한국에서 분리한 Reovirus의 혈구응집반응

이호왕(李鎬汪),성인화(成仁華),신종호(申宗浩) 대한바이러스학회 1978 Journal of Bacteriology and Virology Vol.8 No.1

Hemagglutinating activities of Reovirus type 2 isolated in Korea to RBC of different species of animals are summarized as follows. 1. Reovirus agglutinated human A.B.O. RBC and titer of the hemagglutinin was highest against A type RBC, but had no hemagglutinating action to RBC of rabbit, cow, guinea pig, chick and mice. 2. Most effective buffer for hemagglutination of Reovirus was the phosphate buffer and optimum pH of the buffer was 7.6.

한국형 (韓國型) 출혈열 (出血熱) - II . 병원체 (病原體) 분리 (分離)

이호왕(李鎬汪),이평우(李平佑) 대한바이러스학회 1977 Journal of Bacteriology and Virology Vol.7 No.1

Epidemic hemorrhagic fever was recognized for the first time in Korea in 1951 and since that time it has been known as Korean hemorrhagic fever (KHF). Similar diseases to KHF have been known in Manchuria, the Soviet Union, Scandinavia, Eurasia and Japan. Many attempts bave been made to isolate the causative agent of KHF and clinically similar diseases but failed. In 1976 Lee and Lee succeeded in demonstrating an antigen in the lungs of the striped field mouse, Apodemus agrarius, which gave immunofluorescent reactions with sera of patients convalescent from KHF for the first time and named it as Korea antigen. Seventy-three strains of the etiologic agent of KHF which reacted specifically only with the convalescent sera of KHF patients were isolated from lung tissues of Apodemus agrarus coreae rodents and also same agents were isolated from acute sera of two patients in adult Apodemus. The agent was successfully propagated in Apodemus through 13 passages. Experimentally inoculated mice developed sp cific fluorescent antigen in lungs, kidneys, liver, parotid glands, bladder. The agent was neutralized with convalescent serum of KHF, chloroform sensitive, passed through 0.1p millipore filter and not sensitive to antibiotics. The results indicate that the agent is the causative agent of KHF and a small virus.

한국형 (韓國型) 출혈열 (出血熱)

이호왕(李鎬汪),이평우,백락주(白樂柱),김대식(金大植),김원동(金源東),조보연(趙普衍),이명철(李明哲) 대한바이러스학회 1980 Journal of Bacteriology and Virology Vol.10 No.1

Epidemic hemorrhagic fever was recognized for the first in Korea in 1951 during Korean War among United Nations troops although similar diseases to Korean hemorrhagic fever(KHF) have been described by Japanese in China and by Russian in the Soviet Union. Since that time it has been known as Korean hemorrhagic fever and has remained endemic near the Demilitarized Zone between North and South Korea. In recent years, the disease has invaded the southern parts of the Korean peninsula and 100 to 800 hospitalized cases are clinically diagnosed each year. In 1976 Lee and Lee successfully demonstrated an antigen in the lungs of Apodemus agrarius coreae which gave specific immunofluorescent reaction with sera from patients of KHF and named it as Korea antigen. Very recently, Lee et al have reported that this antigen is the etiologic agent of KHF for the first time, and convalescent sera from hemorrhagic nephroso-nephritis in the Soveit Union, from nephropathia epidemica in Scandinavia and from epidemic hemorrhagic fever in Japan were positive for antibodies to Hantaan virus and have perfected serologic test for diagnosis of the disease. This report describes on serologic diagnosis of KHF patients and persistence of immunofluorescent antibodies to Hantaan virus after recovery of illness. 1. Immunofluorescent antibodies against Hantaan virus were appeared right after onset of fever. The highest titers were observed at 2 to 3 weeks followed by a slow decline. Antibodies also were present in each of 13 sera obtained from patients with KHF 3 to 14 years after acute disease. 2. It was possible to diagnose KHF serologically since all of the KHF patient produced antibodies against Hantaan virus by 10 days after onset of illness. 3. Occurrence of antibodies to Hantaan virus in normal Korean was 2.6% and all of the positive sera were from adults. 4. Accuracy of clinical diagnosis of KHF in suspected cases of KHF patieats at hospitals in Seoul was about 50% in compare with serologic diagnosis of the disease.

韓國에서의 Reovirus 分離

이호왕(李鎬汪),이평우,성인화(成仁華),백락주(白樂柱),김용상(金容商) 대한바이러스학회 1981 Journal of Bacteriology and Virology Vol.11 No.1

The name reovirus is sigla, short for respiratory enteric orphan, because these viruses are found in both the respiratory and enteric tracts of man but in general are orphans in the sense that they have not been associated with human disease. However, in 1973 a new reovirus was discovered which is in fast the major etiologieal agent of infantile enteritis. The genus Reovirus contains 3 mammalian serotypes which share a cammon CF antigen but are distinguishable by neutralization or HI tests. Reovirus was isolated for the first time in Korea and summary of the results are as follows: 1. Four strains of unknown virus were isolated from spleen and lymphnodes of Apodemus agrarius coreae and Microlus fortis pelliceus collected in the endemic foci of Korean hemorrhagic fever. 2. Chemieo-biologie properties of the isolated virus were; a) hemagglutination was occurred with only human red blood cells b) optimum pH for hemagglutination was 7.6 c) the nucleic acid of the virus component was RNA and d) it was insensitive to chloroform. 3. The viruses were identified as Reovirus type 2 by hemagglutination inhibition and tissue culture neutralization test using standard antisera of Reovirus type 1, 2 and 3. It was concluded that Reovirus type 2 was isolateu from rodent tissues for the first time in Korea.

서울市內에서 發生한 한국형출혈열환자 (韓國型出血熱患者)

이호왕(李鎬汪),박동호(朴東浩),백락주(白樂柱),최규식(崔奎植),황영남(黃英南),우명숙(禹明淑) 대한바이러스학회 1980 Journal of Bacteriology and Virology Vol.10 No.1

Korean hemorrhagic fever(KHF) was recognized for the first time in Korea in 1951 during Korean War among United Nations troops although similar diseases to KHF had been reported as hemorrhagic nephroso-nephritis in Russia, as epidemic hemorrhagic fever in China, as nephropathia epidemica in Scandinavia and as epidemic hemorrhagic fever in Eastern Europe and in Japan. Since that time it has been Known as Korean hemorrhagic fever(KHF) and has remained endemic near the Demilitarized Zone between North and South Korea. In recent years it appears to have spread slowly in a southwesterly direction and 100 to 800 hospitalized cases are clinically diagnosed each year. Very recently, Lee et al. Discovered the etiologic agent and animal reservoir host of Korean hemorrhagic fever and perfected serologic test for diagnosis of the disease. It has been known that KHF is an endemic disease which occuring in only certain endemic rural areas of Euro-Asia. This is the first report of occurrence of KHF patients who bad contact with house rats before illness in urban resident of Seoul and the patients were confirmed serologically. These findings cast a fresh epidemiological light on this disease, which hitherto has been regarded as rural and only rural,

韓國型 출혈열 (出血熱) Virus 의 자연계 (者然界) 宿主 Apodemus agrarius 의 혈액상 (血液像)

이호왕(李鎬汪),이평우(李平佑),성인화(成仁華),박덕수(朴德守) 대한바이러스학회 1978 Journal of Bacteriology and Virology Vol.8 No.1

Blood pictures of both normal and infected Apodemus agrarius the natural reservoir of Korean hemorrhagic fever (KHF) virus, captured in the endemic foci of Kyungido, and in non endemic areas of Chin and Jeju islands, and also infected Apodemus agrarius with KHF virus were examined and the results are summarized as follows: 1. Average nos. of RBC and WBC of normal male Apodemus agrarius were about 9,000,000 mm3 and 8,000/mm3, and of female were 8,000,000/mm3 and 7,000 mm3, respectively. 2. Differential counts of WBC of normal Apodemus agrarius were as following, lymphocyte 70-90 %, neutrophil 12-25 %, monocyte 3-10 %, eosinophil 4-6 % and basophil 0.6-0.05 %. 3, Significant increase of WBC was found at 2-3 weeks after inoculation of KHF virus into Apodernus agrarius jejudoica for the first time

候鳥에서의 日本腦炎 바이러스에 對한 中和抗體證明

이호왕(李鎬汪),강신혁(姜信赫) 대한바이러스학회 1976 Journal of Bacteriology and Virology Vol.6 No.1

Birds were found to be rescrvoir of Japanese encephalitis virus (JEV) by isolation of the virus from birds by studies of Buescher and Scherer et al. It has been thought that birds might have relationship with overwintering mechanisms of JEV and Reeves et al succeeded in inducing artificial latent infection of Western equine encephalitis virus in hirds by inoculation of the virus, There is another hypothesis to explain the every-summer endemicity of Japanese encephalitis in the endemic area of temperate zone, that migrating birds of tropical areas carry the virus to the endemic area every spring or early summer but it was not proved. This experiment was carried out to isolate Japanese encephalitis virus and also to examine the frequencies of JE virus infection in migratory birds. It was found that about 7% of migratory birds collected in springs of Korea contained neutralizing antibodies to JEV but virus isolation was unsuccessful.

韓國型 출혈열 (出血熱) 환자 (患者) 血淸內 GOT , GPT 및 LDH에 關한 硏究

이호왕(李鎬汪),성인화(成仁華),박종준(朴宗俊),분용우(憤鏞雨),원대식(元大植),김원동(金源東),조보연(趙普衍) 대한바이러스학회 1978 Journal of Bacteriology and Virology Vol.8 No.1

Korean hemorrhagic fever(KHF) reported for the first time in Korea in 1951,although in had previously been knowin both Manchuria and Russia. After the Korean War, the disease was designated as endemic in the area of the Demilitarized Zone, and since then from 100 to 800cases have been reported every year. The outbreak of patients had been localized in the vicinity of the DMZ until 1970 but since 1971 the disease has invaded the southern areas of the Korean peninsula. Lee et al' ' reported the isolation of KHF virus from the patients and Apodemus agrarius collected in the endemic areas and developed diagnosis method for serological of KHF recently In the present report, SGOT,SGPTand LDH levels of 9 KHF patients during the course of illness were studied and obtained following results. Six cases out of 9KHF patients showed increased levels of SGOT and SGPT after 2-4 weeks from onset of fever. LDH values of the sera of KHF patients were incresaed 1 week before the increase of transaminase and then decreased slowly during the course of hemorrhagic fever.

경기도 (京機道) 동두천읍 (東豆川邑) 송내리에 (松內里) 유행한 (流行) 한국형 (韓國型) 출혈열의 (出血熱) 역학적 (疫學的) 조사 (調査)

이호왕(李鎬汪),백경자(白慶子),성인화(成仁華),백락주(白樂柱) 대한바이러스학회 1979 Journal of Bacteriology and Virology Vol.9 No.1

Epidemic hemorrhagic fever with renal syndrome was recognized for the first time in Korea in 1951 among United Nation troops. Since that time it has been known as Korean hemorrhagic fever (KHF) and has remained endemic near the Demilitarized Zone between North and South Korea. In recent years the disease has invaded the southern parts of the Korean peninsula and 100 to 800 hospitalized cases are clinically diagnosed each year. Similar diseases to KHF have been described by Japanese from Manchuria, from the Soviet Union, from several countries in Eastern Europe and recently from Japan. In 1976 Lee and Lee successfully demonstrated an antigen in the lungs of the striped field mouse, Apodemus agrarius coreae, which gave sepecific immunofluorescent reaction with sera from patients convalescent from KHF and named it as Korea antigen. Very recently, Lee et al, have reported that this antigen is the etiologic agent of KHF for the first time, and convalescent sera from hemorrhagic nephroso-nephritis in the Soviet Union, from nephropathia epidemica in Scandinavia and from epidemic hemorrhagic fever in Japan were positive for antibodies to KHF virus. It is noteworthy achievement to cite that Lees method o#f serologic diag#nosis of KHF has made s#tudy o#f sero-epidemiology o#f KHF and related diseases possible. The report describes a localized outbreak of KHF in Songnaeri, Tongduchun, Kyunggido in 1971 and results of sero-epidemiologic study of the epidemic for the first time. There were 9 patients with typical clinical symptoms of KHF among 1,171 residents of Songnaeri during the outbreak of KHF from October 1,1971 to end of November 1971 and attack rate was 0.8%. Eight patients out of 9 cases were farmers in age group of over 20, and male and female attack rates were 0.8% and 0.7%, respectively, It was demonstrated for the first time that all of the patients had contained significant amounts of antibodies to KHF virus in the sera after 5 years from KHF. There was no reinfection of KHF among the persons who recovered from illness.

韓國人의 肝炎B抗原分布 關한 硏究

이호왕(李鎬汪),이용주(李容周),이평우(李平佑),백승용(白承龍),이규진(李圭振) 대한바이러스학회 1974 Journal of Bacteriology and Virology Vol.4 No.1

Acute viral hepatitis remains a rnajor worldwide public health problem. Epide'miologic and laboratory investigations indicate that 2 specific viruses are involved in this disease. The epidemiologic distinctiveness of these 2 viruses has recently been challenged. Viral hepatitis A, transrnitted primarily by the fecal-oral route, is also transmitted by the parenteral route. Viral hepatitis B occurs sporadically following parenteral inoculation of virus-infected blood products, although recent studies have deinonstrated that oral transinission can also occur. Distribution of Hepatitis B antigen (HB Ag) in Korean was investigated and the results are followings. 1. 3.1% out of 7,360 sera from paid blood donors of Korea University Woo-Suk hospital blood bank was HB Ag positive. In early stage of test, it was 5-10% but subsequently decreased gradually and was about 3% at the end of 1974. 2. The distribution of HB Ag in each age groups was investigated. The highest was in 11-20 age group (12. 7%) and 21-40 age group succeeded it. In sexual difference of it, male was twice as high as female in 11-20 age group but the difference was little in other age groups. The positive rate of antibody to HB Ag was the highest in 11-20 age group (2.7%) 3. Of 1,407 sera from out patients of Woo-Suk hospital, the distribution of HB Ag was the highest (9.8%) in the Department Gastrointestinal medicine and next was the Departments Chest surgery (9.5%) and Urology (7.8%) The distribution of hepatitis B antibody was the highest in General surgery (5.2%), Urology (4.7%) and next was Chest internal medicine (1.%) 4. The frequency of HB Ag in hlood donors from three blood banks in Seoul, positiveness was about 7% and out patients were 5.2%.