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PDMS와 고분자 전해질 표면을 이용한 간편한 세포 패터닝 방법
정헌호(Heon-Ho Jeong),송환문(Hwan-Moon Song),황예진(Ye-jin Hwang),황택성(Taek-Sung Hwang),이창수(Chang-Soo Lee) 한국생물공학회 2009 KSBB Journal Vol.24 No.6
This study presented facile method of cell patterning using fabricated PDMS patterns on polyelectrolyte coated surface. This basic principle is the fabrication of functional surface presenting two orthogonal surfaces such as cell adhesive and repellent properties. Cell adhesive surface was firstly fabricated with simple coating of polyelectrolyte multilayer. And then, the desired patterns of PDMS for the prevention of nonspecific binding of cells were transferred onto the previously formed thin film of polyelectrolyte multilayer. Thus, we could prepare novel functional surface simultaneously containing PDMS and polyelectrolyte region. As expected, the PDMS regions showed effective prevention of nonspecific binding of cell and the other region, exposed polyelectrolyte area, provided cell adhesive environment. The height of formed PDMS structure was about 100 nm. Based on this method, cell patterning can be successfully obtained with various pattern shapes and sizes. Therefore, we expect that this simple method will be useful platform technology for the development of cell chip, cell based assay system, and biochip.
유동-집속 생성기의 병렬화를 통한 에멀젼 생산속도 향상
정헌호 ( Heon-ho Jeong ) 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.5
Droplet-based microfluidic device has led to transformational new approaches in various applications including materials synthesis and high-throughput screening. However, efforts are required to enhance the production rate to industrial scale because of low production rate in a single droplet generator. In here, we present a method for enhancing production rate of monodisperse droplets via parallelization of flow-focusing generators. For this, we fabricated a three-dimensional monolithic elastomer device (3D MED) that has the 3D channel structures in a single layer, using a double-sided imprinting method. We demonstrated that the production rate of monodisperse droplet is increased by controlling the flow rate of continuous and dispersed phases in 3D MED with 8 droplet generators. Thus, we anticipate that this microfluidic system will be used in wide area including microparticle synthesis and screening system via encapsulation of various materials and cells in monodisperse droplets.
PDMS 블레이드 코팅법을 이용한 종이-기반 바이오센서칩 제작
정헌호 ( Heon-ho Jeong ),박차미 ( Chami Park ) 한국화학공학회 2021 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.59 No.1
This paper proposes the polydimethylsiloxane (PDMS) blade coating method for fabrication of paperbased analytical device (PAD) that is able to monitor the disease diagnosis and progress without special analytical equipment. The mold that has PAD design is easily modified by using laser cutting technique. And the fabricated mold is used for hydrophobic barrier formation by blade coating. We have optimized the stable formation of PDMS hydrophobic barrier as blade coating condition, which is established by analyzing the structure of the PDMS hydrophobic barrier and change of hydrophilic channel size as thickness of the ink and contact time with the chromatography paper. Based on optimal condition, we demonstrate that PAD as biosensor can apply to detect protein, glucose, and metal ion without special analysis equipment.
김지용 ( Gi Yong Kim ),정헌호 ( Heon-ho Jeong ),이창수 ( Chang-soo Lee ),노창현 ( Changhyun Roh ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.2
In this study, we described a simple and facile method to generate uniform microwells poly(dimethyl siloxane) (PDMS) microstamps through micro-molding for efficient, rapid and reliable cell patterning of adipocyte differentiation. In contrast to the conventional methods, the microstamp technologies are low expensive, non-toxic, and using a small amount of solution. Recently, Orlistat known as tetrahydrolipstatin is a prescription drug designed to treat obesity which is used to aid in weight loss and help to reduce overweight obesity. Here, 3T3-L1 cells were treated under various concentration manners of Orlistat 0.2 μM~5.0 μM. and it was confirmed maximum 26.5% inhibition activity compared to control. Thus, we elucidated this platform can be used for the real-time analyzing of cell proliferation, adipocyte differentiation for evaluation of anti-obesity agents on cell chip. Furthermore, we except that this platform technology designed here might be readily be expanded to discover a wider variety of anti-obesity agents.
온도 구배가 있는 미세유체 장치를 이용한 극지 미생물의 형태 변화 분석
정성근(Seong-Geun Jeong),박애리(Aeri Park),정헌호(Heon-Ho Jeong),홍순규(Soon Gyu Hong),이창수(Chang-Soo Lee) 한국생물공학회 2014 KSBB Journal Vol.29 No.4
We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to 40℃ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below 25℃, above 20℃, above 15℃ and above 35℃, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.
액적 기반의 미세유체 시스템을 이용한 초고속 대용량 스크리닝
이창수 ( Chang Soo Lee ),정헌호 ( Heon Ho Jeong ),노영무 ( Young Moo Noh ),장성찬 ( Sung Chan Jang ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.2
Droplet based microfluidic systems have been developed for the application of biological and chemical research field. A picoliter droplet in microfluidic device provides a compartmentalized and well-defined reactor in miniaturized system. The microfluidic system with small droplets can reduce reagent cost and enhance efficiency through automated high-throughput screening system. In this review, we summarize the functionality of droplet based micro fluidic system including droplet generation, precise droplet control, and various applications. In addition, this articlere views current applications in chemistry and biology, and discuss advantages of droplet based microfluidics compared with conventional manner.