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PLGA/DBP 하이브리드지지체와 섬유륜세포 및 수핵세포를 이용한 바이오디스크의 재생
임지예 ( Ji Ye Lim ),김순희 ( Soon Hee Kim ),박상욱 ( Sang Wook Park ),소정원 ( Jeong Won So ),백미옥 ( Mi Ock Back ),김문석 ( Moon Suk Kim ),강길선 ( Gilson Khang ),이종문 ( John M. Rhee ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.1
We fabricated poly(lactide-co-glycolide)(PLGA) scaffolds impregnated 0, 10, 20, 40, and 80 wt% demieneralized bone particle(DBP)(PLGA/DBP) to investigate the effect of cell viability and proliferation of intervertebral disc(IVD) cells. PLGA is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. DBP contains bioactive molecules. Annulus fibrosus(AF) and nucleus pulposus (NP) cells were seeded in PLGA and PLGA/DBP scaffold to evaluate mRNA expression and a proliferation of AF and NP cells according to content of DBP. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole- 2-l)-2,5-diphenyltetrazolium-bromide(MTT) test. Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression for Type I collagen and Type II collagen of IVD cells in scaffolds. Scaffolds were implanted in subcutaneous of athymic nude mouse for histology in vivo. The formation of disc-like tissue appeared in PLGA/DBP scaffold than control. Especially, 40 and 80 wt% of DBP positively affected to proliferation and maintenance of phenotype in scaffold. These information would contribute to design of PLGA/DBP scaffold for tissue engineered IVD regeneration.