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芸香科 ( 운향과 ) 식물 종실의 항산화성 물질에 관한 연구
김성진,김지수,조영계 ( Seong Jin Kim,Ji Soo Kim,Yong Goe Joh ) 한국유화학회 1994 한국응용과학기술학회지 Vol.11 No.1
Some seeds of the Rutaceae family, Zanthoxylum piperitum, Z. schinifolium, Evodio officinalis. Poncirus trifoliata, Citrus unshiu, were investigated to clarify their antioxidative components. Finely powdered samples were extracted by hexane, followed by dichloromethane and then 70% methanol in a hot bath. Its unsaponifiables containing α-and y-tocopherol with trace amount of β-and δ-tocopherol also showed comparatively weak activity. Levels of total tocopherols in the samples averages 42. 24-154. 11 mg/100g total extractives. The dichloromethane-and 70% methanol extractives showed strong antioxidative activity, from which antioxidative substances were purified with benzene-acetone(6 : 5. V/V) on a silica gel column, and with a solvent mixture of acetonitrile-methanol-H_2O(40 : 40 : 20, V/V/V) on a Sep-Pak C_18 hydrolyzed by 5% KOH-ethanol. The recovered unsaponifiables were then, separated on a column of high performance liquid chromatography. The unsaponifiables produced by hydrolysis of the isolates from dichloromethane extractives has epi-catechin(40.0~57.1%) and (+)-catechin(19.1~24.4% to total phenolic substances, on area base) as major component, accompanied by chlorogenic acid, gallic acid(?), trans-p-coumaric acid and trans-p-ferulic acid including some unknown components, and those derived from 70% methanol extractives also comprise (+)-catechin(31.3~39.6%) to total components. on area base), epi-catechin(20.2~36.4%), trans-p-coumaric acid(8.4~15.3%) and trans-p-ferulic acid(7.7~14.1%) as predominant component with some minor coponents, but the fraction supposed to be gallic acid(?) is not present. The antioxidative activities of the phenolic components isolated in this work were in order of epi-catechin>catechin>chlorogenic acid>trans-p-ferluic acid>trans-p-coumaric acid.
ScienceON
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