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羅圭煥 한국환경독성학회 1986 환경독성보건학회지 Vol.1 No.1
1985년 10월 2일에 도시형 하천인 원주천의 저질을 각 용매로 추출한 유기물질에 대하여 혈액독인 methemoglobin 생성능을 측정하였다. 공장폐수의 영향을 받는 St. C 및 St. D수역의 저질에서는 n-hexane 추출물에 methemoglobin 생성능이 뚜렷하였으나 도시하수의 영향이 심한 St. A의 각용매추출물에서도 methemoglobin 생성능이 인정 되었다. 각 유기용매 추출물의 methemoglobin을 20% 생성하는 추출물농도 범위는 n-hexane 추출물이 0.095~0.28mg/ml이었으며 methanol 추출물은 0.85~1.3mg/ml ethylacetate 추출물은 1.95~2.80mg/ml이었다. The writer measured methemoglobin formation with solvent (n-hexane, ethylacetate and methanol) organic extracts from the sediments of the Wonju stream run through Wonju city on Octover 2nd, 1985. Hemoglobin was converted to methemoglobin with the n-hexane extract obtained from the sediment of the St. C and St. D was polluted industrial wastewater, but not with that from the St. B was polluted with the urban wastewater. The formation of methemoglobin was remarkable with the all solvent extracts from the sediment of the uper stream of the Wonju stream. Doses of the solvent extracts for 20% methemoglobin formation in the researched Wonju streams were as follows; 0.095~0.28mg/ml in n-hexane extract. 0.85~1.3mg/ml in methanol extract and 1.95~2.80mg/ml in ethylacetate extract.
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나규환,이장훈 환경독성보건학회 1992 환경독성보건학회지 Vol.7 No.3
Physical compositions of solid waste in Wonju, shopping area were investigated with combustibles 78.13% (papers 35.89%, foods 14.41% etc.) incombustibles 21.87% (glass and ceramics 11.02%, metals 6.0% etc.) in 1991, solid waste in apartment area were investigated with combustibles 84.27% (foods 34.29%, papers 22.58% etc.), incombustibles 15.73% (glass and ceramics 8.77%, metals 4.85% etc.) and residence area were characterised with combustibles 70.37% (foods 33.55% , papers 10.53% etc.) and incombustibles 29.63% (ash of briquet 17.29%, glass and ceramics 7.49% etc.). Water qualities of a leachate from municipal landfill of Wonju city were analysed pH 8.0~8.4, total suspended solid 102~140 mg/1, CN￣ 0.003~ 0.008 mg/1, NO$_2$-N 0.108~0.294 mg/1 and phenols 0.46~1.12 mg/1. Volume of the leachate for 20% methemoglobin formation were 0.2~0.4 mg/ml in Octever, 0.3~0.4 ml/ml in December sampling, 0.2~0.3 ml/ml in St.1 and 0.4 ml/ml in St.3.
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Isolation and Characterization of Trophoblast Stem Cells-Like Cells Derived from Human Term Placenta
나규환,최종호,차동현,김기진,신경선 한국발생생물학회 2010 발생과 생식 Vol.14 No.3
The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta (≥38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, our results support an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.
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