)</I> respectively. The pathway genes such as <I>HMGCR</I>, <I>ACC</I>, <I>SPREPB1</I> and <I>FASN</I> decreased in transcript profile and protein accumulation resulting in the inhibition of cell growth. The increase of <I>BAX</I> expression and downregulation of <I>BCL2</I> expression indicated that oregano EO induced mitochondrial mediated apoptosis. In addition, the cancer cell undergone apoptosis was also confirmed by Hoechst, PI staining and DNA fragmentation. The present study finds rationale to use the oregano EO for stomach cancer treatments in traditional medicinal practice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Oregano essential oil (EO) exhibits anti-proliferative activity in stomach cancer. </LI> <LI> Oregano EO inhibited metastasis <I>in vitro</I>. </LI> <LI> Migration of cancer cells were inhibited by oregano EO. </LI> <LI> Increase of BAX and downregulation of BCL2 was noticed under oregano EO treatment. </LI> <LI> Fatty acid pathway genes and proteins were downregulated. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Balusamy, S. R.,Rahimi, S.,Cho, Y. G.,Senthil, K.,Yang, D. C. Springer Science + Business Media 2017 Plant growth regulation Vol.81 No.2
<P>Geraniol, the precursor of terpenoid indole alkaloids can be converted to the 10-hydroxy geraniol by the function of geraniol 10-hydroxylase. In our study, for the first time, a full-length cytochrome P450 monooxygenase (P450) geraniol 10-hydroxylase (PgCYP76C9) cDNA was isolated and characterized from Panax ginseng Meyer. The gene has an open reading frame (ORF) of 1503 base pairs and encodes a precursor protein of 501 amino acids residues. The calculated molecular mass of the protein is approximately 56.3 kDa with a predicated isoelectric point of 8.45. Amino acid identities between PgG10H and other P450s of the CYP76 family in the database had revealed that the deduced amino acid of PgG10H sharing a higher sequence homology with geraniol 10-hydroxylase-like proteins encoded by Cinchona calisaya and Lonicera japonica. We implemented a molecular modeling method to evaluate the possible interaction of geraniol with PgG10H active site. Our finding showed that the geraniol was the potential ligand for PgG10H in P. ginseng. Expression of PgG10H gene was tissue-regulated and showed high expression in 3-year-old ginseng flowers and roots. Expression of PgG10H was differentially induced in ginseng, not only during Pseudomonas syringae infection and wounding but also after exposure to methyl jasmonate and salt stress. Furthermore, overexpression of the newly identified ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis caused terpenoid indole alkaloid dihydrositsirikine production and also conferred enhanced resistance to P. syringae.</P>
Balusamy, T.,Sankara Narayanan, T. S. N.,Ravichandran, K.,Lee, Min Ho,Nishimura, T. The Electrochemical Society 2015 Journal of the Electrochemical Society Vol.162 No.6
<P>The present paper addresses the influence of surface mechanical attrition treatment on the characteristic properties of EN8 steel and, to analyze the correlation between the change in properties of the treated steels with their corrosion behavior in 0.6 M NaCl and borate buffer, evaluated by potentiodynamic polarization and electrochemical impedance spectroscopy studies. Surface mechanical attrition treatment of EN8 steel induced plastic deformation, enabled surface nanocrystallization, increased the surface roughness, microstrain and defects/dislocations and, imparted compressive residual stresses. The change in properties of EN8 steel following treatment has caused a deleterious influence on its corrosion resistance in 0.6 M NaCl but an improvement in corrosion behavior in borate buffer. The possible reasons for the change in corrosion behavior of the treated EN8 steel are suggested. Pictorial models are proposed to explain the evolution of surface roughness and the mechanism of corrosion attack of the treated EN8 steels in 0.6 M NaCl and borate buffer.</P>
Thangaraj, Balusamy,T. S. Nellaiappan, Sankara Narayanan,Kulandaivelu, Ravichandran,Lee, Min Ho,Nishimura, Toshiyasu American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.32
<P>The study addresses how surface nanostructuring of AISI 304 stainless steel (SS) by surface mechanical attrition treatment (SMAT) influences its characteristic properties and corrosion behavior in Ringers solution. SMAT of 304 SS induced plastic deformation, enabled surface nanocrystallization, refined the grain size, transformed the austenite phase to strain induced alpha'-martensite phase, increased the surface roughness, induced defects/dislocations, imparted compressive residual stresses at the surface, decreased the contact angle, and increased surface energy. The change in properties of 304 SS following treatment using 5 and 8 mm empty set balls for 15, 30, 45, and 60 min has caused a deleterious influence on its corrosion resistance in Ringers solution, while an improvement in corrosion behavior is observed for those treated using 2 mm ? balls. The increase in surface roughness, transformation of the austenite to a'-martensite phase, a higher extent of deformation, and the presence of larger number of defects/dislocations are main factors responsible for the lower corrosion resistance observed for 304 SS treated using 5 and 8 mm empty set balls in Ringers solution. In spite of having these attributes with a relatively lower extent, 304 SS treated using 2 mm empty set balls offered a better corrosion resistance and exhibits a better passivity. For those treated using 2 mm empty set balls, the ability of the nanocrystalline surface to promote passivation outweighs the deleterious influences caused by the limited amount of deformation and defects/dislocations. Based on the findings of this study, it is recommend that SMAT of 304 SS using 2 mm empty set balls for 1530 min is the optimum condition to achieve the suitable surface profile, surface characteristics with better corrosion resistance.</P>
Devi, Balusamy Sri Renuka,Kim, Yu-Jin,Sathiyamoorthy, Subramaniyum,Khorolragchaa, Altanzul,Gayathri, Sathiyaraj,Parvin, Shohana,Yang, Dong-Uk,Selvi, Senthil Kalai,Lee, Ok Ran,Lee, Sungyoung,Yang, Deok Consultants Bureau [etc.] 2011 Biochemistry Vol.76 No.12
<P>In plants heme containing cytochrome P450 (P450) is a superfamily of monooxygenases that catalyze the addition of one oxygen atom from O2 into a substrate, with a substantial reduction of the other atom to water. The function of P450 families is attributed to chemical defense mechanism under terrestrial environmental conditions; several are involved in secondary and hormone metabolism. However, the evolutionary relationships of P450 genes in Panax ginseng remain largely unknown. In the present study, data mining methods were implemented and 116 novel putative P450 genes were identified from Expressed Sequence Tags (ESTs) of a ginseng database. These genes were classified into four clans and 22 families by sequence similarity conducted at amino acid level. The representative putative P450 sequences of P. ginseng and known P450 family from other plants were used to construct a phylogenetic tree. By comparing with other genomes, we found that most of the P450 genes from P. ginseng can be found in other dicot species. Depending on P450 family functions, seven P450 genes were selected, and for that organ specific expression, abiotic, and biotic studies were performed by quantitative reverse transcriptase-polymerase chain reaction. Different genes were found to be expressed differently in different organs. Biotic stress and abiotic stress transcript level was regulated diversely, and upregulation of P450 genes indicated the involvement of certain genes under stress conditions. The upregulation of the P450 genes under methyl jasmonate and fungal stress justifies the involvement of specific genes in secondary metabolite biosynthesis. Our results provide a foundation for further elucidating the actual function and role of P450 involved in various biochemical pathways in P. ginseng.</P>
Lee, Ok Ran,Kim, Yu-Jin,Devi Balusamy, Sri Renuka,Kim, Min-Kyeoung,Sathiyamoorthy, Subramaniyam,Yang, Deok-Chun Elsevier 2011 Physiological and molecular plant pathology Vol.76 No.2
<P><B>Abstract</B></P><P>Genomic DNA sequence of a <I>γ-thionin</I> in the <I>Panax ginseng</I> plant, termed <I>Pgγ-thionin</I>, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of <I>Pgγ</I>-thionin was ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. <I>Pgγ-thionin</I> was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-<I>Pgγ</I>-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that <I>Pgγ</I>-thionin might be involved in cellular defense responses.</P> <P><B>Graphical abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► We found the genomic DNA of γ-thionins from <I>P. ginseng</I> encodes 225 bp ORF. ► The 3-D structure of Pgγ-thionin is similar to the previously reported γ-thionin. ► <I>Pgγ-thionin</I> was responsive to sets of biotic and abiotic stresses. ► Pgγ-thionin was found to be localized to cell wall-bound extracellular spaces.</P>