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Abdollah Khadivi-Khub,Zabihollah Zamani,Naser Bouzari 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.3
Sweet cherry (Prunus avium L.), a member of Rosaceae family, is an economically important fruit of the temperate zone. In Iran, various sweet cherry genotypes are grown in different areas. For estimation of genetic diversity, 23 RAPD decamer primers data as well as 23 morphological traits were used on 39 sweet cherry cultivars and genotypes, 28 of Iranian and 11 of foreign origin. Random Amplified Polymorphic DNA (RAPD) data was used for clustering of genotypes using UPGMA method. Based on the results, in some cases, clustering of genotypes by RAPD data was in agreement with morphological data; however, the correlation between the two sets of data was not significant (r = 0.2). The coephenitic coefficients between genotypes varied from 0.43 to 0.83 and the value of calculated polymorphism was 81.7 percent, indicating the presence of a high variation between the studied cultivars. This could be due to the presence of both Iranian and foreign genotypes in the experiment. In the main subcluster, genotypes from both origins were present and some genotypes were showing close relationships. Significant regression associations were found between 7 morphological traits and RAPD markers and some informative markers were found for the traits. Also, in clustering of genotypes good agreements were found between the subclusters and the pollination incompatibility groups reported by other workers. The results showed that RAPD is an effective maker for study of genetic diversity among sweet cherry genotypes.
Effect of Different Budding Methods and Times on Grafting Success of Walnut
Nosrati, Zia,Khadivi-Khub, Abdollah Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.6
Vegetative propagation of walnut is difficult compared with that of other fruit and nut species. The present study assessed three methods of grafting (patch, shield, and chip) at various periods of walnut growth and with different timings of grafting in walnut. Early May was the best time for grafting, at which time the highest success rate was obtained by the patch method (96%), followed by chip-budding (75%), while shield-grafting showed the lowest efficiency (10%). Patch-grafting was also successful (75-80%) in early August and moderately successful in mid-June (51-55%), while the shield and chip methods had no success during these two times (0.00%). Patch-grafting was more efficient and also induced better callus formation and scion growth than the other two methods. The genotypes used did not affect grafting efficiency. The best results were obtained by patch-budding in both tested genotypes. The present findings show the potential value of patch-grafting in early May as a propagation method for walnut for establishment of guidelines for propagation.
Effect of Different Budding Methods and Times on Grafting Success of Walnut
Zia Nosrati,Abdollah Khadivi-Khub 한국원예학회 2014 원예과학기술지 Vol.32 No.6
Vegetative propagation of walnut is difficult compared with that of other fruit and nut species. The present study assessed three methods of grafting (patch, shield, and chip) at various periods of walnut growth and with different timings of grafting in walnut. Early May was the best time for grafting, at which time the highest success rate was obtained by the patch method (96%), followed by chip-budding (75%), while shield-grafting showed the lowest efficiency (10%). Patch-grafting was also successful (75-80%) in early August and moderately successful in mid-June (51-55%), while the shield and chip methods had no success during these two times (0.00%). Patch-grafting was more efficient and also induced better callus formation and scion growth than the other two methods. The genotypes used did not affect grafting efficiency. The best results were obtained by patch-budding in both tested genotypes. The present findings show the potential value of patch-grafting in early May as a propagation method for walnut for establishment of guidelines for propagation.
Influence of Apical Meristem Culture and Thermotherapy on Production of Healthy Fig Plants
Amir Sahraroo,Mesbah Babalar,Ali Ebadi,Mina Kouhi Habibi,Abdollah Khadivi-Khub 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
In this study, production of healthy fig plant using apical meristem culture and thermotherapy in fig (Ficus carica L. cv. Jaami-e-Kan) was conducted. At first, apical meristem (0.2-0.4 and 0.5-0.7 ㎜ in size) of the plant with evident fig mosaic disease (FMDs) symptoms were developed on MS solid medium supplemented with 0.5 ㎎ㆍℓ<SUP>-1</SUP> 6-benzyladenine (BA) and 0.1 ㎎ㆍℓ<SUP>-1</SUP> α-naphthalene acetic acid (NAA). In addition, nodal segment from meristemic microshoots were proliferated on MS medium supplemented with BA (1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>) and NAA (0, 0.1 and 0.5 ㎎ㆍℓ<SUP>-1</SUP>). Finally, the plantlets were rooted on half strength MS medium containing IBA (0.5, 1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>). Significant differences were observed between the treatments. Smaller meristems (0.2-0.4 ㎜) showed the best result in removing the FMDs symptoms after 12 months (93%). Shoot proliferation rate was found to be higher in the medium containing 2 ㎎ㆍℓ<SUP>-1</SUP> BA than other concentrations. Highest numbers of rooted plantlets were obtained from 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP> IBA. At the second experiment, potted plantlets with evident symptoms were subjected with two alternating high temperature regime (35/40℃ and 30/35℃ dark/light) and were evaluated after 4 and 6 weeks. Thermotherapy regime with 35/40℃ dark/light during 6 weeks was significantly (p<0.05) effective on removing the FMDs symptoms (98%).