돈사상부공간을 이용한 가토사육이 돈의 증체에 미치는 영향 제4보 . 가토분뇨 및 잔사의 자유채식이 사료의 급여수준을 달리했을 때 육성 - 비육돈의 증체에 미치는 영향

정현승,정장용 ( Hyun Sung Chung,Jang Young Jung ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5

This experiment was carried out to determine the effect of liberal feeding of rabbit feces and residual forage on the growth of growing-finishing pigs subject to restricted feeding in which the daily allowance of formula feed was 3 % of body weight, compared with 4 % in the controls. The rabbit feces and residual forage were to be dropped from the hutches above the pig barns. Eight pigs of the same litter gave the similar initial body weight of 40㎏ and were alloted in equal numbers to the control and experimental groups. The results from feeding trial for 2 months are summarized as follows 1. The body weight gain for 2 months was averaged 44.9 ㎏ and 43.0㎏ in the control and experimental pigs, respectively. However, there was no significant (P$gt;0.05) difference in the body gain between two groups. 2. The feed conversion was improved slightly in the experimental group (2.8) as compared with the control (3.4). 3. The palatability of rabbit feces and residual forage to pigs was decreased during the late finishing period. 4. The liberal feeding of rabbit feces and residual resulted in the decrease of about 60 won in the feed cost for 1 ㎏ body weight gain of pigs. 5. The pigs fed rabbit waste and residual forage were found to be normal clinically.

닥나무의 사료가치에 관한 연구 제 2 보 . 닥나무 재배방법 및 엽의 급여가 육성 - 비육돈의 증체에 미치는 영향

정현승,이을희,정장용 ( Hyun Sung Chung,Ell Hee Lee,Jang Young Jung ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5

This experiment was carried out to determine the yield, chemical composition and feeding value of the leaves and rhytidome of Broussonetia kaxirtoici, Sieb. The feeding value of the leaves fed liberally to growing-finishing pigs was determined under the restricted feeding in which the daily allowance of formula feed was 3 % of body weight, compared with 4 % in the controls. Ten pigs of the same litter were alloted in equal numbers into the control and experimental groups and conducted the feeding trial for 2 months. The results obtained are summarized as follows : 1. The annual yield of green leaves of Broussonetia kazinoki, Sieb. grown on the College Experimental Farm was averaged about 2,000㎏/10a. 2. The chemical composition of the leaves was determined on the dry matter basis as follows ; crude protein : 11.4%, crude fat: 4.74%, crude fiber : 32.33%, crude ash : 13.43% and nitrogen free extracts : 37.16%. 3. The chemical composition of the rhytidome was determined on the dry matter basis as follows ; crude protein : 13.43%, crude fat : 5.94%, crude fiber : 33. 19%, crude ash ; 33.60% and nitrogen free extracts : 27.26 %. 4. The body weight gain for 2 months was averaged 33.8㎏ and 36. 2㎏ in the control and experimental pigs, respectively. However, there was no significant (P$lt;0.05) difference in body weight gain between two groups. 5. The feed conversion was improved slightly in the experimental group(3.2) as compared with the control (3.8). 6. The liberal feeding of the green leaves resulted in the decrease of about 60 won in the feed cost for 1 ㎏ body gain of the growing-finishing pigs. 7. The leaves were found palatable to the pigs and the pigs fed the leaves were found to be normal clinically.

소 난포란의 체외성숙도가 수정 및 체외발달에 미치는 영향

정장용,박희성,박성진,문승식,정현승 진주산업대학교 농업기술연구소 1995 農業技術硏究所報 Vol.8 No.-

본 연구소는 소 난포란을 이용하여 체외수정시에 체외성숙 시간이 체외수정 및 체외발달에 미치는 영향을 조사하고자 실험을 수행하였다. 도살장에서 도살된 한우의 난소를 채취하여 실험실로 2시간 이내에 운반하여 난소를 깨끗이 세척한 후 18 gauge needle로 난포의 크기별로 난포란을 회수하여 10% FCS가 첨가된 TCM-199 배양액에 FSH(35 ㎍/ml), LH(10㎍/ml), estradiol-17β(1㎍/ml)가 첨가된 체외성숙용 배양액에서 20-28시간 동안 체외성숙을 유도하였다. 체외성숙란을 한우 동결정액을 이용하여 체외수정을 시킨 후 난관상피세포에서 공배양을 실시하여 수정란의 체외발달을 유도하였으며, 난포란의 등급별 회수율과 체외성숙시간에 따른 수정율 및 체외발달율을 조사한 결과는 다음과 같다. 난소에서 난포의 크기에 따른 Grade I의 회수율은 소, 중 및 대난포에서 각각 31.3, 27.8 및 3.7%였으며, 48개의 난소로 부터 모두 398개의 미성숙 난자를 회수하여 난소당 8.3개의 미성숙 난자를 회수하였다. 등급별로는 2.3, 2.5, 2.3 및 1.2%의 회수율을 보였다. 체외수정란의 배반포로의 발달율은 24시간 성숙시켰을 때 27.2%로서 20(11.6%) 및 28(9.0%)시간 성숙시켰을 때 보다 유의적(P<0.05)으로 높았다. 그러나 22, 24 및 26시간 성숙시켰을 때는 성숙시간에 따른 발달율은 유의적(P<0.05)인 차이가 없었다. The ovaries of Korean native cow or heifers were obtained from an abattoir and kept on 28-30℃ and transported to laboratory within 2 hrs. The follicular oocytes were collected from small, medium and large follicles, and classified into 3 grades by the morphology of cumulus cells attached and homogenous cytoplasm. The oocytes were matured in vitro for 24 hrs. in TCM-199 supplemented with 35㎍/ml FSH, 10㎍/ml LH, 1㎍/ml estradiol-17β and granulosa cells at 39℃ under 5% CO_2 in air. They were fertilized in vitro by freezing sperm treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro with bovine oviductal epithelial cells for 7 to 9 days. The follicular oocytes recovered were classified into 41.7% as gradae Ⅰ, 51.5% as grade Ⅱ and 6.8% as grade Ⅲ. The number of oocytes recovered per ovary was averaged 8.3 and they were classified into 2.3 as grade Ⅰ, 2.5 as grade Ⅱ and 2.3 as grade Ⅲ. The cleavage rates of the embryos obtained from 73.3, 78.4, 80.4, 77.3 and 72.7% in the groups of maturation age of 20, 22, 24, 26 and 28 hours respectively. The in vitro culture of bovine embryos with maturation age from 22-26 hours improved significantly(P<0.05) the subsequent development to transferable embryos(20.4-25.0%), compared with 20 hours(11.6%) or 28 hours(9.0%).

소 난포란의 체외수정에 있어서 정액의 처리방법이 수정 및 체외발달에 미치는 영향

정현승,정장용,박희성 진주산업대학교 농업기술연구소 1996 農業技術硏究所報 Vol.9 No.-

본 연구에서는 도살장에서 도살되는 한우의 난소를 적출하여 난소로부터 미성숙 난포란을 회수하여 체외에서 성숙한 난자를 동결정액과 정소상체미부 정액을 처리 방법에 따른 수정 및 체외발달에 미치는 영향을 조사하고자 본 실험을 수행하였다. 도살장에서 도살된 한우의 난소를 채취하여 체외성숙을 시킨 다음 한우 동결정액을 융해하여 B. O.배양액으로 원심분리를 2∼3회 반복하여 동해방지제를 제거한후 CO_2 incubator에서 0∼2시간 동안 swim-up을 유도하였다. 정소상체미부정자는 도축장에서 도살 직후 정소를 채취하여 체외수정에 사용하였다. 체외수정은 수정용 B. O.배양액 80∼100ul 당 10∼15개의 난자를 옮겨 정자의 최종농도가 2×10 exp (6) sperms/ml이 되게 첨가하여 약 24시간동안 CO_2 incu bator에서 수정을 유도하였다. 체외수정이 이루어진 수정란을 회수하여 10% FCS가 첨가된 TCM-199 배양액으로 4-5회 세척하여 monolayer를 형성하고 있는 난관상피세포와 공배양을 시키면서 48시간 간격으로 신선한 배양액으로 교환하면서 수정란의 체외발달을 유도한 결과는 다음과 같다. 체외수정율은 정소상체미부 정자를 사용하였을 때가 66.1%로서 동결정액을 사용하였을 때의 51.3% 보다는 유의적(P<0.05)으로 높았으며, 배반포로의 발달율은 각각 19.6 및 14.3%으로서 이들간에 유의적(P<0.05)인 차이가 없었다. swim-up처리 시간에 따른 수정율은 59.3∼63.4%의 수정이 이루어져 swim-up처리 시간에 따른 수정율은 유의적(P<0.05)인 차이가 없었으며, 체외수정란의 배반포로의 발달율도 13.6∼19.3%가 배반포로 발달하였으나 이들간에 유의적(P<0.05)인 차이는 없었다. The ovaries of Korean native cow or heifers were obtained from a slaughter house and kept on 28∼30℃ and transported to laboratory within 2 hrs. The follicular oocytes were collected follicles. The oocytes were matured in vitro for 24 hrs. in TCM-99 supplemented with 35ug/ml FSH, 10ug/ml LG, 1ug/ml estradiol-17 and granulosa cells at 39℃ under 5% CO_2 in air. The caudal epididymis of Korean native bulls were obtained from a slaughter house and transported to laboratory within 30 minutes. Swim-up of collected spermatozoa and Freezing sperm was layered under 2ml fertilization B. O. medium in two tissue culture tubes and held at a 45℃ angle for 0∼2 hour. They were fertilized in vitro by freezing sperm treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro with bovine oviductal epithelial cells for 7 to 9 days. The cleavage rate of matured oocytes was significantly(P<0.05) higher after in vitro fertilization in caudal epididyms(66.1%) than freezing sperm(513%). Similarly, the proporation of cleaved zygotes that developed to blastocysts was 19.6% and 14.3% respectively. The cleavage rate and in vitro developmental rate in swin-up period of freezing sperm was similar(59.3∼63.4% and 13.6∼19.3%).