Neomysis awatschensis의 성장과 에너지 수지

김흥윤,진평,Kim, Heung-Yun,Chin, Pyung 한국통합생물학회 1991 동물학회지 Vol.34 No.4

연안 천해에 서식하는 반부유성 갑각류인 감eomysis awatschensis를 수온 16$^{\circ}C$와 $25^{\circ}C$에서 Anemiusalina유생을 먹이로 공급하며 사육하면서 성장과 탈피에 미치는 수온의 영향을 조사, 성장, 탈피, 생식 및 호흡에 쓰인 에너지량과 체를필의 화학적 조성 및 에너지 함량을 측정한 자료로부터 개체의 사용 에너지 수지를 계산하였다. N. awatschensis 자웅의 총평균 체장성장율은 16$^{\circ}C$에서 0.08 mm/day, $25^{\circ}C$에서는 0.12maydaY였다. 수온은 동물의 각 탈피간 체장증가 백분율(growth factor)에는 영향을 미치지 않았으나 탈피기간에는 뚜렷한 영향을 나타내었다. 암컷의 체장별 각 탈피간 체장증가율은 수컷에 비하여 평균 3oye 정도 높았다. 성장과 탈피에 사용하는 평균 에너지는 각각 5.55 cal/mg X (건중, mg) 및 0.337 col/mg X (건중, mg)이였다. 난 1개의 에너지 함량은 0,057 cal이고, 암컷이 생식에 사용하는 에너지는 체장별 포란수와 관련되었다. 호흡계수는 4.5 col/ml 02였고, 회분비방법에 의한 동화효율은 70.9%였다. N. owotschensis의 총사용 에너지 중 성장에 사용하는 에너지는 갑각류의 다른 종에 비하여 높았다.

수온과 저산소에 따른 조피볼락(Sebastes schlegeli)의 호흡대사와 혈액성상의 변화

김흥윤 ( Heung-yun Kim ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2

본 연구는 수온 15, 20 및 25℃에서 조피볼락(Sebastes schlegeli)을 점진적인 저산소 조건에 노출시켜 호흡 대사율이 표준대사율(SMR) 이하로 감소하는 산소포화도, S_crit를 평가하고, 수온별 S_crit에 4시간 노출 후 혈액 성상의 변화를 조사하기 위하여 실시하였다. 수온 15, 20, 25℃에서 normoxic SMR은 각각 116.5±5.5, 188.6±4.2 및 237.4±6.8 mg O₂/kg/hr이었고, 호흡계수(Q₁₀)는 15-20℃ 구간에서는 2.62, 20-25℃에서는 1.58, 그리고 전 수온 범위의 15-25℃에서는 2.04였다. 수온 15, 20 및 25℃에서 Scrit는 각각 22.1±1.2, 30.6±1.5 및 41.9±1.4%였다. 수온별 S_crit에 4시간 노출 후 혈액 Ht와 Hb 및 혈장 cortisol, glucose, 전해질(Na⁺, K⁺, Cl^-)과 삼투질 농도는 모든 수온에서 normoxic water의 대조구에 비하여 유의하게 높았다. 수온 15, 20, 25℃의 normoxic water에서는 Ht, Hb 및 혈장 glucose 농도는 15℃에 비하여 20℃와 25℃에서, 혈장 cortisol, 전해질 및 삼투질 농도는 25℃에서 유의하게 높았다. 수온별 S_crit의 저산소에 노출된 조피볼락은 수온이 높을수록 저산소에 의한 어체의 생리적 스트레스는 가중되는 것으로 나타났다. Experiments were performed to investigate changes in metabolic rate (MO₂), critical oxygen saturation (Scrit) and hematological parameters of black rockfish, Sebastes schlegeli exposed to hypoxia at 15, 20 and 25℃. The MO₂ was measured at an interval of 10 min using intermittent-flow respirometry. The normoxic standard metabolic rate (SMR) was 116.5±5.5, 188.6±4.2 and 237.4±6.8 mg O₂/kg/hr, and Scrit was 22.1±1.2, 30.6±1.5 and 41.9±1.4% air saturation at 15, 20 and 25℃, respectively. Q₁₀ values were 2.62 between 15 and 20℃, 1.58 between 20 and 25℃, and 2.04 over the full temperature range. In the investigation of blood (hematocrit and hemoglobin) and biochemical parameters (plasma cortisol, glucose, electrolyte and osmolality), the rockfish were subjected to Scrit for each temperature during 4 hr. All of hematological parameters of the rockfish exposed to hypoxic water were significantly higher than those of normoxic control. Moreover, blood and biochemical parameters of the rockfish maintained to normoxic water showed the tendency of increase with temperature, and were significantly higher at 25C. As a result of this experiment, it was found that physiological stress due to hypoxia increased at high temperature.

표면전처리가 반응성 스퍼터링법으로 제조한 TiN 코팅층의 밀착력에 미치는 영향

김흥윤(Heung-Yun Kim),백운승(Woon-Seung Beak),권식철(Sik-Chol Kwon),김규호(Kyu-Ho Kim) 한국표면공학회 1993 한국표면공학회지 Vol.26 No.5

유자 첨가 사료로 사육된 넙치의 영양성분

김흥윤 ( Heung Yun Kim ),김은희 ( Eun Heui Kim ),김도형 ( Do Hyung Kim ),오명주 ( Myung Joo Oh ),신태선 ( Tai Sun Shin ) 한국수산학회 2009 한국수산과학회지 Vol.42 No.3

This study was conducted to investigate the effect of diets supplemented with different levels (0, 2.5, 5.0, and 7.5%) of yuza (Citrus junos Sieb ex Tanaka) on nutritional composition of olive flounder. Four groups of fish (242.2±14.2g) were fed to apparent satiation twice daily for 4 months. There were no significant differences in proximate composition among the treatment groups (P<0.05). Vitamin C content in flounder muscle was higher in the yuza-added groups than in the control group, and the content among the treatment groups increased as amount of yuza added to diets increased (P<0.05). Of the eight organic acids in flounder muscle, lactic acid was predominant, followed by oxalic acid, succinic-acid, tartaric acid, and acetic acid. Flounders fed 2.5% yuza diet had the highest lactic acid content of all treatments. Four sugars were found in all groups and glucose was the major sugar. Glucose and ribose were detected as the highest sugars in the 2.5% treatment, while maltose and galactose were the dominant sugars in the 5.0% treatment. The abundant fatty acids in fed flounders were 22:6n-3 (DHA), 16:0, and 18:1n-9, which were composed of over 60% of total fatty acids. The control and the 7.5% treatment group had higher 22:6n-3 (DHA) content than the other groups. Major amino acids in samples were glutamic acid, aspartic acid, lysine, leucine, valine, arginine, and alanine. The 2.5% yuza treatment had the highest content of total amino acids and essential amino acids. There were little differences in the free amino acid compositions among the treatments. However, taurine was the predominant amino acid and made up over 47% of total free amino acids. The 2.5% added yuza group contained higher amount of sweet amino acids such as alanine, serine, proline, glycine than the other groups. The addition of yuza to diet of olive flounder had no or little effect on the nutritional components of olive flounder except for vitamin C. However, the 2.5% yuza added group had the highest nutritional values of the treatment groups.

이산화염소(ClO₂) 처리해수에 노출된 넙치, Paralichthys olivaceus의 생존과 생리적 반응

김흥윤(Heung-Yun Kim),김진도(Jin-Do Kim) 한국수산과학회 양식분과 2003 韓國養殖學會誌 Vol.16 No.3

광양만 주변 해역의 수산자원 분포 및 계절적 변화

김흥윤(Heung-Yun KIM),한인우(Inwoo HAN),오우석(Wooseok OH),최정화(Junghwa CHOI),이경훈(Kyounghoon LEE) 한국수산해양교육학회 2018 水産海洋敎育硏究 Vol.30 No.6

수온급변에 노출된 참돔의 생존과 생리적 반응

김흥윤(Heung Yun KIM) 전남대학교 수산과학연구소 2004 수산과학연구소논문집 Vol.13 No.-

This experiment was conducted to estimate the effects of sudden changes in water temperature (WT) on survival and physiological responses (hematocrit: Ht, hemoglobin: Hb, serum cortisol and glucose levels, and osmolality) of red seabream, Pagrus major. All the fishes were acclimated for 2 weeks at 25℃ before experiment initiation, thereafter exposed to each WT regime (13, 16 and 19℃) in a flow through recirculation culture system with 12 tanks (water vol. 200 L/tank). The WT was decreased from 25℃ to 13, 16 and 19℃ within 6 hours (2, 1 and 0.5℃/hour), and then was increased to 25℃ within 6 hours after maintenance for 42 hours in each WT regime. The cumulative survival rates of the fish exposed to the experimental WT regime of 13℃ and 16℃ during whole experiment were shown 0% and 88%, respectively. The blood Hct and Hb of 16℃-exposure fish were significantly decreased and were significantly increased in the level of serum cortisol and glucose as compared with those in the control. The Hct, Hb, cortisol and glucose levels of the fish exposed to 16℃ were recovered after back to initial temperature of 25℃. The serum cortisol and glucose levels of all fish groups were significantly increased by lowering WT, and were shown the hypercortisolism and hyperglycemia in the 13℃-exposure fish group.

참전복 , Haliotis discus hannai 치패의 생존과 산소소비에 미치는 phenol 의 독성 영향

김흥윤(Heung Yun Kim) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3

This study was carried out to estimate toxic effects of phenol on survival and metabolism of the abalone juvenile, Haliotis discus hannai. The experiment was conducted by renewal bioassay procedure with different salinities at 20℃. The LC_(50) of the juvenile exposed to phenol in the range of 0.5 and 100㎎/ℓ was 34.3-6.5㎎/ℓ at 24‰ and 52.2-9.3㎎/ℓ at 32‰ salinity with exposure time from 24 hours to 96 hours. LT_(50) was remarkablely reduced with increase of phenol concentration and decrease of salinity. Lethal toxicity of phenol was higher at low salinity than at high salinity. Therefore, salinity is likely to be one of factor to increase phenol toxicity. The oxygen consumption of the juvenile was reduced with increase of phenol concentration and with decrease of salinity. In spite of phenol toxicity, the oxygen consumption of the juvenile exposed to phenol of low concentration was high and similar as compared with that of control group. Survival rates of the abalone kept in phenol-free sea water after exposure to phenol concentration of 5, 10 and 20㎎/ℓ for 96 hours were reduced with decrease of salinity. Durations required to recover the normal metabolic rate of the juvenile, which was exposed to phenol concentration of 5, 10 and 20㎎/ℓ for 96 hours, were made longer with increasing phenol concentration. In the case of the juvenile exposed to subtethal concentration of phenol for 15 days, it were elongated as compared with that of the abalone exposed to phenol concentration caused acute toxicity. The result of this experiment indicated that relatively low concentration of phenol can impact on the abalone juvenile in marine ecosystem.

유자 첨가 사료로 사육된 넙치의 휘발성 향미 성분

김흥윤 ( Heung Yun Kim ),신태선 ( Tai Sun Shin ) 한국수산학회 2009 한국수산과학회지 Vol.42 No.3

Volatile components in Olive Flounder fed diets containing 0, 2,5, 5.0, and 7.5% yuza (Citrus junos Sieb ex Tanaka) for 4 months were investigated. Samples were extracted by solid-phase microextraction and analyzed by gas chromatography/mass spectrometry. Among 89 compounds detected, 82 were positively identified. Volatile compounds of Olive Flounder fed the unsupplemented diet comprised 12 acids, 10 alcohols, eight aldehydes, five aromatic compounds, nine esters, 12 hydrocarbons, four ketones, two monoterpenes, and one miscellaneous compound. Compounds identified in Olive Flounder fed the yuza-supplemented diets consisted of 10 esters, 11 monoterpenes, 13 sesquiterpenes, and two miscellaneous compounds, with the other compounds being the same as in the control. The most abundant class of compounds in flounders fed the yuza-supplemented diet was the monoterpenes, which included limonene, β-terpinene, β-trans -ocimene, and α-terpinolene. Of the 13 sesquiterpenes identified in flounder fed the yuza-supplemented diet, bicyclogermacrene was the major volatile compound followed by allo-aromadendrene, trans-caryophyllene, and δ-cadinene. Bicyclogermacrene and germacrene D content increased significantly as the yuza supplementation increased.

감성돔 ( Acanthopagrus schlegeli ) 의 초기생활사 동안 소화기관 발달

이정식(Jung Sick Lee),김흥윤(Heung Yun Kim),변순규(Soon Gyu Byun),김진도(Jin Do Kim),고창순(Chang Soon Go),진평(Pyung Chin) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.2

Differentiation and development of the digestive organ of the black sea bream, Acanthopagrus schlegeli were studied by means of histological methods. The hatched larva in TL (total length) 2.0㎜ (n=10) had a yolk sac of 1,000×590㎛ and simple straight digestive tract, which was composed of cuboidal epithelium. In the pre-larval stage of TL 3.5㎜, digestive tract could be distinguished into esophagus, stomach and intestine, and the exocrine glands were appeared in the pancreas. In this stage mucosal folds, eosinophilic granule cells and brush border were observed in the posterior intestine. Yolky materials were completely absorbed and the brush border was recognized in the free surface of anterior intestine in TL 3.7㎜. In the stomach mucosal folds began to appear from TL 4.0㎜. In this time the zymogen granules were recognized in the cytoplasm of pancreatic exocrine cells. In the post-larval stage ranged from 4.5 to 5.0㎜ in TL, hepatic cords started to develop, and the mucous secretory cells of PAS positive were observed at esophagus and intestine. In the post-larval stage ranged from 6.3 to 7.0㎜ in TL, histological layer of esophagus and intestine could be distinguished into serous membrane, muscular layer, submucosal layer and mucosal layer. From over TL 9.0㎜, stomach could be distinguished into cardiac, fundic and pyloric portion, and the gastric gland began to appear at mucosal fold of fundic stomach. In the juvenile stage ranged from 10.0 to 11.0㎜ in TL, histological structures of esophagus and intestine were similar to those of adult. From over TL 15.0㎜, histological structures of stomach were similar to those of adult. Structural and functional digestive organ of black sea bream was present from the juvenile stage ranged from 15.0 to 17.0㎜ in TL.