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장판식,서현미,권오택,이현규,김영석 한국식품과학회 2004 Food Science and Biotechnology Vol.13 No.2
Primary alcohol group of 1-monostearoyl glycerol (1-MS) was oxidized using 2,2,6,6-tetramethyl-1-piperidineoxoammonium ion (TEMPO)/sodium hypochlorite/sodium bromide at 25oC and pH 10.8. Regio-selective oxidation of primaryalcohol without oxidation of secondary alcohol group was confirmed by chemical shift at 175 ppm and no chemical shiftsbetween 198-205 ppm in 13C-NMR spectra, and a sharp peak at 1,600 cm?1 an IR spectra. Water solubility and dispersibility ofthe oxidized 1-MS increased from 0.5 to 7.3 mg/mL and from 42 to 90%, respectively. Emulsion formed by oxidized 1-MShad higher emulsion stability. Results revealed that oxidized 1-MS is more suitable for preparation of emulsion containinghigh concentration of water than for that containing oil.
朴官和,張判植 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.1
Fusarium moniliforme으로부터 酵母細胞壁 分解酵素의 생산조건을 검토하고 分離,精製하여 효소특성을 硏究하였다. 효소생산은 배양기간 中 pH 7∼8을 유지시켜 주면서 Baker's yeast 10%와 Ammonium nitrate 0.2%의 첨가로 높은 活性이 있는 효소액을 얻을 수 있었고, 7日間 진탕배양으로 最高에 달하였다. (NH₄)₂SO₄로 분획한 후 Sephadex G-100 column chromatography를 行하여 세 개의 peak를 얻었으며, 첫 번째 peak는 proteose 및 lytic enzyme, 세번째 peak는 lytic enzyme임을 알았다. F.moniliform 효모세포벽 분해효소의 protoplast 제조 최적조건은 pH7.5및 37℃이었고 효모세포벽 분해율은 99.17% 정도였다. Cultural conditions of Fusarium moniliforme for lytic enzyme production have been investigated and the lytic enzyme was purified by (NH₄)₂SO₄fractionation, gel column chromatography and tannic acid precipitation. The lytic enzyme production in the culture of Fusarium moniliforme was greatly affected by pH change of the culture medium. A strong lytic activity on viable yeast cells could be obtained in a medium containing baker's yast (10%) and ammonium nitrate(0.2%) by keeping the pH 7-8 during cultivation. Three protein peaks were obtained from G-100 Chromatography of the crude enzyme. The third peak on chromatogram lysed viable yeast cells and the first had both proteolytic and lytic activity. The optimum pH for producing protoplast of yeast cell was pH 7.5 and the optimum temperature was 37℃.