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해녀콩의 유식물 (幼植物) 자엽내 BAρNAase 의 성질
고석찬(Suck Chan Koh),허인옥(In Ok Heo),권영명(Young Myung Kwon) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.2
N-α-benzoyl-DL-arginine ρ-nitroanilide hydrolase (BAρNAase) was partially purified and characterized from the cotyledons of Canavalia lineata. The overall purification and final recovery were 77.5-fold and 7%, respectively. The apparent mol wt of the native enzyme was estimated as 200 kD from the gel filtration and this enzyme was revealed as one of the gelatin-hydrolyzed proteases by activity staining with gelatin-containing gel. The optimum pH for the hydrolysis of N-α-benzoyl-DL-arginine ρ-nitroanilide (BAρNAase) was about 9.5. This enzyme catalyzed the hydolysis of BAρNA with Vmax of 15.5 unit/mg and Km value of 1.6 mM which were different from them of bovine-pancreatic trypsin. The catalytic activity of this enzyme was strongly inhibited by phenylmethanesulfonyl fluoride (PMSF) but insensitive to aprotinin, indicating that it was a serine proteinase but not a trypsin-like one. And this enzyme was slightly stimulated by Ca^2+ and mg^2+ but remarkably inhibited by Mn^2+, HG^2+ and Zn^2+.
Agrobacterium rhizogenes를 이용한 까마중의 형질전환과 모상근 배양
고경수(Kyung Soo Ko),허인옥(In Ok Heo),양관팔(Kwan Pal Yang),이운진(Woon Jin Lee),김창민(Chang Min Kim),조필형(Pill Hyeong Jo) 한국생약학회 1991 생약학회지 Vol.22 No.1
The study aimed to confirm transformation, morphology, steroid alkaloidal TLC pattern and growth rate of hairy roots. The Solanum nigrum plantlets were inoculated with Agrobacterium rhixogenes strain 15834. Hairy roots were induced by plasmid. Agropine and mannopine were detected in the hairy roots. The organization of hairy roots on the transectional morphology was undifferentiated. Culture on the medium containing hormone(IBA 2, kinetin 0.1 ㎎/ℓ) altered hairy roots into callus. The growth rate of hairy roots on the NN30 liquid medium was 52 times heavier than in the original state and this was higher than on the other media. The results of TLC analysis indicated that the hairy roots produced steroidal alkaloids resembling those of normal roots.