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Strain Improvement for High Gentamicin Production Using Micromonosporas purpurea
최두복,신대윤,Choi DuBok,Yin Pemin,Choi On You,Shin Dae-Yewn Korean Society of Environmental Health 2005 한국환경보건학회지 Vol.31 No.4
Micromonosporas purpurea로부터 효율적 gentamicin 생산을 위해 protoplast fusion와 protoplast mutagenesis 방법이 검토 되었다. $CO^{60}\;irradiation\;(2.3{\times}10^5$ units, UV 3 min) 방법에 의해서 MP3-112, MP3-141, MP3-143을 분리 했다. 특히 MP3-143균주는 최대 gentamicin생산량이 얻어졌다. 개량된 MP3-143균주를 이용해서 탄소원 소비, 균체성장, 그리고 gentamicin 생산량이 batch culture에서 비교되었다. MP3-413와 parent 균주의 glucose 소비는 배양 2일과 3일 후에 각각 완전히 이루어졌다. 그러나 균체성장과 Soybean oil 소비는 비슷한 결과 얻어졌다. Gentamicin최대 생산량은 배양 5일 후 29756 U/ml였다. 이 결과는 parent 균주에 비해 생산량이 5.6배 증가했다.
Saccharification from Alginate using Pseudoalteromonas agarovorans
최두복(DUBOK CHOI),조훈(HOON CHO) 대한환경위생공학회 2008 대한환경위생공학회 정기총회 및 학술발표회 Vol.- No.-
For efficient saccharification of alginate, marine bacteria was isolated from seawater near the Korean south coast. Based on 16S rDNA sequence, the isolated strain was identified as Pseudoalteromonas agarovorans. Various environmental factors affecting saccharification of alginate using Pseudoalteromonas agarovorans CHO-12 have been investigated in flask cultures. The saccharification was found to be optimal at the temperature of 29℃ and pH of 8.0. Among various NaCl concentrations, the maximum sugar concentration was obtained at 13.8g/l when 30g/l of NaCl was used. However, when NaCl was below 10g/l, sugar concentration decreased remarkably. Yeast extract and CSL were the best nitrogen source for efficient saccharification. The maximum saccharification rate at 50l of reactor was obtained, 15 g/l after 2 day of culture, which was about 5.0 fold times higher than that of S. maltophilia.
최두복(DuBok Choi),조훈(Hoon Cho) 대한환경위생공학회 2009 대한환경위생공학회 정기총회 및 학술발표회 Vol.2009 No.-
This study was carried out to investigate effect or extraction process on the physico-chemical properties from seed oil of wild Camellia sinensis grown in Iksan. When methanol was used a extract solvent, the polyphenol compound concentration was highest, 424.7㎎/g, which was 2.5 fold higher than other solvents. Especially, when the methanol concentration was increased from 20 to 80%, the polyphenol concentration was relatively from 40 to 100%. The specific gravity and refractive index of green tea see oil (GTSO) were 0.92g/㎤ and 1.45, respectively. The chromaticities of light, red, and yellow in CRO were 88.6 and 98.7, respectively. Among various fatty acids, Oleic acid (C18:1) concentration was highest, 49.3% and Linoleic acid (C18:2) concentration was 25.8%. The degree of expediting rancidity of CRO was an order of Fe²?> Cu²?> Cr²?> Zn²?> Ni²?>. Especially, when Cu²?and Fe²? was used, the peroxide value concentration was about 4.0 fold higher than non addition of them. The inhibition effect of rancidity of GSTO using antioxidant with Cu²?and Fe²? was increased.
이명자(Lee Myung-ja);최두복(Dubok Choi);이희경(Lee Hee-kyung) 국제차문화학회 2021 차문화ㆍ산업학 Vol.52 No.-
본 연구는 전라북도 익산 웅포에서 채취한 찻잎을 이용해서 발효한 차가 미용 화장품 소재로서 가능성이 있는지 검토하기 위해 여러 추출물에 대한 세포독성, 아질산 제거 효과, 티로신아제 활성, 엘라스타아제 활성, 및 콜라겐아제 활성을 각각 분석하였다. 발효차는 유기용매에 의한 추출물의 종류와 관계없이 세포독성이 100, 300 및 500μg/mL 범위의 농도에서도 전혀 존재하지 않았다. 티로신아제 저해 활성은 대조군인 ascorbic acid보다 저해 활성이 낮았지만 여러 추출 중 에탄올 추출물이 가장 우수효과가 나타났다. 콜라겐아제 및 엘라스타아제 저해 효과를 검토한 결과 발효차의 에탄올 및 열수 추출물이 농도 의존적으로 저해 활성이 증가하였다. 따라서 발효차 열수 및 에탄올 추출물은 농도가 증가함에 따라 피부 미용효과도 증가하였다. 특히, 여러 추출 중에서 에탄올 추출물은 미백 및 주름 개선 효과가 다른 추출물보다 높아 기능성 화장품 소재로서 가능성을 나타내었다. 에탄올 및 열수 추출물은 화장품제조 분야에서 사용되는 일반적인 유기용매를 이용한 추출 방법에 비교하면 안전성이 매우 높고 또한 피부에 자극이 낮기 때문에 기능성 화장품 소재로 사용 가능성이 있다고 생각한다. 그러나 미용 화장품 소재로서의 사용하기 위해서는 구체적인 임상에 대한 연구개발이 필요하다. This study analyzed cytotoxicity, tyrosinase, collagenase, and elastase activities using various extracts to examine whether the tea fermented from tea leaves collected from Ungpo, Iksan-si, Jeollabuk-do, could be a cosmetic material for beauty. Fermented tea did not exist at all at concentrations in the 100, 300 and 500μg/mL ranges, regardless of the type of extracts. Tyrosinase inhibitory activity was less active than the ascorbic acid as the positive control group. However, among several extracts, the best effect was shown in ethanol extract. The results of reviewing collagenase and elastase inhibitory effects showed that ethanol and hot water extracts of fermented tea were compared to controls, resulting in concentration-dependent low activity increase. Therefore, hot water and ethanol extractions using fermented tea also increased the skin beauty effects as concentrations increased. In particular, ethanol extract showed higher whitening and wrinkle improvement effects than other extracts, indicating their potential as functional cosmetic materials. Ethanol and hot water extracts of fermented tea are highly likely to be used because they are very safe and less irritating to the skin compared to general organic solvents used in cosmetics manufacturing. However, the specific clinical research and development are needed to use it as a cosmetic material for beauty.
증숙 더덕 용매별 추출물의 Nitric Oxide 생성 저해 효과 및 Acetylcholinesterase 저해활성
최현숙,최두복,Choi, Hyun-Suk,Choi, DuBok 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.3
Steaming is a method that has traditionally been used for medicinal plant extraction. This study investigated nitrite oxide production, ferrous ion chelating activity, α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities of ethanol, acetone and hot-water extracts of Codonopsis lanceolata prepared by steaming seven times. MTT assay showed that each extract was non-toxic up to a concentration of 700 ㎍/mL confirming that there was no cytotoxicity in all extracts. The α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities exhibited by the hot-water extract obtained from steaming seven times were higher (83.1%) than the other extracts. Higher production of nitrite oxide and better ferrous chelating activity was recorded with hot-water extract compared to ethanol and acetone extracts. These results indicated that more steaming of Codonopsis lanceolata extracts would be required to validate the possibility of developing antioxidants. Also, further study is needed to determine if the components present in the tested extracts might be useful in the prevention of Alzheimer's disease. These results showed that hot-water extracts may be useful for their antioxidant and the production inhibitory activity of nitrite oxide. It will be helpful in the investigation of the constituent analysis of the steam-processed product of Codonopsis lanceolata.