중금속의 독성 기전에 관한 연구 (Ⅰ)

정명규(Myung Kiu Chung),임경택(Gyong Taek Lim),정현옥(Hyun Ok Jung) 大韓環境工學會 1994 대한환경공학회지 Vol.16 No.5

수서생태계 납오염에 대한 생체지표로서 Maceobrachium nipponnese내 Na+/K+ATPase의 활용

정명규 ( Myung Kiu Chung ),김학희 ( Hak Hee Kim ) 한국물환경학회 2002 한국물환경학회지 Vol.18 No.4

Lead is one of the most toxic metal and is detectable in practically all phases of environment and in all biological system. Transport, industrial and domestic waste products are the main sources of this pollutant, Ingested lead is rapidly absorbed and widely distributed throughout the body, causing extensive tissue damage. In this study, we chose the freshwater decapods Maceobrachium nipponnese as a sensitive indicator organism for environmental pollution. In order to investigate the possibility in use of Na^+/L^+ ATPase activity as a biomarker of lead pollution. we tested the acute toxicity of lead to Maceobrachium nipponnese. The LC_50(96hr) value for lead in Maceobrachium nipponnese was found to be 446㎍/L with the 95% confidence limits. The lead exposure group at LC_50 showed a significant Na^+/K^+ATPase inhibition, depending on the exposure time. Comparision of several concentrations of lead revealed that the Na^+/K^+ATPase activity in Maceobrachium nipponnese was significantly decreased in a concentration dependent manner. These results suggest that Na^+/K^+ATPase activity in Maceobrachium nipponnese may possibly be used as a biomarker of lead pollution in aquatic ecosystem.

The Mechanism of Hematoxylin on Glucose Metabolism Improvement in Sciatic Nerves from Streptozotocin-induced Diabetic Rats.

정명규(Chung, Myung-Kiu),강순국(Kang, Soon-Kuk) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.7

Hematoxylin은 한국의 남부지방에서 당뇨합병증을 치료하기 위하여 사용해오던 민간약제인 Hematoxylon campechianum의 주성분이다. 본 논문에서는 hematoxylin의 혈당저하 메커니즘을 연구하기 위하여 3개 군의 흰쥐-정상군, 당뇨군, hematoxylin 처치군-에서 분리한 대퇴신경을 대상으로 2-deoxyglucose 수송능과 인지질 대사능을 조사였다. 실험 결과 hematoxylin은 당뇨군에서 혈당치를 현저하게 감소시키는 것으로 나타났다. 또한 무게단위의 기준으로 당뇨군의 신 경조직의 경우, 총 인지질의 양은 20% 감소하였으나 상대적으로 phosphatidylinositide의 감소는 작은 것으로 나타났다. 이 경우, hematoxylin을 처치하면 2-[3H] myo-inositol이 총 phosphoinositids로 대사되는 비율이 증가하는 것으로 밝혀졌 다. 이러한 효과는 낮은 농도의 hematoxylin 처치군보다 높은 농도의 처치군에서 훨씬 큰 것으로 나타났다. 이상의 실험결 과는 hematoxylin의 당뇨개선 메커니즘이 myo-inositol 대사를 증가시킴으로써 체내 당 수송과 인지질 대사를 정상화시킨 다는 점을 제시하고 있다. 따라서 본 연구결과를 토대로 저자들은 hematoxylin은 향후 당뇨 치료제로서 후보 물질이 될 수 있음을 제시하고자 한다. Hematoxylin is the main component of Hematoxylon campechianum which has been utilized in the southern provinces of Korea as a folk remedy for diabetic complications. In the present study, to investigate the hypoglycemic mechanism of hematoxylin, the 2-deoxyglucose uptake and phospholipid metabolism were examined in sciatic nerves from three groups of rats : normal control, diabetic control, diabetic hematoxylin-treated group. Hematoxylin significantly reduced blood glucose levels in diabetic control rats. On a wet weight basis, the nerves from diabetic rats showed a 20% decrease in total phospholipid from that of controls and a relative decrease in phosphatidylinositide. Hematoxylin treatment increased the incorporation rate of 2-[3H] myo-inositol into total phosphoinositids in diabetic rat. The effectiveness were more potent in higher dose hematoxylin-treated rats than lower dose hematoxylin-treated rats. These results suggest that hematoxylin increases glucose transport and lipid metabolism by partially normalizing concerned with myo-inositol metabolism in diabetic rat. Therefore we propose that hematoxylin can be a promising candidate for diabetes medication.

납이 HL 60 세포 내 Myo-inositol 수송 체계에 미치는 영향

정명규(Myung Kiu Chung) 한국환경보건학회 1999 한국환경보건학회지 Vol.25 No.2

Lead is the most ubiquitous toxic metal and released to the environment from natural and man-made sources. Presence of lead in the environment has led to an increasing awareness and concern of deterimental effect on ecosystem and human health. But there are several limits for toxicity assessment and protection human from toxicity of lead because the toxic effects of lead are detectable in practice only at high exposure concentraion. Therfore, it is very important to search sensitive biomakers at low exposure concentration which could not be observed any practical symptoms such as anemia, encephalopathy, and neuropathy in human. In the present study, to investigate myo-inositol concentration in cell could be a biomaker for lead cytotoxicity, the toxic effects of lead on myo-inositol uptake system were studied in the HL 60 cell line from three groups : normal control, lOppm-lead treated group, 80 ppm-lead treated group. Lead significantly reduced specific myo-inositol uptake in lead-treated groups, but did not affected nonspecific uptake. The magnitude of reduction in myo-inositol uptake was directly propotional to exposure dose. But myo-inositol uptakes in lead treated groups were significantly normalized by addition sodium ion to culture medium. These results suggest that myo-inositol concentration in the cell could be a useful biomaker in toxicity test with cell line and the toxic mechanism of lead on HL 60 cell might be a reduction in myo-inositol uptake, which induced by the perturbation of the sodiumion metabolism.

Molybdenum이 납 중독 랫드의 말초신경내 myo-inositol uptake 시스템에 미치는 영향

송진호(Jin-Ho Song),정명규(Myung-Kiu Chung) 대한환경위생공학회 2003 대한환경위생공학회지 Vol.18 No.2

This study was carried out to elucidate the preventive mechanism of molybdenum on lead-induced neuropathy. An animal model of lead neuropathy was induced by feeding diet containing lead to Sprague-Dawley rat for three weeks. Four weeks aged Sprague-Dawley rats were divided into four groups: normal control group. 10ppm-lead treated group. Img/kg-molybdenum treated group, 10ppm-lead and 1mg/kg-molybdenum treated group. The parameters on neuropathy were examined by measuring concentrdtion of myo-inositol and myo-inosito uptake in sciatic nerve. In the lead-treated rats, myo-inositol concentration and myo-inositol uptake rate were reduced by from 54% to 33% respectively. This deficit results from that myo-inositol uptake system which is canier mediated and sodium-potassium dependent was inhibited by the lead treatment. However, the molybdenum administration significantly eliminated the impairment and maintained myo-inositol concentration to about 82% of normal level. These results suggest that lead-induced neurotoxicity was significantly reduced by administration of molybdenum and the mechanism might be partly normalization of myo-inositol uptake system in sciatic nerve.

폭발 단결정과 합성 다결정 나노다이아몬드의 물리화학적 특성 비교

강순국(Kang, Soon-Kuk),정명규(Chung, Myung-Kiu) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.10

나노다이아몬드는 넓은 적용성 측면을 고려한다면 상대적으로 신물질이라 할 수 있다. 본 연구에서는 XRD, EDS, HRTEM, FTIR, Raman 분광기, 열분석 장치와 BET 측정 장치와 같은 다양한 장비를 통해 단결정 폭발 나노다 이아몬드와 다결정 합성 나노다이아몬드의 물리화학적 특성들을 고찰하였다. 단결정 폭발 나노다이아몬드는 4nm ~ 6nm 크기의 구형이나 타원 모양이지만, 다결정 합성 다이아몬드는 80nm ~ 120nm 크기의 각이 진 입체도형 모양이 다. 단결정과 다결정 나노다이아몬드의 표면은 수산기, 카보닐기, 카복실기 등과 같은 다양한 관능기를 가지고 있다. 질소분위기 하에서 단결정 폭발 나노다이아몬드의 상전이 온도는 대략 650℃이었다. Nanodiamond is a relatively new nanomaterial with broad prospects for application. In this paper, a variety of methods were used to analyze comprehensively chemicophysics properties of the detonation monocrystalline and synthetic polycrystalline nanodiamond, XRD spectroscopy, EDS, HRTEM, FTIR, Raman spectroscopy, TGA-DTA and BET. The results show that the monocryctalline detonation nanodiamond particles are spherical or elliptical shape of 4nm ~ 6nm grain size and the polycryctalline synthetic nanodiamond particles are angular shape of 80nm ~ 120nm grain size. The surface of the monocrystalline and polycrystalline nanodiamond contain hydroxy, carbonyl, carboxyl, ether-based resin, and other functional groups. The phase transition temperature of the monocrystalline detonation nanodiamond in the <I>N</I>2 is about 650 °C.

나노 다이아몬드의 UV차단 효과

강순국(Kang, Soon-Kook),정명규(Chung, Myung-Kiu) 한국산학기술학회 2010 한국산학기술학회 학술대회 Vol.- No.-

대기 영역을 통과한 UV는 인간과 자연 환경 및 재료 등의 합성 물질에 대해 심각한 피해를 유발할 수 있다. 폭발과 산처리된 나노 다이아몬드 표면의 곁가지 결합인 결합체 내의 알킬기에 의한 광흡수 및 산란으로 UV을 차단할 수 있다. 본 연구에서는 폭발 및 산처리 다이아몬드와 나노급 흑연의 농도, 조성에 따른 수용성 현탁액에 의한 차외선 차단 효과를 조사하였다.

수산화 나트륨과 수산화 칼륨 활성화에 의한 왕겨활성탄 제조

김학희 ( Hak Hee Kim ),이주명 ( Joo Myung Lee ),정명규 ( Myung Kiu Chung ) 한국공업화학회 2003 공업화학 Vol.14 No.4

활성탄은 높은 흡착용량으로 인해 가스정제, 수질정제, 촉매나 촉매의 담체, 분리공정 등에 공업적으로 널리 응용되고 있다. 본 논문에서는 왕겨를 원료로 하여 NaOH KOH로 화학적 활성화하여 활성탄을 제조하였다. 활성탄은 BET 비표면적, N_2 등온흡착, 세공크기분포, 평균세공반경, 세공용적, SEM 관찰을 통해 그 특성을 조사하였다. 특히 화학적 활성화에 의한 활성탄 제조에 있어서 중요한 인자는 침적비율임을 알 수 있었다. NaOH, KOH을 이용한 화학적 활성화법에 의해 제조된 활성탄의 비표면적은 610~1342 m^2/g, 총세공용적은 0.34~l.12 cm^2/g, 세공반경은 32~71 Å으로 각각 나타났다. 왕겨를 원료로 화학적 활성화법에 의한 활성탄의 제조는 성공적으로 수행되었다. Owing to its high adsorption capacity, activated carbon has been used widely in industrial application such as purification of gas and water, catalyst, catalyst support and separation process. In this paper, activated carbon was prepared from rice hull by chemical activation with NaOH and KOH. The characteristics of activated carbon through BET specific surface area, N_2 adsorption isotherm, pore size distribution, average pore radius, pore volume and SEM photograph were examined. The results showed that the most important factor in the preparation of activated carbon by chemical activation was the ratio of chemical agents added. The N_2-BET specific surface area, total pore volume and average pore radius by chemical activation with NaOH and KOH were 610~1342 m^2/g, 0.34~l.12 cm^3/g and 32~71 Å, respectively. Thus the preparation of activated carbon from rice-hull by the chemical activation was successfully carried out.

Study on phase inversion parameters of PLGA membrane

Yuying Xie(시에위잉),Jong-Soon Park(박종순),Myung-Kiu Chung(정명규),Soon-Kook Kang(강순국) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-

The PLGA membrane with interconnected pores was formed by phase inversion. According to the membrane surface and cross section pore (SEM image), inter pore was linked. The measured mechanical strength of the membrane was decreased with a membrane thickness. The pore size and porosity of PLGA membrane was decreased with a content of PLGA.

랫드의 대퇴 신경중 ATPase 효소활성에 미치는 납의 영향

정명규 한국환경독성학회 1994 환경독성보건학회지 Vol.9 No.1

Nerve conduction impairment in lead neuropathy has been empirically linked to altered nerve myo-inositol metabolism. In most cases of neuropathy, abnormal myo-inositol metabolism is associated with abnormal Na^(+)/K^(+) ATPase provides a potential mechanism to relate defects of the myo-inositol metabolism in the peripheral nerve treated with lead. Therefore, the effect of lead on the rat sciatic nerve Na^(+)/K^(+) ATPase and other ATPase of sciatic nerve was studied. ATPase activity was measured enzymatically in sciatic nerve homogenates from 2-wk lead treated neuropathy rats and age-mached controls administered myo-inositol. Na^(+)/K^(+) ATPase components were assessed by ouabain inhibition or the omission of sodium and potassium ions. Lead reduced 50% reduction in the Na^(+)/K^(+) ATPase activity in homogenates of sciatic nerve. The 50% reduction in the Na^(+)/K^(+) ATPase activity was selectively prevented by myo-inositol treatment. This study suggests that the toxic mechanism of the lead on peripheral nerve may be through reduction in Na^(+)/K^(+) ATPase activity which has been linked to axonal transport slowing in the rat model of lead neuropathy, via direct changes by the perturbation of the intracelluar sodium or potasium level.