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허로운,박재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
The exact cause of Rheumatoid arthritis (RA) is not known, but activated macrophages are considered to be the major cell contributing to joint inflammation and pathogenesis of joint damage, common in RA patients. As a result, targeting activated macrophages has been considered as a powerful way to achieve effective RA therapy. In this study, we prepared self-assembled dextran sulfate nanoparticles (DSNP) to target methotrexate (MTX) to inflammatory joints of RA. DSNPs showed selective internalization into the activated macrophages possibly via scavenger receptor class A-mediated endocytosis. When systemically administered into mice, the DSNPs substantially accumulated in inflamed joints, implying their high targetability to RA tissues. Additionally, the MTX-loaded DSNPs demonstrated significantly improved therapeutic efficacy compared to free MTX alone. Overall, it was evident that the DSNPs have a great potential as therapeutic agent carriers for RA imaging and therapy.
Gold-decorated nanocomplexes as biostable siRNA carrier for improved in vivo tumor targeting
허로운,박재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
The major issues, associated with non-viral vectors for small interfering RNAs(siRNAs) are their poor stability and lack of tumor targetability in vivo. In this study, we developed gold-decorated polyethyleneimine (PEI)/siRNA complexes with a corona of PEGylated hyaluronic acid (GDCs). When GDCs were exposed to 50% serum, no significant change in the structural integrity of siRNA was observed at least for 24 h, during which free siRNAs completely degraded. Both GUCs and gold-undecorated (PEI)/siRNA complexes (GUCs) were efficiently internalized into CD44+ melanoma cells (B16F10), but were rarely taken up by CD44- normal fibroblast cells (NIH3T3). For Red fluorescence protein (RFP)-expressing B16F10 cells, GDCs showed the highest gene silencing efficacy, compared to GUCs and free siRNA. Based on their high stability and tumor targetability, GDCs might have promising potential as the siRNA delivery system for cancer therapy.