Establishment of Dyeing Data for Silk Fabrics and Cells Using Diospyros kaki Thunb

정석률 한국사물인터넷학회 2023 한국사물인터넷학회 논문지 Vol.9 No.3

In this study, it was analyzed with the dyeing pattern of Diospyros kaki Thunb (persimmon) and was tried to numerically evaluate how the dyeing pattern in silk fabrics and cells was changed by different mordants. When the dyed silk fabrics were sufficiently dried, the silk fabrics were found to have a pale yellow color. Interestingly, iron II sulfate mordant changed the color change the most, silk fabrics were dyed with a color close to brown or dark purple. For numerical analysis, 19% and 62.5% color changes could be induced by sodium tartrate plus citric acid and copper acetate, respectively. Iron II sulfate induced the greatest difference than that of untreated mordants at 88%. About 5% and 10% of Chinese hamster ovary (CHO) cells were stained by sodium tartrate plus citric acid and copper acetate, respectively. The staining effect induced by iron II sulfate was about 2.4 times higher than the staining effect by sodium tartrate plus citric acid. In previous studies, staining results have been visually confirmed. However, this results not only visually confirmed the dyeing, but also quantified the color change. In particular, if numerical results are continuously integrated into big data, any researcher will be able to easily obtain similar results even if the method, time, volume, etc. are changed. In addition, the numerical data of this study is considered to be an important basis for building a database for IoT construction and computer analysis.

콘택트렌즈 세척액이 숙주세포와 가시아메바에 미치는 영향

정석률 남서울대학교 2014 남서울대학교 논문집 Vol.S No.-

Acanthamoeba is opportunistic protozoan pathogen causing keratitis and encephalitis in human and experimental animals. Subsequent invasion of the connective tissue and induction of pro-inflammatory responses leads to neuronal damage that can be fatal within days. In this study, lens-clearing solutions were treated to Acanthamoeba castellanii and human brain microvascular endothelial cell (HBMEC) and cytotoxicity was analyzed. When the lens-clearing solutions was treated to the amoeba, the amoeba were changed into round form of no lightness. Cytotoxicity by the lens-clearing solutions of "Eyetec RGP Care (A)" and "Biotrue (B)" were decreased to 14.2% (A) and 30.8% (B), respectively. HBMEC cytotoxicity by the lens solution A and B were similar when compared with a negative control without the lens-clearing solutions. Amoeba when compared lens solution A and B, lens solution B is more effective. Key Words : Lens-Clearing Solution, Acanthamoeba, Eyetec RGP Care, Biotrue, Human brain microvascular endothelial cell

가시아메바 병원성의 이해

정석률 남서울대학교 2010 남서울대학교 논문집 Vol.16 No.1-1

가시아메바 (Acanthamoeba)는 단세포 진핵 원생생물이며 환경에서 자유 생활한다. 인체와 실험동물에서 만성 육아종성 수막뇌염과 아메바성 각막염을 유발하여 사망과 실명을 각각 초래할 수 있다. 특히, 만성 육아종성 수막뇌염은 면역이 저하된 환자나 면역억제제를 투여받고 있는 환자에게서도 발병이 가능하다. 본 연구에서는, 표적세포와 가시아메바의 상호작용 및 병원성을 유발하는 인자를 살펴보았으며 이것으로 가시아메바의 병원성을 이해하고자 하였다. 가시아메바는 주로 비강을 통하여 후신경을 경유하여 뇌-혈관 경계막을 뚫고 뇌에 감염된다. 여러 표적세포 중 소교세포와의 상호작용이 많은 관심이 대상이 되어왔는데, 이는 면역세포이면서 가시아메바를 사멸하기도 하며 가시아메바 또한 소교세포 등을 공격하여 사멸을 유도한다. 이것은 가시아메바의 감염정도와 병원성의 정도에 의존 할 것을 사료된다. 또한, 아메바성 각막염은 콘텍트렌즈를 착용하는 사람에게서 주로 발병되며, 원인은 비위생적인 세척 및 보관에의하여 콘텍트렌즈 보관용기에 가시아메바가 성장하여 눈으로 가서 발병된다. 매우 치명적이며 심할 경우 각막염을 유발하여 실명에까지 이르게 한다. 본 연구에서는, 가시아메바 감염에 의한 수막뇌염과 콘텍트렌즈를 착용하는 사람에게 경고를 해 줄 수 있는 자료로 제공될 것이며 향후, 백신이나 치료제를 개발하기위한 기초연구로써 활용되길 기대하고 있다. 핵심어: 가시아메바, 만성 육아종성 수막뇌염, 아메바성 각막염

사다리운동을 이용한 감각운동훈련이 남자대학생의 뇌기능관련 호르몬과 동적균형에 미치는 영향

정석률,이해림,이성기 한국스포츠학회 2021 한국스포츠학회지 Vol.19 No.4

본 연구의 목적은 사다리운동을 이용한 감각운동훈련을 적용하여 뇌기능 관련 호르몬의 발현 및 동적 균형에 미치 는 효과성을 과학적으로 검증하는데 있다. 연구대상은 남자 대학생 30명으로 감각운동집단(SMTG), 트레드밀운동집단 (TEG), 통제집단(NEG)으로 반복측정하였다. 이에 따른 결과 첫째, 뇌기능 관련 호르몬의 BDNF는 감각운동훈련집단 이 트레드밀 운동집단과 통제집단보다 유의하게 증가하였다. IGF-1은 운동 전후 비교 시 감각운동 훈련집단과 트레드밀 운동집단에서 유의한 증가를 나타내었다. 둘째, 동적균형의 변화는 감각운동훈련집단에서 오른발과 왼발의 후내측, 후외 측, 정면이 유의하게 나타났다. 이러한 결과를 바탕으로 사다리를 이용한 감각운동훈련 적용이 BDNF, IGF-1, 동적균형 변화에 긍정적인 영향을 나타낸다. The purpose of this study is to scientifically verify the effect of sensorimotor training using ladder exercise on the expression and dynamic balance of brain function-related hormones. The subjects of the study were 30 male university students, and the repeated measures ANOVA in the sensorimotor group(SMTG), treadmill motor group(TEG), and non control group(NEG). As a result, first, BDNF, a brain function-related hormone, was significantly increased in the sensorimotor training group than in the treadmill exercise group and the control group. IGF-1 showed a significant increase in the sensorimotor training group and the treadmill exercise group when compared to before and after exercise. Second, changes in dynamic equilibrium were significantly present in the postero-medial, postero-lateral, and anterior sides of the right and left feet in the sensorimotor training population. Based on these results, the application of ladder-based sensorimotor training has a positive effect on changes in BDNF, IGF-1, and dynamic balance.

Usefulness of Chlorine Dioxide to Airborne Bacteria at a Hospital Using Biological Information

정석률 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.2

In the present study, using biological information of bacteria and biochemical information of chlorine dioxide gas, Gram-positive bacteria, e.g., Alloiococcus otitis, Erysipelothrix rhusiopathiae, Staphylococcus caprae, Staphylococcus lentus, and gram-negative bacteria, e.g., Acinetobacter baumannii complex, Aeromonas salmonicida, Brucella melitensis, Oligella ureolytica were used whether a plastic kit to release ClO2 gas could inhibit their growth. Overall, chlorine dioxide gas showed about 99% inhibition of bacterial growth, with less than 10 CFU. However, it was found that Gram positive Alloiococcus otitis and Gram negative Aeromonas salmonicida had more than about 50 CFU. When comparing the results of experiments with several bacteria, it suggested that the concentration of chlorine dioxide gas would be at least 10 ppm to 400 ppm for the bacterial inhibition. The results of this study could be used as basic data to evaluate the clinical usefulness of chlorine dioxide gas. If this study helps with prior knowledge to help clinicians to recognize and prevent the presence of micro-organisms that cause infections in hospitals, it would be helpful for activities such as patient care as a convergence field. In the future, it is considered that the research results will be the basis for rapidly inhibiting the microbes infected with patients by utilizing data of the information of the microbes that are inhibited for chlorine dioxide gas.

Construction of Dyeing Condition System for Lithospermum erythrorhizon by Applying Natural Dye and Mordants

정석률,Jung, Suk-Yul The Korea Internet of Things Society 2020 한국사물인터넷학회 논문지 Vol.6 No.4

2007년에 천연 염료 관련 자원, 색상, 염색 원단 등 천연 염색 정보를 쉽게 제공 할 수 있도록 모바일 어플리케이션을 설계 한 것으로 알려졌다. 천연 염색과 IoT의 연관성, 응용 등에 대한 연구가 아직 부족한만큼 다양성 천연 염색에 의한 염색 패턴 변화에 관한 정보가 필요하다. 본 연구에서는, 천연 염료, 예를 들어 Lithospermum erythrorhizon가 한국에서 전통적으로 많이 사용되어온 실크에 대하여 염색 정보를 구축하고자 했다. L. erythrorhizon에서 염료의 추출은 pH4에서 수행되었다. L. erythrorhizon의 건조 된 뿌리는 짙은 갈색을 띤 자주색을 보였다. 매염제가 없는 실크 직물은 일반적으로 보라색 염색 패턴을 보였다. 타르타르산 나트륨과 구연산으로 염색 한 실크 직물은 투명한 갈색으로 염색되었다. 흥미롭게도 황산 철 (II)의 매염제 인 실크 직물은 검은 색이 아닌 밝은 회색으로 염색되었다. 알루미늄 포타슘 설페이트의 매염제를 L. erythrorhizon 추출 염료로 처리 한 결과 매염제를 처리하지 않았을 때와 거의 동일했다. 염색도를 수치로 평가 한 결과, 중크롬산 칼륨의 매염제의 처리는 약 50% 어둡고, 황산 철 (II)에 의한 염색은 약 75% 어두웠다. 이러한 결과는 L. erythrorhizon을 이용한 다양한 염색 색 적용 연구에 도움이되며, 염색 정도와 pH 농도, 색도변화라는 염색변수에 의한 염색 컨트롤러와 데이터베이스 시스템 구축에관한 정보를 제공 할 것이다. It was reported that a mobile application was designed to easily provide natural dyeing information such as natural dye related resources, colors and dyed fabrics in 2007. Since studies on the linkage, application, etc. between natural dye dyeing and IoT are still lacking, diversity of information on the change of dyeing pattern by natural dye dyeing is required. In this study, it was to construct dyeing information by natural dyes, e.g., Lithospermum erythrorhizon, on silk, which has been traditionally used as many fibers in Korea. The extraction of the dye from L. erythrorhizon was carried out under pH4. The dried root of L. erythrorhizon showed dark brownish purple. Silk fabric by a without a mordant typically showed a purple dyed pattern. In the staining by sodium tartrate plus citric acid, silk fabric was stained clear brown. Interestingly, the mordant of iron (II) sulfate, the silk fabric was dyed in a light gray color rather than black. When the mordant of aluminum potassium sulfate was treated with L. erythrorhizon-extracted dye, the results were almost the same as when the mordant was not treated. When the degree of dyeing was evaluated numerically, the treatment of the mordant of potassium dichromate was about 50% darker, and the dyeing by iron (II) sulfate was about 75% darker. These results will be helpful in the study of applying various dye colors using L. erythrorhizon, and it will provide information on dyeing controller and database system construction by dyeing parameters such as dyeing degree, pH concentration, and chromaticity change.

Mordants Application and Data Establishment for Natural Dye Standardization and Accuarcy

정석률,Jung, Suk-Yul The Korea Internet of Things Society 2021 한국사물인터넷학회 논문지 Vol.7 No.4

천연염색은 전통적으로 세계 여러 나라에서 사용되어 왔으며, 천연염색이 다양해짐에 따라 염색 패턴의 다양성도 확대되고 있다. 본 연구는 4가지 천연염료에 대한 염료와 매염제의 색상 변화를 보다 정확하게 분석하여 사물인터넷에 정량화된 정보를 제공할 수 있는 수치를 제공함으로써 표준화를 마련하고자 했다. Juglans regia Linn에서 추출한 염료에 구리 아세테이트, 철 II 황산염 및 중크롬산 칼륨을 첨가하면 갈색의 원래 색상이 각각 보라색, 카키색 및 암갈색의 다른 색상으로 변경되었다. Sophora japonica L. 또는 Phellodendron amurense Ruprecht에 첨가된 중크롬산 칼륨을 제외하고는 다른 매염제의 농도는 감소되었지만 염색된 실크의 색상 차이는 매우 컸다. 그러나 정도의 차이는 있지만 초산구리와 황산철은 각각 35%와 15%의 색변화를 유도하였다. 종합해볼때, J. regia Linn, S. japonica L.과 P. amurense Ruprecht에는 copper acetate 15 gram을 Phytolacca americana에는 150 gram의 iron이 정확히 첨가된 것이 가장 높은 색상변화를 유도할 수 있다고 확인되었다. 본 연구의 결과는 다양한 매염제에 의한 정확한 색상 변화가 염료 및 매염제에 의한 보다 정확한 색상 유도를 가능하게 하는 중요한 정보로 활용될 수 있음을 시사하였다. Natural dyeing has traditionally been used in many countries around the world, and as natural dyes are diversified, the diversity of dyeing patterns is also expanding. This study tried to establish standardization by providing numerical values that could provide quantified information to the Internet of Things by more accurately analyzing the color changes of dyes and mordants for the four natural dyes. The addition of copper acetate, iron II sulfate and potassium dichromate to the dye extracted from Juglans regia Linn changed the original color of brown to other colors of purple, khaki and dark brown, respectively. Except for potassium dichromate added to Sophora japonica L. or Phellodendron amurense Ruprecht, the concentration of other mordants was reduced, but the color difference of the dyed silk was very large. However, although there is a difference in degree, copper acetate and iron sulfate induced color changes of 35% and 15%, respectively. In summary, it was confirmed that the highest color change was induced when 15 grams of copper acetate was added to J. regia Linn, S. japonica L. and P. amurense Ruprecht and 150 grams of iron to Phytolacca americana. The results of this study suggested that the accurate color change by various mordants can be utilized as important information that enables more accurate color induction by dyes and mordants.

Safety Analysis of Solutions for Washing of Contact Lenses

정석률 한국엔터테인먼트산업학회 2014 한국엔터테인먼트산업학회논문지 Vol.8 No.3

Acanthamoeba is a free-living protozoan pathogen which causes blinding amoebic keratitis (AK) to contact-lens users. In this study, using commercialized contact lens-clearing solutions, their cytotoxicity were analyzed against Acanthamoeba. Moreover, their effects to Acanthamoeba were compared with cytotoxicity to a human corneal epithelial cell (HCEC) as a target cell. Only 10% of the lens solution of Amo Total Care 1 didn't significantly affect the morphology of A. castellanii. As the percent of the lens solution was increased, it decreased the number of A. castellanii and changed shapes of the amoebae. 10% and 50% of Opti-free RepleniSH decreased the number of A. castellanii more than Amo Total Care 1. 0 to 100 ul of lens solutions had little cytotoxicity to HCEC. A. castellanii was severely affected about 40% of cytotoxicity of Amo Total Care 1. On the other hand, Opti-free RepleniSH had about 38% to 58% cytotoxicity to A. castellanii Our results showed that two contact-lens clearing solutions didn't have very strong effects to A. castellanii trophozoites. Therefore, it suggests that other compositions and compounds of stronger cytotoxicity should be developed for disinfections to A. castellanii.

줄사다리를 이용한 기능적 운동이 남자대학생의 뇌파 활성에 미치는 영향

정석률,이해림,이성기 한국자연치유학회 2022 Journal of Naturopathy Vol.11 No.2

대한민국 남성에서 C-반응성 단백질과 관련된 PLA2G4A 유전자의 다형성

정석률 한국엔터테인먼트산업학회 2013 한국엔터테인먼트산업학회논문지 Vol.7 No.3

The PLA2G4A catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid, which is lipid-based cellular hormones that regulate inflammatory response. The C-reactive protein in blood can be influenced by inflammation, infection, trauma and tissue necrosis etc. We performed the association analysis between the C-reactive protein level in blood and the 19 SNPs in the PLA2G4A gene in the Korean Association Resource (KARE) cohort (839eldermales). The 3 SNPs (rs4651330, rs12720526 and rs16826049) in the PLA2G4A gene showed a significant association with C-reactive protein (p-values:0.0015~0.025). This study provides additional evidence in support of the finding that the PLA2G4A gene maybe related to infection of microbes. This study may provide insight into the genetic basis of C-reactive protein related to reaction of infection.