월남어의 음성 구조와 기본구문

梁熙榮 陸軍士官學校 1972 한국군사학논집 Vol.10 No.-

2003-2011년의 한 삼차 의료 기관 내 비결핵 항산균 분리 추세

양희영 고신대학교 의과대학 2011 고신대학교 의과대학 학술지 Vol.26 No.2

OBJECTIVES: This study was performed to investigate the prevalence of nontuberculous mycobacteria (NTM) species and to determine the clinical significance of NTM isolates. METHODS: From January 2003 to July 2011, NTMs were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or reverse blot hybridization assay (REBA). And pulmonary NTM infection was diagnosed by clinical history, underlying disease, radiological and microbiological findings according to the diagnostic criteria of 2007 American Thoracic Society (ATS). RESULTS: Of the 697 AFB culture-positive specimens, NTM was detected in 149 (21.4%) specimens. Among 154 NTM isolates from 149 specimens, M. avium-intracellulare complex (MAC) (48.1%) was the most frequently isolated organisms followed by M. abscessus (13.6%), M. gordonae (9.1%), M. kansasii (8.4%), M. szulgai (3.9%), M. fortuitum complex (3.3%), M. scrofulaceum (2.0%), M. malmoense (1.3%), M. chelonae (1.3%), M. marinum (1.3%), M. genavense (1.3%), M. lentiflavum (1.3%) and M. mucogenicum (0.6%). Among 147NTM isolates from 142 respiratory specimens, 54 NTM isolates (36.7%) were causative organisms in NTM pulmonary infection. CONCLUSIONS: The isolation rate of NTM was 21.4% in clinical specimen, and in some cases NTM species results in pulmonary NTM infection. Because the treatment of pulmonary NTM infection depends on the infecting species, accurate identification and clinical significance of NTM are required for adequate treatment.

20세기후반 이후 패션 구성방식에 나타난 모듈러 시스템 -욜리 탱, 존 리베, 갈야 로젠필드의 작품을 중심으로

양희영 한국의류학회 2007 한국의류학회 학술대회논문집 Vol.2007 No.1

Altered expression of membrane proteins in colorectal cancer patients

양희영,최훈인,이태훈 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8

내가 걸어 온 어둠과 빛의 세계

양희영 새가정사 1964 새가정 Vol.11 No.9

The Oxidative Modification of COL6A1 in Membrane Proteins of Ovarian Cancer Patients

양희영,이태훈 사단법인 한국동물생명공학회 2012 Reproductive & developmental biology Vol.36 No.1

Ovarian cancer is the most lethal gynecological malignancy, and specific biomarkers are important needed to improve diagnosis, prognosis, and to forecast and monitor treatment efficiency. There are a lot of pathological factors,including reactive oxygen species (ROS), involved in the process of cancer initiation and progression. The oxidative modification of proteins by ROS is implicated in the etiology or progression of disorders and diseases. In this study,a labeling experiment with the thiol-modifying reagent biotinylated iodoacetamide (BIAM) revealed that a variety of proteins were differentially oxidized between normal and tumor tissues of ovarian cancer patients. To identify cysteine oxidation-sensitive proteins in ovarian cancer patients, we performed comparative analysis by nano-UPLC-MSE shotgun proteomics. We found oxidation-sensitive 22 proteins from 41 peptides containing cysteine oxidation. Using Ingenuity program, these proteins identified were established with canonical network related to cytoskeletal network,cellular organization and maintenance, and metabolism. Among oxidation-sensitive proteins, the modification pattern of Collagen alpha-1(VI) chain (COL6A1) was firstly confirmed between normal and tumor tissues of patients by 2-DE western blotting. This result suggested that COL6A1 might have cysteine oxidative modification in tumor tissue of ovarian cancer patients.

Intrapulpal Temperature Change during Cavity Preparation on the Enamel and Dentin with an Er:YAG Laser

양희영,김미은,김기석,Yang, Hee-Young,Kim, Mee-Eun,Kim, Ki-Suk Korean Academy of Orofacial Pain and Oral Medicine 2005 Journal of Oral Medicine and Pain Vol.30 No.4

The purpose of our study was to investigate whether the intrapulpal temperature during cavity preparation of enamel or dentin with Er:YAG laser still remained in range of safety for dental pulp protection when combined with appropriate water flow rate. The effect of different pulse repetition rates at the same pulse energy during ablation was evaluated as well. Caries-free, restoration-free extracted human molar teeth were prepared for the specimen and divided two experimental groups of enamel and dentin. Each group comprised 5 specimens and each of tooth specimens were embedded into a resin block each and measuring probe was placed on the irradiated pulpal walls. For experiments of dentin ablation, enamel layers were prepared to produce dentin specimen with a same dentin thickness of 2 mm. A pulse energy of Er:YAG laser was set to 300 mJ and three different pulse repetition rates of 20 Hz, 15 Hz and 10 Hz were employed. Laser beam was delivered with 3 seconds and less per application over enamel and dentin surfaces constant sized by $3\;mm{\times}2\;mm$ and water spray added during irradiation was a rate of 1.6 ml/min. Temperature change induced by Er:YAG laser irradiation was monitored and recorded While enamel was ablated, there was no significant difference of temperature related to pulse repetition rates(p=0.358) and temperature change at any pulse repetition rate was negligible. Significant statistical difference in temperature changes during cavity preparation in dentin existed among three different pulse groups(p=0.001). While temperature rise was noticeable when the dentinal wall was perforated, actual change of temperature due to Er:YAG laser irradiation was not enough to compromise safety of dental pulp when irradiation was conjugated with appropriate water spray. Conclusively, it can be said that cavity preparation on enamel or dentin with an Er:YAG laser is performed safely without pulp damage if appropriate volume of water is sprayed properly over the irradiated site.

Proteomic Analysis of the Increased Proteins in Peroxiredoxin Ⅱ Deficient RBCs

양희영,이태훈 사단법인 한국동물생명공학회 2012 Reproductive & developmental biology Vol.36 No.1

Peroxiredoxin Ⅱ (Prdx Ⅱ; a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Prdx Ⅱ has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in a variety of disease states associated with oxidative stress, including cancer and aging-associated pathologies. But, the precise mechanism is still obscure in various aspects of aging containing ovarian aging. Identification and relative quantification of the increased proteins affected by Prdx Ⅱ deficiency may help identify novel signaling mechanisms that are important for oxidative stress-related diseases. To identify the increased proteins in Prdx Ⅱ—/— mice, we performed RBC comparative proteome analysis in membrane fraction and cytosolic fractions by nano-UPLC-MSE shotgun proteomics. We found the increased 86 proteins in membrane (32 proteins) and cytosolic (54 proteins) fractions, and analyzed comparative expression pattern in healthy RBCs of Prdx Ⅱ+/+ mice, healthy RBCs of Prdx Ⅱ—/— mice, and abnormal RBCs of Prdx Ⅱ—/— mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cellular morphology and assembly, cell-cell interaction, metabolism, and stress-induced signaling. Moreover, protein networks among the increased proteins were analyzed to associate with various diseases. Taken together, RBC proteome may provide clues to understand the clue about redox-imbalanced diseases.

한국인 턱관절장애 환자의 유병률과 진료 양태

양희영,김미은,Yang, Hee-Young,Kim, Mee-Eun 대한안면통증구강내과학회 2009 Journal of Oral Medicine and Pain Vol.34 No.1

While previous epidemiological studies on temporomandibular disorders (TMD) have been based on a given health center or population sample, no study has been performed on general population of Korea, especially concerning about treatment pattern such as clinician’s specialty involved in TMD treatment, types and amount of prescription medication and cost. This study aimed to investigate magnitude of health visits and treatment patterns for Korean patients with TMD through the computerized database of Health Insurance Review and Assessment Service (HIRAS). Inclusion criteria were all patients registered on the HIRAS database over 3 years' period from 2003 to 2005 and the medical records of patients with TMD as a main diagnosis were extracted. Information collected was as follows; distribution related to gender, age and region and type of hospital the patients visited, treatment duration, clinicians' specialty involved in treatment, cost, types of prescription medication and surgical treatment. The results of this study indicated that 0.15% of the population yearly sought TMD treatment, presenting with increase of incidence over the three years. Most of TMD patients were women (99.8%) and the biggest age group was second and third decades and decreased with age. Seoul and Kyeonggi province presented with higher incidence of TMD compared to the other regions of Korea, which seems to be related with magnitude of population. 56% of TMD patients visited primary care sector and the numbers of treatment visits was the highest in dental clinic (38.4%), followed by orthopedics (28%) and ENT (13.6%) clinics in order. Duration of prescription medication was the longest for anti-inflammatory analgesics, followed by antipsychotic drugs and muscle relaxants. Inpatient care related to TMD was primarily performed in dental hospital compared to medical hospital. Medical database of HIRAS provided comprehensive and vast information on epidemiologic characteristics and treatment patterns for patients seeking TMD treatment, which can be more reliable data to expect medical demand for TMD in condition that accurate diagnosis and standardized treatment is delivered in clinical settings.

결핵 진단을 위한 실시간 중합효소연쇄반응의 유용성 평가

양희영 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: The polymerase chain reaction (PCR) test has been used for rapid, sensitive and specific detection of Mycobacterium tuberculosis (MTB). Recently, real-time PCR has been introduced as a rapid and accurate assay but has not yet been fully evaluated its reliability for diagnosis of tuberculosis. We compared the recently developed real-time PCR COBAS TaqMan MTB assay (Roche Molecular Systems, USA) and AdvanSure TB/NTM real-time PCR (LG life Science Diagnostic Division, Korea)) with COBAS Amplicor MTB PCR assay (Roche Molecular Systems, USA) by PCR-hybridization and conventional acid fast bacilli (AFB) smear and MTB culture for diagnosis of tuberculosis. Methods: A total of 91 specimens were tested by using COBAS Amplicor MTB PCR, COBAS TaqMan MTB assay, AdvanSure TB/NTM kit, acid fast bacilli (AFB) smear and MTB culture.The sensitivity, specificity and positive and negative predictive values of the five methods were evaluated according to final clinical diagnosis result. Results: In 91 cases, 18 cases were diagnosed with tuberculosis based on the clinical diagnosis. The sensitivity, specificity and positive and negative predictive values of each method were 88.9%, 97.2%, 88.9% and 97.3%, respectively, for COBAS Amplicor MTB PCR, 83.3%, 95.9%, 83.3% and 95.9% for COBAS TaqMan MTB assay, 88.9%, 94.5%, 80.0% and 97.2% for AdvanSure TB/NTM kit, 72.2%, 100%, 100% and 93.6% for AFB smear and 88.9%, 100%, 100% and 97.3% for MTB culture. Conclusion: Real-time PCR was comparable to PCR-hybridization and conventional method and was a reliable method for an early diagnosis of tuberculosis in clinical laboratories.