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사람 적혈구에 있어서 극성 대사물질과 막 구성분의 Uptake에 관한 1-Palmitoyllysolecithin과 인삼 Saponin의 영향
정진성,이종호,조기승,주충노,Chung, Jin-Sung,Lee, Jong-Ho,Cho, Key-Seung,Joo, Chung-No 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
사람 적혈구에서 [$^{14}C$]-alanine, [$^{14}C$]-glucose, [$^{14}C$]-cholesterol 및 [$^{14}C$]-phosphatidylethanolamine의 흡수를 lysolecithin과 saponin 존재하에서 실험하여 본 결과 다음과 같은 결과를 얻었다. 적혈구를 lysolecithin으로 미리 처리시켰을 때, 상기 물질들의 uptake가 alanine의 경우 최고 111%, glucose는 43%, cholesterol은 48%, phosphatidylethanolamine은 17%의 상승율을 보였고, saponin의 처리에 의해서는 각각 408%, 70%, 28% 및 10%의 상승율을 나타냈다. 두 lytic agent에 의한 상승율은 각각의 최적 농도에서 비교해보면 lysolecithin은 saponin 경우보다 막성분인 cholesterol이나 phosphatidylethanolamine의 uptake에 더 영향을 끼쳤고, 한편 saponin은 alanine과 glucose와 같은 polar metabolite의 uptake에 더 영향을 주는 것으로 확인되었다. The effects of lysolecithin and ginseng saponin on the uptake of [$^{14}C$]-alanine, [$^{14}C$]-glucose, [$^{14}C$]-cholesterol, and [$^{14}C$]-phosphatidylethanolamine to human erythrocytes in the various conditions were as follows. When the human erythrocytes pretreated in the presence of low lytic amount of lysolecithin, the uptake of such polar metabolites and membrane components was enhanced up to [$^{14}C$]-alanine 111%, [$^{14}C$]-glucose 43%, [$^{14}C$]-cholesterol 48%, and [$^{14}C$]-phosphatidylethanolamine 17%, In case of saponin pretreatment, the uptakes of such substances were increased to 408%, 70%, 28%, and 10%, respectively. In comparison with both lytic surfactants under the optimum concentration, lysolecithin was more effective to the uptake of membrane components, cholesterol and phosphatidylethanolamine. On the other hand, saponin was more potent to the polar metabolite uptake into the erythrocytes.
개불(Urechis unicinctus) 저정낭내(貯精囊內) 웅성생식세포(雄性生殖細胞)의 분화(分化)
정진욱,최임순,신길상,주충노,Jeong, Jin-Wook,Choe, Rim-Soon,Shin, Kil-Sang,Joo, Chung-No 한국현미경학회 1989 Applied microscopy Vol.19 No.1
The storage sac of U. unicinctus can be divided into two parts morphologically as well as functionally ; one is proximal and the other distal storage sac. It is because of the seasonal morphological change of the storage sac caused by sperm accumulation in the storage sac. The proximal storage sac contains the mature sperm with the dumbbell-shaped acrosome and well developed one or two mitochondria in the middle piece, whilst the sperm of hemispherical acrosome associate closely with an accessory cell in the distal storage sac. This means that the sperm do not perform the synchronous development in a storage sac, which is not the case of sperm development in the coelomic cytophorus. In addition, the basal membrane of the distal storage sac is different from that of the proximal storage sac in term of tissue formation. Connective tissues, acces-sory cells and small lumen develop on the basal membrane of the distal sto-rage sac, which is well contrasted with the thin basal membrane of the proximal storage sac. The function of the storage sac is discussed in rela-tion of the sperm development and the seasonal change.
사람 적혈구의 영양물 흡수에 대한 Palmitoylcrnitine 과 Palmitoylcholine 의 영향
정진성,이종호,조기승,주충노 ( Jin Sung Chung,Jong Ho Lee,Key Seoung Cho,Chung No Joo ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4
The effects of polmitoylcarnitine and palmitoylcholine on the uptake of ^(14)C-alanine, ^(14)C-glucose, ^(14)C-cholesterol and ^(14)C-phosphatidylethanolamine to human erythrocytes in the various conditions were investigated.. When the human erythrocytes were pretreated in the presence of 0.05 μmole, 0.02 fr mole, and 0.05 μmole of palmitoylcarnitine and palmitoylcholine, uptake of ^(14)C-alanine was enhanced from 87% to 156%, ^(14)C-glucose from 10% to 45%, ^(14)C-cholesterol from 2.5% to 44%, and ^(14)C-phosphatidylethanolamine from 8% to 16% as compared with control group.
사람 적혈구에 있어서 극성 대사물질과 막 구성분의 Uptake 에 관한 1 - Palmitoyllysolecithin 과 인산 Saponin 의 영향
정진성,이종호,조기승,주충노 생화학분자생물학회 1986 BMB Reports Vol.19 No.2
The effects of Lysolecithin and ginseng saponin on the uptake of [^(14)C]-alanine, [^(14)C]-glucose, [^(14)C]-cholesterol, and [^(14)C]-phosphatidylethanolamine to human erythrocytes in the various conditions were as follows. When the human erythrocytes pretreated in the presence of low lytic amount of lysolecithin, the uptake of such polar metabolites and membrane components was enhanced up to [^(14)C]-alanine 111%, [^(14)C]-glucose 43%, [^(14)C]-cholesterol 48%, and [^(14)C]-phosphatidylethanolamine 17%, In case of saponin pretreatment, the uptakes of such substances were increased to 408%, 70%, 28%, and 10%, respectively. In comparison with both lytic surfactants under the optimum concentration, lysolecithin was more effective to the uptake of membrane components, cholesterol and phosphatidylethanolamine. On the other hand, saponin was more potent to the polar metabolite uptake into the erythrocytes.