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RISS 인기검색어
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- 저자 : 홍성회(Sung Hoi Hong) (검색결과 4 건)
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벼의 arginine decarboxylase DNA clone 의 재조합 및 염기서열 분석
홍성회(Sung Hoi Hong),신정섭(Jeong Sheop Shin),정지웅(Ji Ung Jeung),옥승한(Sung Han Ok) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2
Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 by of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector anti the short 500 by Pst1 digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted .amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47%n and 38% were previously reported in oat and E. coli, tomato ;md oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that ADC is expressed as a transcript of approximately 2.5 kbp in the rice seedling leaf tissues.
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HLA-B27 DNA Typing using Group Specific Polymerase Chain Reaction
Kyung-Ok Lee(이경옥),Sung-Hoi Hong(홍성회),Moon-Ju Oh(오문주),Kyung-In Kim(김경인),Min-Jung Kim(김민정) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.2
HLA-class Ⅰ 항원의 HLA-B 유전자좌에 존재하는 HLA-B27 유전자는 임상적으로 강직성 척수염과 강한 관련성이 있음이 보고되고 있으며, 현재 HLA 유전자중 질병과의 관련성을 보기 위한 검사로 임상에서 가장 널리 사용되고 있다. 대부분의 검사실에서는 현재까지 혈청학적 검사방법을 이용하여 HLA-B27 검사를 실시하고 있는데, 이 방법은 시약이 고가이고, 검체의 안정성과 보관이 어려우며, 분석시간이 오래 걸리는 등 불편한 점이 있고, 또한 현재에도 계속 새로운 HLA-B27 대립유전자가 발견되고 있으므로 위음성의 가능성도 배제할 수 없어, 보다 정확한 검사방법이 요구되고 있다. 최근 HLA-B27 대립유전자의 염기배열이 대부분 밝혀져 혈청학적 방법 대신 DNA를 이용한 typing 방법이 보고되고 있다. 저자들은 HLA-B27 대립유전자에 공통으로 존재하는 염기배열 부분을 선택하여 group specific PCR (Polymerase Chain Reaction)을 실시하고 그 유용성을 검토하였다. 혈청학적 방법으로 HLA B-27 형이 확인된 검체 56 개와 4 개의 표준세포주 (HOM-2, JESTHOM, WT24, BTB)를 이용하여 혈청학적 방법과 DNA typing을 비교한 결과, 두 방법사이에 완벽한 일치를 나타내었다. 따라서 group specific PCR을 이용한 HLA-B27 DNA typing은 검체 및 시약의 안정성이 높고, 경제적이며 신속한 검사가 가능하므로 임상에서 활용성이 매우 클 것으로 사료된다. HLA-B27 gene, one of the HLA-class Ⅰ molecule, is strongly associated with ankylosing spondylitis. It has been most frequently used as a disease-correlated HLA gene by clinicians. In most laboratories, conventional HLA-B27 typing is still performed by cell cytotoxicity tests or fluorescence serology with specific antibodies. In this study, DNA typing method for HLA-B27 was developed by using group specific Polymerase Chain Reaction (PCR). Four HLA-B27 cell lines (HOM-2, JESTHOM, WT24 and BTB) and fifty six B27 Korean individuals defined by serology were used. The results of control cell and B-27 positive individual samples were correlated well with the data which was performed by serological method. All of B27 positive PCR products gave positive signals on Southern blot hybridization with B27 specific probe. This study shows that the HLA-B27 DNA typing is a relatively simple, fast and practical tool for the determination of the HLA-B27 gene in routine clinical laboratory work.
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손주용(Joo Yong Sohn),반성철(Sung Chul Ban),신정섭(Jeong Sheop Shin),홍성회(Sung Hoi Hong) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.3
This experiment was conducted to characterize and quantity the free sugars (glucose, fructose, sucrose. maltose) contained in many different portions of watermelon (Citrullus vulgaris L.) and muskmelon (Cucumis melo var. reticulatus Naud.) fruits by High Performance Liquid Chromatography (HPLC). Free sugars were mainly fructose, glucose, sucrose, and their contents were variable among portions. Total free sugar contents were higher in the stylar end and side than in the stem end of both watermelon and muskmelon. Total free sugar contents increased from the periphery toward the central core in watermelon and except central core content seeds in muskmelon. Ratio of nonreducing to reducing sugars [(fructose+glucose)/sucrose] was gradually decreased from the periphery toward the middle area in watermelon, though the central core showed higher value than the middle area. For the edible portion of muskmelon, the ratio was decreased toward middle area, and no significant difference was observed between the central core and the middle area. However, reducing sugars and nonreducing sugar were all increased from the periphery toward the central core in watermelon. In contrast with watermelon, reducing sugars were decreased in muskmelon.
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