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Hitachi 7600 p-모률을 이용한 유리형경쇄 정량검사의 항원과잉역 반응
차경호 ( Kyong Ho Cha ),김승희 ( Sung Hεe Kim ),송창은 ( Chang Un Song ),심양보 ( Yang Bo Sim ),채효진 ( Hyo Jin Chae ) 대한임상검사과학회 2009 대한임상검사과학회지(KJCLS) Vol.41 No.4
The analysis of serum free light chains (sFLCs) can improve the diagnosis and monitoring of multiple myeloma and other plasma cell dyscrasias. As with other immunoassays, sFLCstests are subject to potential antigen excess and heterophilic antibody interference. We describe 9 cases of sFLCs antigen excεss in patients with multiple myeloma using the FreeliteTM Human Kappa and Lambda Frεe Kits (Thε Binding Site ltd., Birmingham, UK) and the Hitachi7600 P module turbidimetric system. A total of 1,247 consecutive samples from 250 patients with multiple myeloma were assayed for sFLCs from April to September, 2009. The samples were assayed using an initial dilution of 1 :5and subsequent dilutions of 1 :50 and 1: 100. The same samples were analyzed for the presence of monoclonal gammopathies using serum protεin electrophoresis (SPE) and immunofixation electrophoresis (IFE). There were 9 samples (0.72%) of antigεn excess with 3 cases of kappa (0.24%) and 6 cases of lambda (0.48%). Thεse cases reprεsents an example of antigen excess or " hook effect" using the sεrum free light chain assays and mandates high level of attεntion to falsely low sFLC 1εvels due to antigen excess, especially when it is disaccordant to other assay rεsults or c1inical manifestations.
LNG 탱크 컨테이너의 내부압력 변화 분석 및 실제 홀딩타임 측정
류영돈(Young-Don Ryou),이진한(Jin-Han Lee),조영도(Young-Do Jo),오영삼(Young-Sam Oh),차경호(Kyong-Ho Cha) 한국가스학회 2013 한국가스학회지 Vol.17 No.6
이 논문에서는 특례기준에 따라 국내에서 최초로 제작하고 검사한 LNG 탱크 컨테이너를 이용하여 육상 및 해상 운송 시연사업을 하고, 탱크 컨테이너를 운송할 때 탱크 컨테이너 내부에 충전된 LNG의 압력변화와 탱크 컨테이너의 실제 압력유지기간을 측정하였다. 탱크 컨테이너 내부의 압력은 충전 직후에는 급속하게 상승하고, 탱크 컨테이너를 이동할 때 급속히 하강하나, 일정시간이 경과한 후에는 기액평형상태 그래프의 온도-압력 변화와 일치함을 확인하였다. 또한, 탱크 컨테이너의 실제 압력유지기간은 특례기준에서 규정한 20일 이상을 만족함을 확인하였다. Internal pressure variation analysis and actual holding time test on ISO LNG tank containers which were made in Korea for the first time according to the special notification of ISO tank container manufacture have been conducted during the transport demonstration projects of the tank containers by tractor, train and ship. The internal pressure of the LNG tank container increased rapidly after LNG filling and dropped during moving the container. However, it was stabilized as time passed and followed the liquid-vapor equilibrium graph. In addition, actual holding time of the tank container was more than 20 days which was satisfied with the special notification of LNG tank container manufacture.