인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영,Jo, Joon-Seung,Chung, Tai-Ho,Kwak, Chun-Sik,Kim, Choong-Young 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1

인삼 성분을 메탄올로써 추출하고 이것이 쥐에 있어서 당질의 산화와 당의 생성 즉 glycogenesis 및 glyconeogenesis 그리고 당 신생반응의 첫 단계 반응을 조절하는 효소라고 믿고 있는 간의 phosphoenolpyruvate carboxylkinase의 활성에 어떠한 영향을 미칠것인가를 조사하여 보았다. 인삼 추출물을 쥐 체중 100g당 5mg이 되도록 생리적 식염수 0.5ml에 녹여서 1일 1회 4일간 복강내로 미리 투여하였으며 대조군에는 같은 양의 식염수만 주입하였다. 이것을 다시 계속 섭식시킨 군과 24시간 굶긴 군으로 나누어서 인삼 추출물을 투여한 1시간뒤에 glucose-$^{14}C$ 또는 amino acid mixed-$^{14}C$를 주입하여 호기중애 배출되는 $^{14}CO_2$ 양을 검출하고 또 3시간 후에 간속의 glycogen과 phospho-enolpyruvate caboxykinase에 대해서 검토하였다. 이 실험 결과 인삼은 glucose의 산화를 현저히 증가시켰으며 이 효과는 기아상태에서 더욱 촉진적으로 작용하였다. 그러나 인삼은 간에서의 glycogenesis나 glyconeogenesis에 대해서는 효능이 그리 없는것 같았다. 즉 인삼투여군은 대조군에 비하여 간의 glycogen농도나 $^{14}C$ 화합물의 glycogen에의 편입속도가 다같이 별 차이를 나타내지 않았다. 그리고 간의 phosphoenolpyruvate carboxykinase의 활성에도 인삼의 별 영향을 미치지 않았다. Cultivated dry ginseng root was pulverized and extracted with methanol. The extractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200~220g were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5mg per 100g body weight of the ginseng extract in 0.5ml of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 uc of glucose-$^{14}C$ with carrier 100mg glucose per 100g of body weight or $4\;{\mu}c$ of amino acid mixed-$^{14}C$ with carrier 20mg L-alanine per 100g of body weight were injected peritoneally for the correspond group and control. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase, To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately after the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-$^{14}C$ or amino acid mixed-$^{14}C$ in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the adminisration of ginseng both the time of feeding and fasting.

인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영 ( Joon Seung Jo,Tai Ho Chung,Chun Sik Kwak,Choong Young Kim ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

Cultivated dry ginseng root was pulverized and extracted with methanol. Theextractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200∼2208 were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5㎎ per 1008 body weight of the ginseng extract in 0.5㎖ of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 μc of glucose-^(14)C with carrier 100㎎ glucose per 1008 of body weight or 4 μc of amino acid mixed-^(14)C with carrier 20㎎ L-alanine per 1008 of body weight were injected peritoneally for the correspond group and control.. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase. To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately alter the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-^(14)C or amino acid mixed-^(14)C in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the administration of ginseng both the time of feeding and fasting.

흰쥐재생간의 Alkaline Phosphatase의 활성치

곽춘식,조준승,Kwak, Chun-Sik,Jo, Joon-Seung 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.2

간을 부분 절제했을 때의 재생산 및 혈청의 alkaline photiphatase (ALP)의 변동을 관찰하기 위하여 건강한 흰쥐를 사용하여 간을 약 70% 부분 절제하고 10일 동안 경시적으로 재생간 및 혈청중의 ALP의 총 활성과 그 isoenzyme 분획을 측정하고 아울러 혈청 총 bilirubin과 GTP, 그리고 재생간에서의 간 단백 및 혈청 단백의 합성 속도 등을 측정하여 상호 비교 검토하였다. 재생간의 ALP치는 간엽 부분 절제후 그 활성이 증가하여 수술후 2일에 최고치에 달하고 이후 감소되어 정상으로 회복되었다. 또한 혈청 ALP의 변동도 재생간의 그것과 유사한 경향을 보여 수술후 12시간부터 급격히 증가하여 2일에 최고치에 달하고 이후 감소되었으나, 10일째에도 정상으로 회복되지는 않았다. 그리고 쥐 혈청의 ALP는 간성분획 및 장성 분획의 두 Bozyme으로 구분되며, 장성 분획이 간성 분획보다 약 2배 많았으며, 이두 isozyme은 간엽 절제후에 다같은 경향으로 변동하였다. 혈청 GTP의 활성도 간엽 절제후 곧 상승하여 12시간에 최고치에 달하고 이어 감소하여 6일째에 정상으로 회복되었다. 그러나 혈청 총 bilirubin의 함량은 별 변동은 보이지 않았다. 재생간에서 amimo acid mixture-$^{14}C$의 간 단백내의 편입 속도는 상승을 보여 수술후 3일 까지 약 2배로 높은 치를 유지하다가 10일째에는 정상치로 회복되었다. 그러나 이 amimo산의 혈청 단백내의 편입 속도는 계속 낮은 치를 보여 수술 후 2일째에 0.6 배로 가장 낮았으며 10일이 지나도 완전 회복되지는 않았다. 이상의 성적으로 보아 간엽 절제후 간 재생 시기에는 간 및 혈청의 ALP의 활성 증가가 일어나며, 이것은 간에서 효소 합성의 항진에 의할 것으로, 생각되며 이때 담즙 울체현상은 없으므로 bilirubin은 이 효소의 활성 증가와 무관한 것으로 생각된다. The studies on changes of hepatic and serum alkaline phosphatase activities in 70% hepatectomized rats were performed and also compared with the level of isozymes of serum alkaline phosphatase, serum total bilirubin, serum glutamic pyruvic transaminase and incorporation rates of amino acid mixture-$^{14}C$ into hepatic and serum proteins in the same rats. After partial hepatectomy of the rats, hepatic alkaline phosphatase activities were significantly increased in the period of 0.5 to 6 days with its peak level on the second day and returned to normal after ten days. The alkaline phosphatase activities significantly increased in the serum of the same rats with its peak level on the second day and increased activities has sustained during the ten days. In non-hepatectomized rats intestinal isozyme of alkaline phosphatase was predominantly increased than the liver isozyme with 2 folds increase and the similar isozyme pattern was observed with absolutely increased level in hepatectomized rats. The activity of serum glutamic pyruvic transaminase also elevated from 12 hours to two days after partial hepatectomy in rats. No significant changes in serum total bilirubin contents were noted throughout the experiments. The incorporation rate of amino acid mixture-$^{14}C$ into hepatic protein showed an immediate increase after partial hepatectomy and reached maximum after 24 hours and these levels continued to be high throughout the six days. The incorporation rate into serum total protein, on the other hand, significantly decreased in the regenerating stage. The most decreased level of total protein was seen on the second day.

간엽 부분 절제한 흰쥐에 있어서 혈청 Ceruloplasmin 의 함량 변동

곽춘식,조준승 ( Chun Sik Kwak,Joon Seung Jo ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.4

This study concerns an observation noted in changes of. serum ceruloplasmin after hepatectomy (removal of 70% of the liver) in rats. These data were compared with those of several values of liver function tests and incorporation rates of amino acid mixed-^(14)C into serum protein in the same rats. After partial hepatectomy of the rats, serum ceruloplasmin contents were significantly increased in the span of two to three days (peak on the third day). However, the values returned to normal after six days. The levels of alkaline phosphatase in the serum of same rats significantly increased after hepatectomy to reach its peak on the second day and these levels continued to be high throughout the 10 days. The activity of serum glutamic pyruvic transaminase was also elevated 12 hours to two days following partial hepatectomy in rats. No significant changes in total bilirubin contents were noted throughout the experiments. Serum total protein contents and incorporation of amino acid mixed-^(14)C into serum protein were significantly decreased in the regenerating stage. The most decreased values were seen after two days in incorporation rate and three days in serum total protein contents.

흰쥐 재생간의 Alkaline Phosphatase 의 활성치

곽춘식,조준승 ( Chun Sik Kwak,Joon Seung Jo ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.2

The studies on changes of hepatic and serum alkaline phosphatase activities in 70% hepatectomized rats were performed and also compared with the level of isozymes of serum alkaline phosphatase, serum total bilirubin, serum glutamic pyruvic transaminase and incorporation rates of amino acid mixture-^(14)C into hepatic and serum proteins in the same rats. After partial hepatectomy of the rats, hepatic alkaline phosphatase activities were significantly increased in the period of 0.5 to 6 days with its peak level on the second day and returned to normal after ten days. The alkaline phosphatase activities significantly increased in the serum of the same rats with its peak level on the second day and increased activities has sustained during the ten days. In non-hepatectomized rats intestinal isozyme of alkaline phosphatase was predominantly increased than the liver isozyme with 2 folds increase and the similar isozyme pattern was observed with absolutely increased level in hepatectomized rats. The activity of serum glutamic pyruvic transaminase also elevated from 12 hours to two days after partial hepatectomy in rats. No significant changes in serum total bilirubin contents were noted throughout the experiments. The incorporation rate of amino acid mixture-^(14)C into hepatic protein showed an immediate increase after partial hepatectomy and reached maximum after 24 hours and these levels continued to be high throughout the six days. The incorporation rate into serum total protein, on the other hand, significantly decreased in the regenerating stage. The most decreased level of total protein was seen on the second day.

Ethanol 중독 흰쥐에서 총담관결찰이 간의 Glutathione S-Transferase, Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,김여희,조준승,Kwak, Chun-Sik,Kim, You-Hee,Jo, Joon-Seung 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

급성 및 만성 주정중독 흰쥐에서 담즙울체가 간의 glutathione S-transferase(GST), glutathione reductase(GR) 및 glutathione peroxidase(GSH-Px) 활성 변동에 어떤 영향을 미치는가를 알아보기 위하여 이 실험을 하였다. 흰쥐에게 만성 주정중독을 시켰을 때 간의 세포질과 microsome 분획의 GST 활성도는 약간 증가 되었다. 그러나 mitochondria 분획의 GST 활성도는 변동을 나타내지 않았다. 또한 만성 주정 중독군에서 간의 GR 및 간의 세포질 분획의 GSH-Px의 활성도와 급성 주정중독군에서 간 세포질, mitochondria 및 microsome 분획의 GST, 간의 GR과 간의 세포질 분획의 GSH-Px 활성도 등도 변동이 없었다. 만성 주정중독 후 총담관을 결찰한 군에서 간 세포질 분획의 GST 활성도는 실험의 전기간을 통해 의의 있는 활성 감소를 나타내었다. 그러나 감소의 정도는 총담관결찰군보다 현저하지 않았다. 그리고 간세포 mitochondria 분획에서 GST 활성도는 총담관결찰군이 총담관결찰 후 14일에 약간 감소되었으나 만성 주정중독 후 총담관을 결찰한 군에서는 2일 부터 14일까지 의의 있는 감소를 나타내었다. 또한 microsome 분획의 GST 활성도는 총담관결찰군에서는 총담관결찰 후 7일 및 14일에 현저한 증가를 나타내었다. 그러나 만성 주정증독 후 총담관을 결찰한 군에서는 실험의 전기간을 통해 증가를 보이지 않았다. 만성 주정중독 후 총담관을 결찰한 군에서 간의 GR 활성도는 총담관결찰 후 2일부터 14일까지 증가를 나타내었으나 그 증가의 정도는 총담관결찰군보다 현저하지 않았다. 반면에, 만성 주정중독 후 총담관을 결찰한 군에서 GSH-Px 활성도는 총담관결찰 후 14일에 의의 있는 감소를 나타내었다. 그러나 그 감소의 정도는 총담관결찰군보다 현저하지 않았다. 총담관결찰 14일 후 급성 주정증독을 시킨 군에서 간 세포질 분획의 GST와 GSH-Px의 활성도는 현저한 감소를 나타내었다. 그러나 그 활성 감소의 정도는 총담관결찰만 하고 14일에 희생시킨 군보다는 현저하지 않았다. 총담관결찰 14일 후 급성 주정중독을 시킨 군에서 간세포 microsome 분획의 GST와 간의 GR 활성도는 현저한 증가를 나타내었다. 그러나 그 활성 증가의 정도는 총담관결찰만 한 후 14일에 희생시킨 군보다 현저하지 않았다. 총담관결찰 14일 후 급성 주정중독을 시킨 군에서 간세포 mitochondria 분획의 GST 활성도는 총담관결찰만 한 후 14일에 희생시킨 군과 마찬가지로 의의 있는 활성 감소를 나타내었다. 이상 실험결과를 보아 급성 및 만성 주정중독시 간에 담즙울체가 야기되면 endoplasmic retieulum의 GST와 세포질의 GR 활성도가 간에 담즙울체만 야기시켰을때 보다 그 활성이 감소되는 것으로 보이며 반면에 간의 세포질 분획의 GST와 GSH-Px 활성도는 증가되는 것 같았다. 그리고 만성 주정중독시에만 세포질과 endoplasmic reticulum의 GST 활성도가 약간 증가되는 것 같았다. This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase(GST), glutathione peroxidase(GSH-Px) and glutathione reductase(GR) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5%(v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver. The liver GR activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for measuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chronically ethanol intoxicated group but the mitochondrial GST activities did not increase in this group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significant changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or acutely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxication showed of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxication showed significant decrease at the 2nd, 3rd, 7th and 14th days following the operation. The liver microsomal GST activities of the (CBD) ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerable increase at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activities. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkable decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligation. The liver microsomal GST liver GR activities, however, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligation. At the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligation.

Ethanol 중독 흰쥐에서 총담관결찰이 간의 Glutathione S - Transferase , Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,김여희,조준승 ( Chun Sik Kwak,You Hee Kim,Joon Seung Jo ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase(GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5% (v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver. The liver GR activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for measuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chronically ethanol intoxicated group but the mitochondrial GST activities did not increase in this group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significant changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or acutely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligation after chronic ethanol intoxication showed significant decrease at the 2nd, 3rd, 7th and 14th days following the operation. The liver microsomal GST activities of the CBD ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerable increase at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activities. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less degree on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkably decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligation. The liver microsomal GST and liver GR activities, however, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligation. At the 1.5th and 24th following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligation.

血淸 Alanine Aminotransferase와 Alkaline Phosphatase의 活性에 대한 饑餓 및 人蔘의 效果

邊永宙,郭春植,曺準承 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.1

絶食시켰을 때 肝機能檢査時에 많이 사용되는 血淸 alanine aminotransferase와 alkaline phosphatase거 어떻게 變化하며 또 人蔘의 投與가 이것에 어떠한 效果를 미치는가를 알아 보기 위하여 體重이 約 250g되는 Sprague-Dawley種의 흰쥐를 使用하여 調査해 본 結果는 다음과 같았다. 動物을 絶食시키므로써 體重은 1日에 約 10%씩 減少되었으며, 人蔘을 投與해도 絶食으로 因한 體重減少에는 別다른 영향을 미치지 않았다. 血淸 alanine aminotransferase의 活性은 絶食後 減少되어 絶食 3日째에는 約 5分의 2로 顯著히 낮은 値를 보였다. 人蔘의 投與는 絶食으로 因한 이 酵素活性의 低下를 뚜렷하게 沮止하는 效果를 나타내었다. 한편 血淸 alkaline phosphatase는 絶食시켜도 別다른 變動을 나타내지 않았으며 따라서 人蔘은 이 酵素의 活性에는 영향을 미치질 않았다. Changes in serum alanine aminotransferase and alkaline phosphatase activities were studied in Sprague-Dawley rats by starvation and ginseng. In starved rats, body weight were decreased in rate by 10% a day and weight decrease was not affected by ginseng administration. Serum alanine aminotransferase activities were decreased by starvation and revealed 60% decrease on the third day of starvation. Ginseng administration significantly inhibited this decrease of enzyme activity produced by starvation, while serum alkaline phosphatase revealed no noticeable changes by starvation, and ginseng administration.

食餌性 指肪의 含量과 種類가 흰쥐 Lipoprotein의 分劃像에 미치는 影響

李宇炯,郭春植,曺準承 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.1

食餌脂肪의 含量 및 그 種類가 血淸 lipoprotein 分劃에 어떠한 影響을 미치는가를 調査하기 위하여 離乳된 쥐를 10마리씩 3群으로 나누어서 12% calorie 脂肪食餌를 對照로 하여 植物性高脂肪食餌로서 45% calorie corn oil 食餌와 動物性高脂肪食餌로서 45% calorie butter 食餌를 各各 12週間 投與한 後에 各群의 血淸 lipoprotein 分劃을 測定하여 다음과 같은 結果를 얻었다. 對照인 正常食餌群의 血淸 chylomicron, VLDL, LDL 및 HDL 値는 各各 36.4±10.7㎎/㎗, 15.9±5.2㎎/㎗, 26.2±4.7㎎/㎗ 및 93.3±18,7㎎/㎗이었다. 對照群과 比較해서 45% corn oil 投與群의 血淸 lipoprotein 分劃値는 別로 差異를 나타내지 않았으나 45% butter 投與群에서는 chylomicron, VLDL 및 LDL 値는 모두 1.5倍 以上 높았으며 反對로 HDL 値는 13%가량 떨어지는 傾向을 나타내었다. 以上의 結果로 보아 대부분의 不飽和脂肪酸으로 構成된 脂肪食餌를 投與했을 때는 LDL 以外는 別로 變化가 일어나지 않으나 飽和脂肪酸이 많은 動物性脂肪을 多量攝取하면 血淸 lipoprotein의 모든 分劃像에 變化를 招來한다고 할 수 있다. Effect of a high level and types of dietary fat on serum lipoproteins was investigated in Sprague-Dawley rats weighing 60-65gm. The animals were divided into three groups and were fed 3 different fat diet: 12% calorie fat diet, 45% calorie-butter fat diet, and 45% calorie-corn oil diet. The diets were isoenergetic (4.4Kcal/gm), and were fed to the rats ad libitum for 12 weeks. Mean serum lipoprotein levels in mg/dl for the control group were 36.4±10.7: chylomicron, 15.9±5.2: VLDL, 26.2±4.7: LDL, and 93.3±18.7: HDL. The high corn oil diet did not significantly change the levels of serum lipoproteins except 1.5-fold increase in LDL. However, the high-butter fat diet showed 1.5-to 2-fold increase in serum levels of chylomicron. VLDL and LDL, but no change in HDL level. These result indicate that long-term feeding fo high-fat diet composed of primarily saturated fatty acid increases serum levels of lower dinsity fractions of lipoprotein.

실험적 간흡충증에 있어서 혈청 Ceruloplasmin 활성도에 관하여

곽춘식,박성대,조준승 慶北大學校 醫科大學 1971 慶北醫大誌 Vol.12 No.2

A blue-green copper binding protein was first isolated and called ceruloplasmin in 1948 by Holmberg and Laurell. They also demonstrated that ceruloplasmin has a molecular weight of about 151,000 and contains 0.34% copper, or about 8 atoms of copper per mol. Normal plasma contains about 30 ㎎ of ceruloplasmin per 100 ㎖, and about 96% plasma copper combines with this protein founded in alpha_2-globulin fraction. Ceruloplasmin shows oxidase activity in vitro toward several amine, including epinephrine, serotonine, and dihydroxyphenylalanine. However, Osaki and coworkers have found that ceruloplasmin exerts a catalytic activity in plasma to convert Fe^++ to Fe^+++ and thus promote the rate of incorporation of iron into transferrin, and in 1966 suggest that it be redesignated serum ferroxidase. According to many reports, ceruloplasmin levels, determined as oxidase activity, are elevated in several circumstances, such as pregnancy, schizophrenia, bacterial infection, hepatitis, cirrhosis, etc, and lowered in Wilson's disease, nephrotic syndrome and protein deficient malnutrition. However, it reports little concerning the change of ceruloplasmin activity during chronic damage of the liver by the infection of clonorchis sinensis. This paper presents the results of the variation of ceruloplasmin activity following the infestation of Clonorchis sinensis in rabbits, and the correlation between its activity and the values of several liver function tests of alpha_2-globulin in the same rabbit's sera. Fifteen rabbits, with an average body weight of 2 ㎏, were infested through the mouth with 600 metacercaria of Clonorchis sinensis, collected from the muscle of the silver carp, Pseudorasbora parva, from the pond in the vicinity of Taegu. For 8 weeks after the infestation, weekly variation of the levels of serum ceruloplasmin activity by method of Henry, Chiamori and Segalove, bilirubin, cholesterol, alkalne phosphatase activity, thymol tburidity test, SGOT, SGPT, alpha_2-globulin estimated by total protein and percentage of electrophoretic distribution, and body weight were observed. The following results were obtained: 1. Serum ceruloplasmin activity curve shows a continuous rise from 2nd week after clonorchial infestation, especially remarkable in the initial stage. 2. Serum bilirubin, cholesterol and alkaline phosphatase activity curves, almost same pattern, also show a continuous rise from 3rd or 4th week after the infestation, but early stage curve is gradual. Serum tranaminase activity curves show initial rise, 2nd week, followed by a decline. Thymol turbidity test does not respond to the clonorchiasis. 3. Serum alpha_2-globulin level drops sharply at 2nd week after the intestation followed by a rise, and does not show a significant change from 4th week after the infestation. 4. In comparison with the curves for serum ceruloplasmin activity, a few liver function tests and alpha_2-globulin, the curve of ceruloplasmin is similar to those of bilirubin, cholesterol and alkaline phosphatase, even though the former shows a more sensitivie rise than the laters in the initial stage, whereas the curve of ceruloplasmin is not related to those of alpha_2-globulin level. 5. Body weights of metacercaria infested rabbits were decreased continuously until 3rd or 4th week followed by retern to the initial weight by 7th or 8th week after the infestation. Stool test for clonorchial egg was positive at the end of 3rd week after the infestation. After 8 weeks of metacercarial administration, gross finding of the liver showed a enlarged, nodular and considerable cirrhotic change.