Polymerase chain reaction을 이용한 독소생산성 Pasteurella multocida의 검출

지영철,이동석,한정희,한경수,한태욱,Chi, Yongzhe,Lee, Dong-seok,Han, Jeong-hee,Han, Kyung-soo,Hahn, Tae-wook 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.1

Pasteurella multocida is kind of commensal bacteria in the upper respiratory tract of pigs. It is classified toxigenic and nontoxigenic strains based on the production of dermonecrotic toxin. Toxigenic strain is most associated with atrophic rhinitis which brings great economical loss in swine industry. However, toxigenic and nontoxigenic strains do not differ by diagnostic biochemical reaction or morphology. One of recently developed techniques, PCR detects the toxigenic P multocida. Amplification of an 846-nucleotide fragment of toxA gene was developed. The fragment amplified by PCR was detected in P multocida type D not type A. The PCR amplification was as sensitive as it could detect 1 pg of P multocida DNA. We compared the result of the PCR with the enzyme linked immunosorbent assay (ELISA) in a test for 40 swine nasal swabs. All of these isolates were toxin negative based on the ELISA while 2 isolates were detected in the PCR technique. in addition to accuracy, as required for rapid detection from contaminated nasal swabs, toxigenic P multocida was recovered efficiently from contaminated culture without inhibition of the PCR. The results show that the PCR detection of toxigenic P multocida directly form nasal swabs are feasible.

돼지 위축성 비염백신의 효과에 관한 연구

지영철,로 승,한정희,한태욱,Chi, Yongzhe,Lu, Cheng,Han, Jeong-hee,Hahn, Tae-wook 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.4

Atropic rhinitis (AR) is one of major respiratory diseases in pigs. AR causes a great economic losses and is considered to be a multifactorial disease in which herd management, heredity, and environment. Several vaccines against have been developed commercially and used in pig farms but the efficacy of each vaccine is still questionable. In this study, one of commercial AR vaccines, which contains inactivated Bordetella bronchiseptica, Pasteurella multocida type D and their toxoid was evaluated for vaccine efficacy by challenge test. Twenty piglets were divided into four groups as follows; group I was piglets from vaccinated sows (twice before parturition); group II was piglets from vaccinated sows (same as group I) and were vaccinated at 1 day old; group III and IV were piglets without any vaccination. Groups I, II, and III were challenged by intranasal instillation of $5.3{\times}10^7$ CFU of B bronchiseptica twice and $1{\times}10^9$ CFU of P multocida five times. Group IV was control group without any vaccination and any challenge. We compared serological results, recovery rate of P multocida by polymerase chain reaction, clinical signs and pathological findings between vaccinated groups and unvaccinated groups for efficacy of the vaccine, Serological responses against B bronchiseptica and toxigenic P multocida type D were not showed evident discrepancy between vaccinated groups and unvaccinated groups assuming that the antibody responses against the vaccine is very delayed. However, growth rate, clinical signs and snout lesion grading in vaccinated groups showed more favorable than those in unvaccinated group. Therefore, AR vaccination in this study is considered to be effective in the prevention of AR in pigs.

여러가지 산촉매하에서 CFC -113 의 산화분해

지영철,장원철,이태진 ( Young Chul Ji,Won Chul Chang,Tae Jin Lee ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.1

돼지 유행성 설사 바이러스 국내분리주의 유전학적 특성 규명

지영철,권혁무,정현규,한정희,Chi, Yong-zhe,Kwon, Hyuk-moo,Jeong, Hyun-kyu,Han, Jeong-hee 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

Porcine epidemic diarrhea virus(PED), a member of Coronaviridea, is the etiological agent of enteropathogenic diarrhea in swine. The purpose of this study was to investigate genetic characteristic of PEDV isolated in Korea. Nucleocapsid(N) gene and membrane (M) gene of recent Korean PEDV strains isolated in 2001 were amplified, cloned, sequenced and analyzed. N gene of seven Korean PEDV field isolates bad 94.5% to 99.4% nucleotide and 92.4% to 99.4% amino acid sequence homology each other. Nucleotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 95.1% to 98.0% nucleotide and 93.5% to 97.6% amino acid sequence homology. By phylogenetic tree analysis on based nucleotide sequences, PEDVs were clustered into four groups. By phylogenetic tree analysis based on amino acid sequences. PEDVs were clustered into five groups. M gene of our Korean PEDV field isolates had 99.6% to 100% nucleotide and 98.7% to 100% amino acid sequence homology each other. Nuclotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 98.5% to 98.8% nucleotide and 97.3% to 97.8% amino acid sequence homology. By phylogenetic tree analysis based on nucleotide and amino acid sequences, PEDVs were clustered into two groups which were Korean PEDV isolate group and foreign PEDV isolate group.

항혈청 투여에 의한 돼지 전염성 위장염의 예방효과 II.임상검사, 병리조직학적 검사, 면역조직화학적 검사

지영철,한정희,권혁무,정현규,Chi, Yong-Zhe,Han, Jeong-Hee,Kwon, Hyuk-Moo,Jeong, Hyun-Kyu 한국수의병리학회 2003 한국수의병리학회지 Vol.7 No.1

The purpose of this study was to investigate protective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 2hrs, 24hrs and 36hrs after birth. Five piglets administered with the TGEV antiserum were experimentally challenged with TGEV at four-day-old. Control group was four piglets challenged with TGEV only. Clinical signs and gross, histopathological and immunohistochemical findings were examined. In clinical signs, piglets of the control group appeared the typical signs such as severe watery diarrhea, depression and anorexia but piglets of the TGEV antiserum adminstered group recovered progressively. In clinical signs, piglets of the control group appeared the typical signs such as severe watery diarrhea, depression and anorexia but piglets of the TGEV antiserum adminstered group recovered progressively. In mortality, control group showed 75%, but TGEV antiserum adminstered group showed 20.0 %, respectively. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach. But gross findings of piglets of the TGEV antiserum adminstered group appeared milder than them of control group. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But histopathological findings of piglets of the TGEV antiserum adminstered group appeared milder than them of control group. In immunohistochemical findings, piglets of the TGEV antiserum adminstered group showed more intensive in reaction for IgA and IgG than them of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of TGEV antiserum to piglets was effective to prevent TGEV infection and reduce their mortality.

여러 가지 산촉매상에서 CFC - 113 의 산화분해

지영철,장원철,이태진 ( Young Chul Ji,Won Chul Chang,Tae Jin Lee ) 한국화학공학회 1996 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.34 No.4

The catalytic oxidative decomposition of CFC-113 was carried out on various metal oxides at atmospheric pressure, 500℃, and CFC-113 feed mole percent of 0.5 using a tubular flow rector. Acid catalysts such as aluminas, zeolites, and titania-silica exhibited high decomposition activities, whereas the activities of Fe₂O₃, ZnO, TiO₂, SiO₂, and CaO were low. TiO₂-SiO₂ catalyst showed the best performance: regarding retention of activity and high selectivity to CO₂ among the acid catalysts investigated. However, it was found that deactivation of catalyst occurred because Si in TiO₂-SiO₂(Ti/Si=50/50) reacted with inorganic halogens(fluorine and chlorine) which were reaction products. Solid superacid catalyst(TiO₂-SiO₂/SO₄^(2-):(Ti/Si=50/50) modified with H₂SO₄ was prepared for high durability against inorganic halogens. The acid sites distribution of the catalyst was measured by DSC. The result suggested that strong acid sites were active sites. This superacid catalyst showed the highest activity and durability among the catalysts examined.

항혈청 투여에 의한 돼지 전염성 위장염의 예방효과 2. 임상검사, 병리조직학적 검사, 면역조직화학적 검사

지영철 ( Yong Zhe Chi ),한정희 ( Jeong Hee Han ),권혁무 ( Hyuk Moo Kwon ),정현규 ( Hyun Kyu Jeong ) 한국수의병리학회 2003 한국수의병리학회지 Vol.7 No.1

항혈청 투여에 따른 돼지 유행성 설사병 예방효과 2. 임상증상, 병리조직학적 검사, 면역조직학적 검사

지영철 ( Yong Zhe Chi ),한정희 ( Jeong Hee Han ),권혁무 ( Hyuk Moo Kwon ),정현규 ( Hyun Kyu Jeong ),이함희 ( Ham Hee Lee ) 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (PEDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Clinical signs and gross, histopathological lesion and immunohistochemical findings were examined. The results obtained were as follows; 1. In clinical signs, piglets of the control group appeared the typical signs of severe watery diarrhea, depression and anorexia but piglets of the PEDV antiserum treated group recovered progressively. In mortality, control group showd 75%, but PEDV antiserum treated group showed 16.7%, respectively. 2. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 3. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 4. In immunohistochemical findings, piglets of the PEDV antiserum treated group showed more intensive in reaction for IgG and IgG than those of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of PEDV antiserum to piglets was effective in preventing PEDV infection and reduced their mortality.

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 1. 혈청학적 결과, RT-PCR 검사, 형광항체검사

지영철 ( Yong Zhe Chi ),한정희 ( Jeong Hee Han ),권혁무 ( Hyuk Moo Kwon ),한태욱 ( Tae Wook Hahn ),정현규 ( Hyun Kyu Jeong ),박봉균 ( Bong Kyun Park ) 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.

항혈청 투여에 따른 돼지 전염성 위장염 예방효과 1. 혈청학적 결과, RT-PCR 검사, 형광항체검사

지영철 ( Yong Zhe Chi ),한정희 ( Jeong Hee Han ),권혁무 ( Hyuk Moo Kwon ),한태욱 ( Tae Wook Hahn ),정현규 ( Hyun Kyu Jeong ),박봉균 ( Bong Kyun Park ) 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24 hrs and 36 hrs after birth. five piglets adiminstered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization (SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT-PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3. The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.