Cytotoxicity and Proteasome Inhibition by Alkaloid Extract from Murraya koenigii Leaves in Breast Cancer Cells—Molecular Docking Studies

Ayesha Ismail,Bindu Noolu,Ramesh Gogulothu,Shyam Perugu,Ajumeera Rajanna,Suresh K. Babu 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.12

Murraya koenigii (curry tree) leaves are rich in bioactive compounds such as flavonoids, alkaloids, and coumarins. Alkaloids from M. koenigii leaves have antianalgesic, antiulcerogenic, antiobesity, and antitumor activities. In this study, we tested the cytotoxic and proteasome-inhibitory potential of a total alkaloid extract (TAE) from M. koenigii leaves in the breast cancer cell line MDA-MB-231. The TAE decreased cell viability with an IC50 of 14.4 μg/mL and altered growth kinetics of breast cancer cells. TAE (32 μg/mL) arrested cells (35%) in the “S” phase of the cell cycle and induced apoptosis. The 26S proteasome, a multicatalytic protease complex, promotes tumor cell proliferation and protects tumor cells from apoptosis. The TAE and mahanine, a carbazole alkaloid present in M. koenigii leaves, preferentially inhibited the trypsin-like, but not the chymotrypsin-like proteolytic activity of the proteasome with an IC50 of 162 μg/mL and 287 μM, respectively. In silico analysis of 26 compounds from M. koenigii leaves revealed significant docking scores for mahanine and two other carbazole alkaloids with the β2 and β5 subunits of the catalytic 20S proteasome. Taken together, this study demonstrates that inhibition of the proteasome is an important biological activity of M. koenigii alkaloids, which may lead to cancer cell death.

Direction- based packet forwarding protocol in Vehicular Content Centric Network

Ayesha Siddiqa,Muhammad Ashar Tariq,Abdueli Paulo Mdee,Huang Yufeng,DongKyun Kim 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.6

M Protein from Dengue virus oligomerizes to pentameric channel protein: in silico analysis study

Ayesha Zeba,Kanagaraj Sekar,Anjali Ganjiwale Korea Genome Organization 2023 Genomics & informatics Vol.21 No.3

The Dengue virus M protein is a 75 amino acid polypeptide with two helical transmembranes (TM). The TM domain oligomerizes to form an ion channel, facilitating viral release from the host cells. The M protein has a critical role in the virus entry and life cycle, making it a potent drug target. The oligomerization of the monomeric protein was studied using ab initio modeling and molecular dynamics simulation in an implicit membrane environment. The representative structures obtained showed pentamer as the most stable oligomeric state, resembling an ion channel. Glutamic acid, threonine, serine, tryptophan, alanine, isoleucine form the pore-lining residues of the pentameric channel, conferring an overall negative charge to the channel with approximate length of 51.9 Å. Residue interaction analysis for M protein shows that Ala94, Leu95, Ser112, Glu124, and Phe155 are the central hub residues representing the physicochemical interactions between domains. The virtual screening with 165 different ion channel inhibitors from the ion channel library shows monovalent ion channel blockers, namely lumacaftor, glipizide, gliquidone, glisoxepide, and azelnidipine to be the inhibitors with high docking scores. Understanding the three-dimensional structure of M protein will help design therapeutics and vaccines for Dengue infection.

Does the Pricing Mechanism Affect the IPO Flipping Activity in Pakistan?

Ayesha ANWAR,Rasidah MOHD-RASHID 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.1

This paper explores the relationship between price mechanism and flipping activity of initial public offerings (IPOs) in Pakistan’s emerging economy. This study uses a cross-sectional data set of 95 firms listed on Pakistan Stock Exchange from 2000 to 2019. This study employs the ordinary least square and quantile regression techniques to capture the relationship between price mechanism and flipping activity. The results show that book-built IPOs flip substantially less than fixed-price IPOs. This is consistent with the signaling theory assertion that roadshows are arranged by underwriters to capture investors’ demand and set the offer prices of IPOs. If investors learn the fair values of quality IPOs, then the offer prices will be close to the intrinsic values, thus reducing flipping. The findings also provide conclusive evidence for understanding the usefulness of and the more relevant information regarding the pricing mechanism. In particular, it provides a better understanding of how companies actually use the pricing mechanism information in the flipping of IPO shares. The results of this study are also valuable to underwriters, and regulators, for instance, provides underwriters with the discretion to allocate the IPO shares and the SECP, in revising regulation on the disclosure of IPO pricing methods.

Policy Perspective Analysis of Technology Adoption in Industries

Ayesha Saleem,Kiyohide Higuchi 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.1

Non-Static Spherically Symmetric Exact Solution of the Einstein-Maxwell Field Equations

Ayesha Mahmood,Azad A. Siddiqui,Tooba Feroze 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.71 No.7

We present a class of exact spherically symmetric and non-static solutions of Einstein-Maxwell’s field equations. We have assumed isotropic pressure distribution and have taken ansatz on two of the gravitational potentials. The solutions admit negative pressure. We show that the solutions satisfy physical boundary conditions associated with the Einstein-Maxwell exact solutions. Therefore, these solutions can model physical systems such as moving dark energy stars.

Transformation of political elite as regime changes in Pakistan

Ayesha Shoukata,Edmund Terence Gomez 서울대학교행정대학원 2018 Asian Journal of Political Science Vol.26 No.1

Burton and Higely [(2001). ‘The study of political elite transformations’, International review of sociology/Revue internationale de sociologie, 11(2): 181–199] argue that when elites of a society are not united this will lead to an unstable political regime. Consensual elite cohesion is only created through distinctive elite transformation. This essay attempts to elaborate their argument in Pakistan, by developing a link among a continuous regime shift from authoritarian to democratic governments and the political elites who keep transforming their structure accordingly. The technique followed for it is analysing the epochal events over time from pre-partition till date which became reason for regime transformations. The discussion focuses on the collusions and contestations of multiple power elites within given social context which are embedded under the international context. It concludes that political elites in Pakistan were always embraced by multiple other power elites who accordingly hold dual elite identity to control the command post in the political arena. Such complex elite structure makes it paradoxical to distinguish political elite of Pakistan from other power elites of Pakistan.

Nepotism Effects on Job Satisfaction and Withdrawal Behavior: An Empirical Analysis of Social, Ethical and Economic Factors from Pakistan

Ayesha SERFRAZ,Zartashia MUNIR,Ahmed Muneeb MEHTA,Md. QAMRUZZAMAN 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.3

Nepotism practices have had a severe impact on the social, ethical, and economic culture of not only corporations but also countries in the modern world. Nepotism behavior not only discourages the determined and motivated employee, but also has a detrimental impact on the worker’s satisfaction, performance, and contribution to the firm’s success. This increases economic tensions due to workers’ dissatisfaction with their jobs and occasionally withdrawal intentions, resulting in poor economic activity and a decline in society as a whole. The purpose of this study is to see how organizations’ nepotism affects individual work happiness, withdrawal behavior, social and ethical values, and the country’s economic development. The study’s target audience was private-sector employees, including managers, non-managerial staff, and middle and lower-level employees. Convenient sampling was used to ensure that the target population was accessible and available. The findings demonstrated that organizational nepotism causes substantial harm not only to employees but also to businesses and the economy. The employee who is happy with his or her job and working environment is less likely to withdraw.

Small Intestinal Bacterial Overgrowth Complicating Gastrointestinal Manifestations of Systemic Sclerosis: A Systematic Review and Meta-analysis

Ayesha Shah,Veenaa Pakeerathan,Michael P Jones,Purna C Kashyap,Kate Virgo,Thomas Fairlie,Mark Morrison,Uday C Ghoshal,Gerald J Holtmann 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.2

Background/AimsSystemic sclerosis (SSc) often is complicated by small intestinal bacterial overgrowth (SIBO). A systematic review and meta-analysis thus examined the prevalence of SIBO in SSc (SSc-subtypes), identify risk factors for SIBO in SSc and the effects of concomitant SIBO on gastrointestinal symptoms in SSc. MethodsWe searched electronic databases until January-2022 for studies providing prevalence rates of SIBO in SSc. The prevalence rates, odds ratio (OR) and 95% confidence intervals (CI) of SIBO in SSc and controls were calculated. ResultsThe final dataset comprised 28 studies with 1112 SSc-patients and 335 controls. SIBO prevalence in SSc-patients was 39.9% (95% CI, 33.1-47.1; P = 0.006), with considerable heterogeneity, (I2 = 76.00%, P < 0.001). As compared to controls, there was a 10-fold increased SIBO prevalence in SSc-patients (OR, 9.6; 95% CI, 5.6-16.5; P < 0.001). The prevalence of SIBO was not different in limited cutaneous SSc as compared to diffuse cutaneous SSc (OR, 1.01; 95% CI, 0.46-2.20; P = 0.978). Diarrhea (OR, 5.9; 95% CI, 2.9-16.0; P = 0.001) and the association between SIBO in SSc and proton pump inhibitor use (OR, 2.3; 95% CI, 0.8-6.4; P = 0.105) failed statistical significance. Rifaximin was significantly more effective as compared to rotating antibiotic in eradicating SIBO in SSc-patients (77.8% [95% CI, 64.4-87.9]) vs 44.8% [95% CI, 31.7-58.4]; P < 0.05). ConclusionsThere is a 10-fold increased prevalence of SIBO in SSc, with similar SIBO prevalence rates in SSc-subtypes. Antimicrobial therapy of SIBO-positive SSc-patients with diarrhea should be considered. However, the results must be interpreted with caution due to substantial unexplained heterogeneity in the prevalence studies, and the low sensitivity and specificity of the diagnostic tests suggesting that the reliability of the evidence may be low.

Whole genome sequence of Staphylococcus aureus strain RMI-014804 isolated from pulmonary patient sputum via next-generation sequencing technology

Ayesha, Wisal,Asad Ullah,Waheed Anwar,Carlos M. Morel,Syed Shah Hassan Korea Genome Organization 2023 Genomics & informatics Vol.21 No.3

Nosocomial infections, commonly referred to as healthcare-associated infections, are illnesses that patients get while hospitalized and are typically either not yet manifest or may develop. One of the most prevalent nosocomial diseases in hospitalized patients is pneumonia, among the leading causes of mortality and morbidity. Viral, bacterial, and fungal pathogens cause pneumonia. More severe introductions commonly included Staphylococcus aureus, which is at the top of bacterial infections, per World Health Organization reports. The staphylococci, S. aureus, strain RMI-014804, mesophile, on-sporulating, and non-motile bacterium, was isolated from the sputum of a pulmonary patient in Pakistan. Many characteristics of S. aureus strain RMI-014804 have been revealed in this paper, with complete genome sequence and annotation. Our findings indicate that the genome is a single circular 2.82 Mbp long genome with 1,962 protein-coding genes, 15 rRNA, 49 tRNA, 62 pseudogenes, and a GC content of 28.76%. As a result of this genome sequencing analysis, researchers will fully understand the genetic and molecular basis of the virulence of the S. aureus bacteria, which could help prevent the spread of nosocomial infections like pneumonia. Genome analysis of this strain was necessary to identify the specific genes and molecular mechanisms that contribute to its pathogenicity, antibiotic resistance, and genetic diversity, allowing for a more in-depth investigation of its pathogenesis to develop new treatments and preventive measures against infections caused by this bacterium.