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한우의 정상 난포와 난포낭종 난포에서 Aquaporin7 발현 양상
김창운,한신규,최창용,Kim, Chang-Woon,Han, Sunkyu,Choe, Changyong 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.1
Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen ($10{\mu}g/ml$) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary.
신용석 ( Shin Young-suk ),한신규 ( Han Sin-gyu ) 한국구조물진단유지관리공학회 2004 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.8 No.1
There in no subjective criterion of tunnel safety inspection and diagnosis. Due to that reason, many engineers are confused in determining degree of state evaluation. And therefore, there is a strong demand to accomplish a rational and objective criterion for tunnel safety inspection and diagnosis. In this study, the procedure of objective state evaluation that is borrowed from "The Guide Line for Tunnel Safety Inspection and Diagnosis", which was published by Ministry of construction & transportation and KISTEC, is suggested and explained well.
[제1분과/비파괴검사 및 계측기술] 터널의 객관적인 상태평가 기준정립
신용석(Shin yong-Suk),한신규(Han sin-gyu) 한국구조물진단유지관리학회 2004 한국구조물진단학회 학술발표회논문집 Vol.- No.-
There in no subjective criterion of tunnel safety inspection and diagnosis. Due to that reason, many engineers are confused in determining degree of state evaluation. And therefore, there is a strong demand to accomplish a rational and objective criterion for tunnel safety inspection and diagnosis. In this study, the procedure of objective state evaluation that is borrowed from ""The Guide Line for Tunnel Safety Inspection and Diagnosis"", which was published by Ministry of construction & transportation and KISTEC, is suggested and explained well.