http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
해수 사육 무지개송어(Oncorhynchus mykiss)의 Viral Hemorrhagic Septicemia Virus (VHSV) 모니터링
김위식,공경희,전영호,오명주,Kim, Wi-Sik,Kong, Kyoung-hui,Jeon, Young-Ho,Oh, Myung-Joo 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
Mariculture of rainbow trout Onchorhynchus mykiss has been initiated in or around olive flounder Paralichthys olivaceus farms, where viral hemorrhagic septicemia virus (VHSV) is often detected in some fish. In the present study, we investigated VHSV infection in seawater-reared rainbow trout because VHSV has never been detected in salmonids in Korea. A total of 104 adult fish were tested for the presence of VHSV by reverse transcription-polymerase chain reaction, followed by virus isolation with the fathead minnow caudal trunk cell line. Cytopathic effects were observed in two samples but the virus was identified as infectious hematopoietic necrosis virus. Thus, VHSV was not isolated from seawater-reared rainbow trout.
김위식 ( Wi Sik Kim ),김정 ( Jung Kim ),황두진 ( Doo Jin Hwang ),한종석 ( Jong Seok Han ),이시우 ( Si Woo Lee ),최동익 ( Dong Ik Choi ),임상민 ( Sang Min Lim ),오명주 ( Myung Joo Oh ) 한국어병학회 2012 한국어병학회지 Vol.25 No.2
Exfoliation of fouling abalone, Haliotis discus hannai from shelters by commercial oxytetracycline (OTC) was observed in culture farms. In the present study, different components of OTC for exfoliation of abalone were investigated to understand how to work. Abalone were detached from shelter in 14,000 ppm of commercial OTC (main ingredients of OTC: OTC-hydrogen chloride (HCl), 50%; glucose, 49%; blue pigment, <1%), but not below 8,000 ppm. A 95% of exfoliation rate was observed in OTC-HCl (7,000 ppm, pH 2.8) but no exfoliation in OTC-HCl (7,000 ppm, pH 5.0), glucose (7,000 ppm) or blue pigment (140 ppm). Moreover 100% exfoliation rate was observed in HCl-seawater of pH 2.8. These results indicate that HCl is the component resulting in exfoliation of the fouling abalone. Abalone was detached in HCl solution (pH 2.5-3.2) within 2 min. However, a lower pH and longer treatment resulted in delayed recovery of the detached abalone. Thus, exfoliation of fouling abalone can be achieved by a low pH treatment with cautious handling.
김위식 ( Wi Sik Kim ),장민석 ( Min Seok Jang ),정성주 ( Sung Ju Jung ),김석렬 ( Seok Ryel Kim ),박명애 ( Myoung Ae Park ),이정호 ( Jeong Ho Lee ),명정인 ( Jeong In Myeong ),오명주 ( Myung Joo Oh ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1
The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of 15℃, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24∼48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94∼3.04. At the end of the experiment (84 days), 0.03∼1.28 OD values were observed. In the rearing temperature of 12∼13℃, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88∼2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of 10℃, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37∼1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.
2000~2010년 우리나라 양식산 뱀장어, Anguilla japonica의 병원체 감염현황
김위식 ( Wi Sik Kim ),옥하나 ( Ha Na Ok ),김도형 ( Do Hyung Kim ),김흥윤 ( Heung Yun Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2011 한국어병학회지 Vol.24 No.3
Disease survey was conducted to investigate the cause of high mortality in 23 farms of eel, Anguilla japonica, during the period from 2000 to 2010. Seven kinds of fish pathogens were confirmed in the tested fish, which included: Pseudodactylogyrus sp. (infection rate: 65.5%, 19/29 samples), aquabirnavirus (ABV, 62.5%, 15/24 samples), Edwardsiella sp. (44%, 11/25 samples), anguillid herpesvirus 1 (AngHV1, 16.7%, 2/12 samples), Heterosporis sp. (10.3%, 3/29 samples), Vibrio sp. (8%, 2/25 samples), Trichodina sp. (3.4%, 1/29 samples). The rate of single infection was 44.8% (13/29 samples), while 62.1% (18/29 samples) showed mixed type of infection with 2 to 5 different pathogens. The most predominant mixed infection were Pseudodactylogyrus sp.-Edwardsiella sp. (10.3%, 3/29 samples), Pseudodactylogyrus sp.-ABV (10.3%, 3/29 samples), Edwardsiella sp.-ABV (6.9%, 2/29 samples). Infection by the above infectious parasites, bacteria and virus was confirmed in tested eels of juvenile to adult sizes that were cultured at 25-30℃ in almost the examined farms.
2010∼2012년 연안에서 서식하는 해산어에서 아니사키스 유충의 감염현황
김위식 ( Wi Sik Kim ),전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),김도형 ( Do Hyung Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
A survey was conducted to investigate infection of anisakid nematode larvae in 243 wild marine fishes caught from the southern coastal area of Korea between 2010 and 2012. The samples comprised fishes from 9 orders, 30 families and 50 species. Total infection rate of anisakid nematode larvae was 10.7% (26/243 fish), which comprised from Yeosu, 7.4% (7/95) in 2010 and 22.7% (5/22) in 2011; from Jeju, 8.2% (5/61) in 2011; from Wando, 40.9% (9/22) in 2012. Anisakid nematode larvae were not detected in Tongyoung and Wando samples in 2011. Molecular identification of the 89 worms from 26 fish was conducted by PCR-RFLP and/or sequence analysis of internal transcribed spacer (ITS) region of ribosomal DNA. From the results, 6 kinds of anisakis species were identified: Anisakis pegreffii (infection rate: 53.9%, 48/89 worms), Hysterothylacium aduncum (38.2%, 34/89), H. fabri (3.4%, 3/89), hybird (A. simplex X A. pegreffii) (2.4%, 2/89), A. simplex (1.1%, 1/89) and Raphidascaris lophii (1.1%, 1/89). The rate of single infection was 80.8% (21/26 infected fish), while 19.2% (5/26) showed mixed infection with 2 to 3 different anisakis species.
김위식 ( Wi Sik Kim ),전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was evaluated to monitor infectious hematopoietic necrosis virus (IHNV) from artificially infected rainbow trout Oncorhynchus mykiss. The cumulative mortalities of fish challenged with IHNV at 106.5 TCID50/fish, 105.5 TCID50/fish and 104.5 TCID50/fish were 40%, 0% and 0%, respectively. Dead fish and survivors at 16 and 28 d post-challenge in each group were employed for IHNV detection by RT-LAMP assay and virus isolation using BF-2 cells. IHNV from 104.3 to 106.8 TCID50/㎖ was isolated from all the dead fish and also detected in all of the examined dead fish by RT-LAMP assay. In survivors at 16 d, 60% (3/5 fish, 102.8-105.05 TCID50/㎖), 20% (1/5 fish, 101.05 TCID50/㎖) and 60% (3/5 fish, 101.05-104.8 TCID50/ ㎖) were found to be IHNV-positive by virus isolation in fish challenged with IHNV at 106.5 TCID50/fish, 105.5 TCID50/fish and 104.5 TCID50/fish, respectively, while 20% (1/5 fish), 0% (0/5 fish) and 20% (1/5 fish) were IHNV-positive by RT-LAMP assay. No IHNV was detected in the survivors at 28 d and control fish. These results indicate that the RT-LAMP assay is useful for detection of IHNV in diseased fish although it is not enough to monitor virus in IHNV-survivors.
김위식 ( Wi Sik Kim ),이시우 ( Si Woo Lee ),김정 ( Jung Kim ),최동익 ( Dong Ik Choi ),오명주 ( Myung Joo Oh ),황두진 ( Doo Jin Hwang ) 한국어병학회 2013 한국어병학회지 Vol.26 No.1
It is reported that abalone, Haliotis discus hannai, was detached from shelters by commercial oxytetracycline (OTC) dissolved in hydrochloric acid (HCl). In the present study, we investigated the exfoliation effect of fouling abalone by organic acids instead of OTC or HCl. Organic acids (malic acid, citric acid, lactic acid and formic acid) of pH 2.6 and pH 2.1-2.3 exfoliated over 67.6% and 91.7% of abalone, respectively; while OTC of pH 2.6 and pH 2.1-2.3 exfoliated 25.9% and over 74.1% of abalone, respectively. These results indicate that the exfoliation effect of organic acid is better than that of OTC dissolved in HCl at the same pH. However, a lower pH and longer treatment of organic acids resulted in delayed recovery of the detached abalone; abalone immersed in pH 2.3 for 10 second was recovered within 5 min, but took 12 min to recover after 30 second immersion. Moreover, recovery period for abalone exposed to pH 2.1 for 30 second was at least 15 min 45 second. In conclusion, though acids need to be cautiously handled, organic acids may be a better candidate to detach abalone instead of OTC or HCl.
김위식 ( Wi Sik Kim ),강민호 ( Min Ho Kang ),김종오 ( Jong Oh Kim ),이시우 ( Si Woo Lee ),김정 ( Jung Kim ),황두진 ( Doo Jin Hwang ),오명주 ( Myung Joo Oh ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2
Three commercial exfoliating reagents, product A (main components: citric acid and vitamin C), B (herb) and C (nicotinamide), were used to study their exfoliation effect on abalone, Haliotis discus hannai from the substrate. The exfoliating reagents A, B, C and oxytetracycline (OTC, control) of 6-31.25 g/L and 12-37.5 g/L exfoliated 81.2-84.8% and 90.3-95% of abalone, respectively. Post-treatment recovery time for the abalone was similar for all the reagents except product C. Recovery period for the abalone immersed in OTC for 5-20 second was slightly shorter than the reagents A, B and C; however, no mortality was observed in any group except with the reagents B (concentration: 20 g/L, immersion time: 5 sec) and C (12 g/L, 10 and 20 sec) that showed negligible mortality of 3.3%. Higher concentration and longer treatment with the reagents resulted in longer recovery time of the detached abalone. Although abalone exposed to the reagents needs slightly longer time to recover than that to OTC, the exfoliation effect is much similar. These results indicate that the commercial exfoliating reagents can replace OTC to detach abalone, though they need to be cautiously handled.
김위식 ( Wi-sik Kim ),김종오 ( Jong-oh Kim ),오명주 ( Myung-joo Oh ) 한국어병학회 2017 한국어병학회지 Vol.30 No.1
Twenty nine edible plants were selected in order to study their exfoliation effect on abalone Haliotis discus hannai from its substrate. Exfoliation was observed in spearmint Mentha spicata, lemon balm Melissa officinalis, rose flower, onion Allium cepa, wasabi powder, mustard powder, omija Schizandra Chinensis, and hibiscus Hibiscus sabdariffa solutions. In particular, both the omija and hibiscus solutions exfoliated over 90% of the abalone. In field tests, 3% and 4% omija solutions exfoliated 93.6% (850/908) and 97.1% (810/834) of abalone, respectively, whereas 3% and 4% hibiscus solutions exfoliated 96.1% (780/812) and 97.4% (700/719), respectively. The detached abalone were recovered within 1 min 39 seconds. These results indicate that the omija and hibiscus solutions (3% and 4%) may be useful in the exfoliation of abalone.
조피볼락(Sebastes schlegeli )에 대한 비정형 Aeromonas salmonicida 포르말린 사균 백신의 효과
김위식 ( Wi-sik Kim ),이현호 ( Hyeon-ho Lee ),오명주 ( Myung-joo Oh ),한현자 ( Hyun-ja Han ) 한국어병학회 2018 한국어병학회지 Vol.31 No.1
Atypical furnculosis caused by atypical Aeromonas salmonicida, is an emerging problem of farming of rockfish (Sebastes schlegeli) in Korea. In this study, protection against atypical furunculosis was compared in rockfish vaccinated with A. salmonicida formalin killed cell (FKC) and adjuvant containing FKC. The formalin inactivated A. salmonicida vaccine provided a low protection of 20% and 10% relative percent survival (RPS) at 44 and 58 days post vaccination. However, addition of adjuvant (squalene and aluminum hydroxide) into inactivated A. salmonicida vaccine clearly enhanced the level of protection showing 70% and 50% RPS at 44 and 58 days post vaccination.