난소의 Malignant Endometrioid Tumor 3예 보고

염범우,강문자,김인선,백승룡 대한병리학회 1979 Journal of Pathology and Translational Medicine Vol.13 No.2

Lectin을 利用한 各種 組織固定液이 組織에 미치는 生化學的變化에 關한 硏究

廉範愚 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.2

The fixative is an essential for tissue processing in surgical pathology. The best fixative is that it fixes the tissue rapidly and causes the least biochemical changes in-cellular level during fixation. But all fixatives may cause some biochemical changes during fixation. The lectin is plant extract having binding activity with specific carbohydrate terminals And so the lectins are widely used in order to find out the biochemical change in neoplastic cells, fetal tissue and mitotic cells and some lectins are used as cell markers. The author made an experimental study to dissolve whether fixative might change the biochemical composition of skin, liver, kidney and spleen using with 8 lectins by ABC method. The fixatives used are Boum's solution, neutral and acid formalin, ethyl alcohol and Zenker's solution. The results are followings; 1. The epidermis of skin fixed in alcohol revealed the strongest positive reaction to PNA and Zenker's solution to PTA. 2. The renal tubular epithelial cells fixed in ethyl alcohol revealed the strongest positive reaction to PNA, DBA, Con A, SBA and RCA-Ⅰ. 3 The lymphocyte of spleen fixed in alcohol and Boum's solution showed the positive reaction to PNA.

백서 태자 조직에서 종양유전자 단백 검출에 관한 연구

염범우,이우정 고려대학교 의과대학 1996 고려대 의대 잡지 Vol.33 No.1

It has been well known that carcinogenesis begins from alteration of specific genes, such as oncogenes and suppressor genes. Although the precise mechanism is still not known, it is generally accepted that oncogenes and suppressor genes are involved not only in carcinogenesis but also in cellular differentiation and proliferation. It is important to know oncogene found in tumors is limited to the oncogenes already presented in fetal tissues. So the author carried out immunohistochemical study with c-neu, c-fos and c-jun antisera on the DDY mouse fetal tissue of 12, 14, 16 and 18 old gestational days. The results are as fol- lows. 1. c-fos protein was present in 16, 18 days old fetal immature cartilage and intestinal mucosa. 2. c-jun protein was noted only on fetal immature cartilage of 16, 18days old fetus. 3. c-neu protein was noted on the fetal liver, intestine, glial tissue skeletal muscle and pancreas after 16days old fetus.

인체 태아 및 성인 세포에서의 c-myc, c-H-ras 및 EGF 항혈청에 대한 면역조직학적 연구

염범우 고려대학교 의과대학 1994 고려대 의대 잡지 Vol.31 No.2

The exact nature of carcinogenesis is still debatable, however the discovery of oncogenes and suppressor genes encouraged the frontiers to explain the pathogenesis of malignant change. In general, the oncogenes and suppressor genes are believed to involve in cellular transformation but now it is also believed that these genes also have the role of normal cell proliferation and differentiation. So the author performed this experimental study to find out the presence of c-myc, c-H-ras and EGF protein in the liver, stomach and kidney of fetuses of variable gestational age, new born infants and adults. The results are as follows: 1. c-myc antisera revealed highest positive ratio in the liver of fetuses and newborn infant. 2. c-H-ras antisera revealed highest positive ratio in the stomach of fetuses and newborn infant. 3. EGF antisera revealed positive ratio in the liver and stomach in 9 and 5 cases.

간염, 간경변증 및 간암 조직내 림프구아형 분포에 관한 면역조직 화학적 연구

염범우 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.2

It is generally believed that the hepatic injury in viral hepatitis is due to cell mediated cytotoxicity. The natural Killer cells have defensive role on viral infection and on malignant neoplasm. In order to evaluate the role of natural killer cells in viral hepatitis, cirrhosis and hepatoma, the author performed immunohistologic study on hepatic lesions with MT1 for T Cells. LN2 for B cells and anti Leu-7 for NK cells. The results are as followings ; 1. In chronic active hepatitis, the natural killer cells are decreased in number compared with those in chronic persistent hepatitis 2. In liver cirrhosis natural killer cells are markedly decreased in number compared with those in chronic hepatitis. 3. In hepatoma, the natural killer cells are increased in unmber. Above results strongly indicated that the natural killer cells have defensive role in progress of viral hepatitis.

Epstein-Barr Virus 감염 Burkitt’s Lymphoma 세포주에 Mitomycin C 처리 시 종양유전자 발현

염범우,박설희,김민경,김철환,김인선,이대일 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.4

히스타민 및 항히스타민제가 L_(1210)백혈병세포계증식에 미치는 영향에 관한 실험적 연구

염범우,이대일,백승룡 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1

There is close relationship between growth of tumor cell and immunologic function of the host. And growth of tumor cell also partially depends on histamine which indirectly modulate degree of immunologic response of the host. However, there is no report on direct effect of histamine on the growth of tumor cell. Therefore, to evaluate direct effect of histamine on growth of tumor cell, histamine and Hz-receptor antagonist (cimetidine) were added in Fischer's culture media in concentration of 10^(-3)M , 10^(-4)M and 10^(-5)M and cultured L1:10 leukemic cell for 24,72 and 96 hours. The results are as followings; 1. Histamine iii high concentration(10^(-3)M) inhibited the proliferation of L_(1210) cell lines. 2. H2-receptor antagonist showed no inhibitory activity on the proliferation of L_(1210) leukemic cell lines a t each concentration and (10^(-3), 10^(-4) and 10^(-5)). 3. The combination of histamine and H2-receptor antagonist showed temporary inhibitory effect on the L(1210) leukemic cell lines at 24 hours culture. Above results suggest that histamine show inhibitory activity to growth of L_(1210) leukemic cell lines through H2-receptor.

정상 및 질환군에서의 전지질, Cholesterol 및 Lipoprotein分劃値의 비교검토

염범우,이대일,백승룡 대한병리학회 1978 Journal of Pathology and Translational Medicine Vol.12 No.4

胃癌細胞의 植物凝集素結合에 關한 免疫酵素組織學的 硏究

羅泳燦,廉範愚,金世民 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

To evaluate the binding reactions of various phytohemagglutinins (PHAs) on the gastric carcinoma cells, the author had undertaken an experiment using purified PHAs, such as Concanavalin A(Con-A), wheat germ agglutinin(WGA), soybean agglutinin(SBA), Dolichos biflorus agglutinin(DBA), Ulex europaeus agglutinin I (UEA I), peanut agglutinin(PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin(PTA). The tissue samples used were gastric tissue from subtotal gastrectomy or fiberoptic biopsy, diagnosed by pathologists as well differentiated adenocarcinoma and poorly differentiated adenocarcinoma signet-ring cell type adenocarcinoma, and dysplasia. The results obtained are as follows; 1. On the well-differentiated adenocarcinoma, all the PHAs except UEA I showed cytoplasmic granular reactions of the tumor cells and linear reactions along the intraluminal borders of the tumor glands. 2. On the poorly differentiated adenocarcinoma, RCA I and UEA I showed cytoplasmic reactions, and other PHAs showed variable reactions from positive to focal positive. 3. In signet-ring cell carcinoma, PNA, SBA, RCA I and UEA I showed weak reactions in the cytoplasm. 4. On the dysplastic cells, Con-A, WGA, PNA, RCA I and PTA showed cytoplasmic reactions and SBA, UEA I and DBA showed no reaction. With the above results, the binding of PHAs on the tumor cells of the stomach reveled variable patterns, depending on the degree of differentiation and the kinds of PHAs used.

타액선 암종세포의 식물응집소 수용체 분포에 관한 면역조직화학적 연구

채양석,염범우,이대일 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1

Malignant tumors of the salivary glands are variable in their histologic pictures, and their histochemical changes in membrane carbohydrate composition have not been fully understood. The author selected formalin fixed, paraffin embedded normal tissue and malignant tumors of salivary gland, and performed immunohistochemical study on the selected tissue materials by avidin biotin peroxidase complex method using lectins such as Concanavalin A (Con A), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin I (UEA I), Peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin (PTA). The results are summarized as follows: 1. Lectins showing different reaction pattern between normal and malignant tumor cells of the salivary glands were DBA, UEA-1, SBA and PNA. 2. DBA and SBA showed positive reaction with normal duct epithelium, but they did'nt react with malignant turner cells. 3. UEA-1 revealed positive reaction with normal duct epithelium and cancer cells of the mucopeidermoid carcinoma. But it did'nt react with adenoid cystic carcinoma cell and acinic cell carcinoma cell. 4. PNA showed positive reaction with mucoepidermoid carcinoma cell and negative reaction with nor-mal duct epithelium, adenoid cystic carcinoma and acinic cell carcinoma cell. Above findings suggest loss of N-acetyl-galactosaminyl moiety in malignant tumors of salivary gland, loss of L-fucosyl in adeniod cystic cacinoma and acinic cell carcinoma, which are present on normal duct epithelium, and appearance ofβ-D-galactosyl moiety in mucoepidermoid carcinoma which is absent on normal duct epithelium.