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김지연 ( Ji Yeon Kim ),장미선 ( Mi Sun Jang ),고바라다 ( Ba Ra Da Koh ),지태경 ( Tea Kyung Ji ),성창민 ( Chang Min Sung ),박다해 ( Da Hae Park ),김현중 ( Hyun Joong Kim ),김은선 ( Eun Sun Kim ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.3
A total of 301 samples of bovine liver, spleen and omasum were collected from butchers and restaurants in Gwangju, Korea during 2012 and all samples were subjected to bacteriological examination and antibiotic residues. Also, this study was performed to survey the consciousness for hygiene of livestock workers who are handling bovine by-products in Gwangju. The detection rate of aerobic plate count (APC) was higher in summer than in other seasons in all by-products (P=0.000). The detection rate of E. coli count was lower in the liver than the spleen and omasum (P=0.000). Twenty four of the samples (8.0%) were contaminated with S. aureus while one spleen sample (0.3%) was contaminated with L. monocytogenes and finally 10 (3.3%) of the liver and omasum samples were contaminated with Cl. perfringens. Five of the twenty-four S. aureus isolates harbored enterotoxin gene. However, the cpe gene of Cl. perfingens was not detected among any of the 10 isolates. Antibiotic residues were not detected in the liver samples. The consciousness survey`s results showed that most of them (58.8%) were safe.
광주지역 소 생고기 선호도 및 유통단계별 세균학적 분석
나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),고바라다 ( Ba Ra Da Koh ),장미선 ( Mi Sun Jang ),성창민 ( Chang Min Sung ),김지연 ( Ji Yeon Kim ),박헌규 ( Heon Gyu Park ),문용운 ( Yong Un Mun ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4
Consumer`s preference and microbial inspections on fresh raw beef were carried out to understand the actual market status in Gwanju, Korea. Over 15 questions on questionnaire by 1,111 randomly selected respondents between April and May in 2011, results showed 65.5% positive on eating fresh raw beef, 63.8% negative on good hygiene condition of fresh raw beef, and 72.5% positive on the secure of the hygiene-safety for priority program, respectively. For microbial inspections, a total of 302 samples were collected from fresh raw beef purchased from slaughterhouse (n=122), transport (n=69) and consumer (n=81) stage, from lettuce (n=30) at consumer stage. The aerobic plate count (APC), E. coli count and food borne bacteria such as Salmonella spp., Listeria monocytogenes, Staphylococ-cus( S.) aureus and E. coli O157:H7 were tested in the samples. As results, the level of count on APC of fresh raw beef ranged 6×10(1)∼1.8×10(5) CFU/g from slaughterhouse, 2×10(2)∼8.3×10(5) CFU/g from transport stage and 1×10(2)∼4×10(5) CFU/g from consumer stage. The level of count on E. coli of fresh raw beef ranged 1∼9×10(1) CFU/g from slaughterhouse, 1∼7×10 CFU/g from transport stage and 1∼5.5×10 CFU/g from consumer stage. In total, 26 S. aureus were isolated, 10 (14.5%) from fresh raw beef at transport stage, 12 (14.8%) from fresh raw beef and 4 (13.3%) from lettuce at consumer stage. Enterotoxin of S. aureus was not detected among 26 isolates. All S. aureus isolates were typed using a DiversiLab(TM) rep-PCR system for genetic similarity test, showing over 95% of genetic relationship amon isolates.