Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables

C.J. González-Pérez1,I. Vargas-Arispuro,E. Aispuro-Hernández,C.L. Aguilar-Gil,Y.E. Aguirre-Guzmán,A. Castillo,A. Hernández-Mendoza,J.F. Ayala-Zavala,M.A. Martínez-Téllez1 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2

The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.

Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables

( C. J. Gonzalez-perez ),( I. Vargas-arispuro ),( E. Aispuro-hernandez ),( C. L. Aguilar-gil ),( Y. E. Aguirre-guzman ),( A. Castillo ),( A. Hernandez-mendoza ),( J. F. Ayala-zavala ),( M. A. Martinez 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.2

The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.

Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables

Gonzalez-Perez, C.J.,Vargas-Arispuro, I.,Aispuro-Hernandez, E.,Aguilar-Gil, C.L.,Aguirre-Guzman, Y.E.,Castillo, A.,Hernandez-Mendoza, A.,Ayala-Zavala, J.F.,Martinez-Tellez, M.A. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2

The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.

Nutraceutical effects of bioactive peptides obtained from Pterophylla beltrani (Bolivar & Bolivar) protein isolates

Laura Jenet Montiel-Aguilar,Jorge Ariel Torres-Castillo,Rocío Rodríguez-Servin,Adiel Berenice López-Flores,Víctor Eustorgio Aguirre-Arzola,Gerardo Méndez-Zamora,Sugey Ramona Sinagawa-García 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3

Edible insects have been important sources of food proteins for human consumption and animal feed. In this study, a protein isolate from Pterophylla beltrani Bolivar & Bolivar, 1942 (Orthoptera: Tettigoniidae) was enzymatically processed and its nutraceutical properties were evaluated. Protein isolates were obtained from an insect flour and then was hydrolyzed for 5 h with a sequential process using pepsin and trypsin-chymotrypsin to simulate the gastric intestinal fluids. To evaluate the effect of peptide molecular size on nutraceutical properties, the peptides obtained from Total hydrolyzed (TH) were fractionated by ultrafiltration (UF) with 10 kDa and 3 kDa UF membranes giving fractions (F) with different molecular size (F < 3KDa, F < 10KDa and F > 10KDa). The inhibition assay of Angiotensin-Converting Enzyme (ACE) showed that the best treatment (P < 0.05) was the (TH) with an IC50 value of 0.5 mg/mL while the F < 3KDa was the lowest (P < 0.05) with an IC50 of 1.44 mg/mL, and the peptide size had no effect. However, an α-amylase inhibition was observed with an increase of the IC50 value between TH, F > 10KDa and F < 10KDa although no significative difference (P > 0.05) was found between the TH and F < 3KDa with IC50 of 0.48 and 0.68 mg/mL, respectively. In antioxidant activity, significant differences (P < 0.05) were observed between TH and UF fractions where the best response was in the F < 3KDa. In conclusion, P. beltrani proteins isolate are a source of bioactive peptide, and these could be considered as potential edible insect and sustainable food with nutraceutical effects.

Development of Perovskite-type Cobaltates and Manganates for Thermoelectric Oxide Modules

Weidenkaff, A.,Aguirre, M.H.,Bocher, L.,Trottmann, M.,Tomes, P.,Robert, R. The Korean Ceramic Society 2010 한국세라믹학회지 Vol.47 No.1

Ceramics with perovskite-type structure are interesting functional materials for several energy conversion processes due to their flexible structure and a variety of properties. Prominent examples are electrode materials in fuel cells and batteries, thermoelectric converters, piezoelectrics, and photocatalysts. The very attractive physical-chemical properties of perovskite-type phases can be modified in a controlled way by changing the composition and crystallographic structure in tailor-made soft chemistry synthesis processes. Improved thermoelectric materials such as cobaltates with p-type conductivity and n-type manganates are developed by following theoretical predictions and tested to be applied in oxidic thermoelectric converters.

Half-cycle atomic layer deposition reaction studies of Al₂O₃ on In_0.2Ga_0.8As (100) surfaces (3 pages)

Milojevic, M.,Aguirre-Tostado, F.S.,Hinkle, C.L.,Kim, H.C.,Vogel, E.M.,Kim, J.,Wallace, R.M. AMERICAN INSTITUTE OF PHYSICS 2008 APPLIED PHYSICS LETTERS Vol.93 No.20

Development of Perovskite-type Cobaltates and Manganates for Thermoelectric Oxide Modules

A. Weidenkaff,M.H. Aguirre,L. Bocher,M. Trottmann,P. Tomes,R. Robert 한국세라믹학회 2010 한국세라믹학회지 Vol.47 No.1

Ceramics with perovskite-type structure are interesting functional materials for several energy conversion processes due to their flexible structure and a variety of properties. Prominent examples are electrode materials in fuel cells and batteries, thermoelectric converters, piezoelectrics, and photocatalysts. The very attractive physical-chemical properties of perovskite-type phases can be modified in a controlled way by changing the composition and crystallographic structure in tailor-made soft chemistry synthesis processes. Improved thermoelectric materials such as cobaltates with p-type conductivity and n-type manganates are developed by following theoretical predictions and tested to be applied in oxidic thermoelectric converters.

Half-cycle atomic layer deposition reaction studies of Al₂O₃ on (NH₄)₂S passivated GaAs(100) surfaces (3 pages)

Milojevic, M.,Hinkle, C.L.,Aguirre-Tostado, F.S.,Kim, H.C.,Vogel, E.M.,Kim, J.,Wallace, R.M. AMERICAN INSTITUTE OF PHYSICS 2008 APPLIED PHYSICS LETTERS Vol.93 No.25

Pig meat production in the European Union-27: current status, challenges, and future trends

Mateos G. G.,Corrales N. L.,Talegón G.,Aguirre L. 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

The main objective of this study was to present data on the current situation and future trends of pig meat production in the European Union-27 (EU). Pig production has played an important social and economic role for centuries in many states of the EU. In 2022, pig meat production in the EU reached 23 M tons, which represented 21% of total production worldwide. The two key reasons that justify such amount of pork produced, are the acceptance and high consumption of the meat by the local population and the high quality of the meat produced which facilitated pork export. However, current data show a reduction in pork production for the last three years, as a consequence of a series of events that include i) problems with the chain of ingredients supply, ii) uncontrolled increase in African Swine Fever (ASF) outbreaks, iii) fast recovery of pig production in China, iv) increasing concerns by the rural population on the high cost to meet future requirements of the EU legislation on farm management, environmental sustainability and animal welfare, v) increased cost of all inputs involved in pig production and vi) limited interest of the new farmer generation to work on the pig sector. Consequently, pork production is expected to decrease in the EU for the next years, although sales will be maintained at a relative high level because pork is the meat preferred by local consumers in most EU countries. In order to maintain the favourable position of the pork industry in the near future, strategies to implement include: i) maintain the quality of the meat destinated to export markets, ii) improve the control of outbreaks of ASF and other swine diseases, iii) implementation of technological innovations to improve working conditions making more attractive to work in the pork sector of the food chain to the new generation of farmers and workers.

Remote sensing based forest canopy opening and their spatial representation

Tania Fernández Vargas,Trejo Vázquez Irma,Aguirre Gómez Raúl 한국산림과학회 2021 Forest Science And Technology Vol.17 No.4

The use of remote sensing in natural resource management is an easily accessible input for obtaining detailed information on the ground and landscape. There is a wide range of procedures to analyze the forest canopy through satellite images. The purpose of this work is to obtain a map of forest opening with remote sensing by relating several vegetation indices, Kauth-Thomas transformation and texture filters, to a Landsat 8OLI image. A factor analysis was made to evaluate the contribution of these variable to identify the opening of the forest cover, yielding a r2 ¼ 76%. The results show that the Modified Soil Adjusted Vegetation Index (MSAVI), Soil Adjusted Vegetation Index (SAVI), and brightness factor have the best correlation (0.225–0.216 component coefficient). The resulting model was reclassified into five categories of forest opening and associated with land use data from the National Institute of Statistics and Geography (INEGI-M exico). Thus, 95% of human settlements have a canopy opening between medium and very high, the crops areas 72%, and the low deciduous forest with secondary shrub vegetation 100% of the opening. Coniferous and mixed forests have a low to very low canopy opening 46% and 55%, respectively of their surface. The forests with secondary vegetation, both shrub and arboreal, present greater openness than the same forests in the primary state. Verification of the spatial representation data of canopy opening was made by comparing 94 hemispheric photographs with 94 sites located in open areas obtaining an r ¼ 0.57. This work offers a simple and straightforward methodology, easily replicable in different types of vegetation using free satellite imagery. Hence, it is a helpful tool for decision-makers when considering the general status of conservation of forest systems and their spatial distribution.