http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
고려인삼의 건조 스트레스 처리에 따른 단백질체 비표지 정량분석
정주용(Ju Young Jung),민철우(Cheol Woo Min),신혜원(Hye Won Shin),Truong Van Nguyen,김지현(Ji hyun Kim),김의정(Eui Jung Kim),조익현(Ick Hyun Jo),김유진(Yu Jin Kim),김선태(Sun Tae Kim) 한국약용작물학회 2021 한국약용작물학회지 Vol.29 No.6
Background: Korean ginseng (Panax ginseng Meyer) has long been cultivated as an important medicinal plant. Moderate water loss resulting form drought stress can impairs the growth of ginseng and lead to yield reduction. However, the mechanisms by which drought stress affects ginseng at the proteome level remain pooly undersood. Methods and Results: We carried out label-free quantitative proteomic analysis of ginseng roots subjected to drought stress (grown at less than 10% soil moisture for 2 weeks) and, compared the results with a control samples of ginseng grown at 25% soil moisture. The analysis was carried out using liquid chromatography with tandem mass spectrometry. A total of 2,471 proteins were identified, and 195 of which showed significant modulation. Functional classification revealed that proteins involved in calcium signaling, and photosynthesis and, production of secondary metabolites were enriched in the control sample (cluster_1), whereas proteins associated with stress response redox reaction, electron transport, and protein synthesis were enriched in the drought-stressed root (cluster_2). Conclusions: Our results provide an overview of drought-induced proteomic changes in ginseng root, and illuminate their correlation with physiological changes, revealing potential proteomic markers of drought stress in ginseng.
제올라이트 기반의 향상된 촉매를 사용한 디젤엔진의 NO<SUB>x</SUB> 저감
정주용(Ju Yong Jung),이해수(Hae Soo Lee),김태민(Tae Min Kim),강정호(Jeong Ho Kang),김용태(Young Tae Kim),김상훈(Sang Hoon Kim),김창희(Chang Hee Kim) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
The selective catalytic reduction (SCR) technology is well established and used worldwide to control the emissions of NOx from stationary sources: it can be broadly described as passing a hot exhaust gas over a catalyst in the presence of nitrogenous reductants, such as ammonia or urea. The SCR is also currently employed to reduce NOx contained in the exhaust gases of internal combustion engines operated with excess air, such as Diesel engines. One problem of SCR systems for vehicles is the poor activity at low temperatures where most of the NOx are produced during, e.g. cold start-up and on short travelling distances. A method to increase the efficiency of NOx removal by SCR when the exhaust gas temperature is low is to increase the temperature of the exhaust gas and/or of the SCR catalyst. However, principle solutions of the SCR catalytic activity at low temperature are modification of catalysts which can improve the performance of DeNOx at low temperature and catalytic durability, stability and coat ability. In the present study, we developed and analyzed state-of-the-art catalysts working at low temperature.
김세훈 ( Se-hun-kim ),정주용 ( Ju-young-jeong ),박민호 ( Min-ho-park ),조희제 ( Hee-je-cho ),권기환 ( Gi-hwan Kwon ),정순호 ( Soon-ho Jung ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2
최근 드론 규제를 완화하겠다는 정부의 발표로 앞으로 여러 분야에서 드론이 상용화되어 이용될 것으로 예상되지만, 자율비행에 있어서 장애물을 회피하는 문제가 상용화의 걸림돌로 남아있다. 이에 본 논문에서는 GPS 기반으로 드론을 직접 제작하고, 초음파 센서를 활용하는 회피기동을 구현하여 비교적 저렴한 가격으로 자율비행 드론 시스템을 설계하여 앞으로 나오게 될 스마트 모빌리티 제품군들의 상용화에 기여할 수 있기를 기대한다.
벼의 차세대 단백질체 분석을 위한 질량분석기 호환의 광분해성 계면활성제의 적용
신혜원(Hye Won Shin),응웬반쯔엉(Truong Van Nguyen),정주용(Ju Young Jung),이기현(Gi Hyun Lee),장정우(Jeong Woo Jang),윤진미(Jinmi Yoon),라비굽타(Ravi Gupta),김선태(Sun Tae Kim),민철우(Cheol Woo Min) 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.3
The solubilization of isolated proteins into the adequate buffer containing of surfactants is primary step for proteomic analysis. Particularly, sodium dodecyl sulfate (SDS) is the most widely used surfactant, however, it is not compatible with mass spectrometry (MS). Therefore, it must be removed prior to MS analysis through rigorous washing, which eventually results in inevitable loss. Recently, photocleavable surfactant, 4-hexylphenylazosulfonate (Azo), was reported which can be easily degraded by UV irradiation and is compatible with MS during proteomic approach using animal tissues. In this study, we employed comparative label-free proteomic analysis for evaluating the solubilization efficacy of the Azo and SDS surfactants using rice leave samples. This approach led to identification of 3,365 proteins and out of 682 proteins were determined as significantly modulated. Further, according to the subcellular localization prediction in SDS and Azo, proteins localized in the chloroplast were the major organelle accounting for 64% of the total organelle in the SDS sample, while only 37.5% of organelle proteins solubilized in the Azo were predicted to be localized in chloroplast. Taken together, this study validates the efficient solubilization of total protein isolated from plant material for bottom-up proteomics. Azo surfactant is suitable as substituents of SDS and promising for bottomup proteomics as it facilitates robust protein extraction, rapid washing step during enzymatic digestion, and MS analysis.