A Study on the Physicochemical Properties of a Sweet Flavor for Oriental Herbal Medicines

Deog-Bin Lim(임덕빈),Ho-Young Choi(최호영),Jae-Seong Rhee(이재성) 대한한의학회 2002 대한한의학회지 Vol.23 No.1

유백피와 자유피의 형태 감별에 관한 연구

김형철 ( Hyung Cheol Kim ),임덕빈 ( Deog Bin Lim ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2

N/A Objective : In order to distinguish morphological characteristics of trunk bark and root bark of Ulmus davidiana var. japonicas (Rehder) Nakai and the trunk bark and root bark of Hemiptelea davidii Planchon were sampled and compared in terms of their external and internal features with flour states according to their medical use, through microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus BHT. Result 1. Internal Features 1) A large parenchymatous cell was observed in the phloem of the slice of both trunk bark and root bark of Ulmi Cortex. However. both of the trunk bark and root bark of Hemipteleae Cortex did not have parenchymatous cell in the phloem: instead, stone cells including much square crystal of calcium oxalate were distributed around fiber bundle, and the parenchymatous cell included much druse crystal of calcium oxalate. 2) In both the Ulmi Cortex and Hemipteleae Cortex, rhytidome was observed in trunk bark, but not in root bark, but in the parenchymatous cell of the root bark of the Ulmi Cortex contained starch grain. 2. Flour States 1) In the flour of root bark of the Ulmi Cortex, a large parenchymatous cell was observed. However, in the flour of trunk bark and root bark of Hemipteleae Cortex, no parenchymatous cel was found; instead, stone cell including square crystal of calcium oxalate and druse crystal of calcium oxalate were observed. 2) There was no remarkable difference between the trunk bark and root bark of Hemipteleae Cortex. However, starch grain was contained in the parenchymatous cell of the root bark of Ulmi Cortex but not in the trunk bark of it. Conclusion There were some morphological differences in external, internal, and flour parts of Ulmi Cortex and Hemipteleae Cortex. In particular, there was a morphological difference in flour states between the trunk bark and root bark of Ulmi Cortex, it is possible to use microscope to distinguish their flour states.

靈仙除痛飮煎湯液의 經時的 效能變化에 關한 硏究

崔永奉(Choi, Young-Bong),林德彬(Lim, Deog-Bin),李暎鍾(Lee, Young-Jong) 대한한의학회 1998 대한한의학회지 Vol.19 No.1

인삼의 형태 감별에 관한 연구

이한구 ( Han Goo Lee ),임덕빈 ( Deog Bin Lim ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2

N/A Objective : To study the morphologic characteristics of Ginseng according to the growing district and stock, Panax ginseng C.A. Meyer growed each in Korea and China, and P. quinquefolium L were collected. The general morphology, and the exterior, interior and the flour of each were comparatively analyzed by microscope. Method : The surface of the transverse section of the specimen was made into a slid by the Paraffin Section method, and then dyed by Safranine Malachite Green method. The flour of the specimen was mounted by a solution of Glycerin, Acetic acid and Water at the rate of 1 : 1 : 1 The samples were observed at the power of 400 by an optic microscope(OLYMPUS-BHT, Japan). Photographs were taken and also the characteristics were sketched in lines. Result The plants used as Ginseng according to the time of year for gathering and growing district were comparative analyzed by optic microscope. The results were as the followings. 1. The external form of Korean Ginseng`s upper portion of root is short but Western Ginseng` is long and has many transverse long fold on it`s main root. In addition Korean Ginseng has a short rhizome and no transverse mark but Mountain Ginseng has the long rhizome and the transverse mark. 2. The internal form, Korean and Mountain Ginseng are similar to each other but Western Ginseng has more developed secondary cork layer than Korean Ginseng. 3. In the flour form, Korean Ginseng and Western Ginseng have many starch particles and one or two comparatively big piece on crystal of calcium hydroxide. But Mountain Ginseng has lesser starch particles and no big piece on it. 4. There were no significant microscopic differences among the flours of Korean Ginseng and Western Ginseng. Conclusion There were some morphological differences in external and internal parts of Panax ginseng C.A. Meyer and P. quinquefolium L. But there was no morphological difference in flour states, therefore it is impossible to use microscope to distinguish each other. On the other hand there was a morphological difference in flour states between Korean Ginseng and Mountain Ginseng, it is possible to use microscope to distinguish their flour states.

동규자와 경마자의 형태 감별에 관한 연구

최정호 ( Jung Ho Choi ),임덕빈 ( Deog Bin Lim ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2

N/A Objective : Malvae Fructus and Abutili Semen come from different plants and medical purpose is different. Even though Abutili Semen(Abutilon theophrasti Medicus) is often used as Malvae Fructus(Malva verticillata Linne). Therefore, In order to distinguish morphological characteristics of Malvae Fructus and Abutili Semen were collected, and their external, internal, and flour states were observed via microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus-BHT. Results : 1. The external form, Malvae Fructus looks like a disk. The surface is bright or dark yellow with a prominent thin pattern, while Abutili Semen is triangular egg shape with dark brown surface and a little fluffy hair on it. 2. The internal form, Malvae Fructus had the following morphological characteristics. Palisade cells are constituted of one row in a shape of long column with very thick cell wall. The brilliant belt is located on the outer fourth of the transverse section. The pigment layer is constituted of cell of one row and filled with yellowish brown or violet matters. Abutili Semen is mostly similar to Malvae Fructus but pigment layer is constituted of cell of 4-5 row. 3. In the flour form, pericarp of Malvae Fructus could be classified square crystals of calcuim oxalate. Some of seed coat epidermis cells of Abutili Semen were cell layer and fibrous layer. These cell layer and fibrous layer have differentiated into unicellular non-glandular hair. Conclusion : There were differences in external, internal, and flour states between Malvae Fructus and Abutili Semen, so that microscope can be used to distinguish between these two.

실리움 응용처방이 高콜레스테롤 흰쥐의 체중 및 혈중 지질 함량에 미치는 영향

任德彬,李暎鍾 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

Psyllium husk, which is the seed of Plantago psyllium L., Plantago arenaria Waldst. and Kit., or plantago ovata Forsk., belongs to Plantaginaceae family. Because it is absortive and contains much mucous components, it is frequently consumed as a mild laxative for obesity, constipation, diarrhea, cystitis and the other symptoms in the western countries. To prove clinical utility, the herbal compounds of psyllium husk(containing psyllium husk, Polygoni Multiflori Radix, Visci Ramulus, and Polygonati Rhizoma) have been dosed to the rats caused obesity by high cholesterol diet. The results are as follows. 1. The herbal compounds of psyllium husk dosed to obese rats are useful for the inhibition of weight gain and the decrease of dietary intake. 2. The herbal compounds of psyllium husk dosed to obese rats show a slight decrease of fecal lipid levels(but without statistical significance). 3. The herbal compounds of psyllium husk dosed to obese rats decrease serum total cholesterol levels. 4. The herbal compounds of psyllium husk dosed to obese rats show a slight decrease of serum triglyceride levels(but without statistical significance). 5. The herbal compounds of psyllium husk show no changes to serum HDL cholesterol levels. These results suggest that the herbal compounds of psyllium husk could be useful for the inhibition of appetite and the decrease of serum cholesterol levels.

連翹敗毒散煎湯液의 經時的 效能變化에 關한 硏究

吉基正,林德彬,李暎鍾 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1

From the ancient times, herbs, animals and minerals were used as natural medicine to cure human disease in diverse form. One of the representive forms in oral intakes is decoction. But medicinal decoction degenerates as time passes by just as all foods do by the environmental factors, such as micro-organism, enzyme, light and the air. Nowadays, as medicinal decoctions are kept in bottles and retort pouch for longtime, some datum point of preservation is necessary. In order to measure the standard statistics for safe preservation, Yeonkyopaedogsan, applyed in case of carbuncle and infection inner boil, is used. According to this experiment which measured pH, specific gravity, anti-inflammatory and antimicrobial effect, the results were as follows : 1. No remarkable change was found in pH and in the specific gravity of the decoction. 2. As for the anti-inflammatory effects induced by carrageenin in mice, no remarkable decrease in its medicinal efficacy was found until the 7th day, but it showed decrease in its medicinal efficacy from the 9th day. 3. As for the anti-inflammatory effects induced by acetic acid induced vascular permeability in mice, no remarkable decrease in its medicinal efficacy was found until the 9th day, but it showed decrease in its medicinal efficacy from the 11th day. 4. As for the antimicrobial effects induced by Staphylococcus aureus ATCC 65389, Shigella sonnei ATCC 9290, Pseudomonas aeruginosa ATCC 27853, Streptococcus pyogenes ATCC 21059, Shigella flexneri ATCC 9199, no remarkable decrease in its medicinal efficacy was found until the 10th day, but it showed decrease in its medicinal efficacy from the 11th day. According to the above results, pharmacological effect is kept until the 9th day, but it showed decrease in its effect from the 11th day. In conclusion, keeping the natural medicinal decoction for more than 9 days should be avoided.

건조된 한약재의 유전자 분석 가능성 연구

崔湖榮,任德彬 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

The isolation of DNA from crude drug has been described by numerous authors, each contributing a different method to overcome the problems that arise when extracting DNA. Fresh of frozen tissue is usually used as a source of DNA for PCR and RAPD analysis. A SDS method for rapid purification of DNA from variety of herbal crude drug, extracting from samples as small as 1㎎, has been optimized. I reached the following conclusion. The yield of DNA was declined after 3 and 4 days drying at 60℃. However, the DNA isolated from all sample at 60℃ in oven was suitable for analysis with random 10-mer primer as the primer. Similar results were obtained with crude drug Angelicae Gigantis Radix from the different drying time. I think that this result can not only be used as a way to discriminate crude drug but it can be also utilized to control the quality of crude drug and the distribution channel in the future.

백두구, 소두구 및 砂仁의 形態 鑑別에 관한 硏究

金鍾元,任德彬,李暎鍾 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Objectives : In order to distinguish Amomi Cardamomi Fructus, Cardamomi Fructus, and Amomi Fructus, their external, internal, and flour states were observed via microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus-BHT. Result : 1. The seed of Amomum cardomomwn Linne is close to a ball-shape, the color of its pericarp is milk-white or light brown with a diameter of 0.8~1.2cm. The seed of Elettaria cardamomum Maton is oval, and the color of its pericarp is light yellow or yellow-green with a length of 1~2cm and diameter of 5~7cm. The seed of Amomum xanthoides Wallich is close to a ball-shape, and the color of its pericarp is yellow-brown or brown, with thin and compact protrusion, and with a length of 1.5~2cm and diameter of 1~1.5cm. 2. As for the internal form, the mesocarp of Amomi Cardamomi Fructus and '1,Cardamomi Fructus shows once-rounded vascular bundles, and that of Amomi Fructus shows once- or twice-rounded vascular bundles. Amomi Cardamomi Fructus has annulus of stone cells layered among vascular bundles, but Cardamomi Fructus and Amomi Fructus do not have them. Endopleura has stone cells, and the stone cells of Cardamomi Fructus are 16~20㎛ long and 10~20㎛ wide, while Cardamomi Fructus itself is 50~60㎛ long and 40㎛ wide, and the stone cells of Amomi Fructus is 12~16㎛ long and 12㎛ wide. 3. In flour states, the cell sizes of endopleura are as follows: Amomi Cardamomi Fructus and Amomi Fructus are small by 9~25㎛, while Cardamomi Fructus is relatively large with a length of 50~60㎛ and width of 40㎛. Conclusion : Significant differences were found among Amomi Cardamomi Fructus, Cardamomi Fructus, and Amomi Fructus in their external forms. In addition, there were also some differences in their internal and flour states. Therefore, they can be distinguished through observation via microscopic examination.

高果糖 誘導 고지혈증 흰쥐에 대한 薏苡仁의 효과

이영종,손영종,임덕빈,이은섭,박중수,김성기 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Objective : In order to examine the effects of Coicis Semen on the hyperlipidemia, the extracts of Job's Tears were given to hyperlipidemic rats induced by fructose-rich chow. Method : The hyperlipidea was induced by fructose-rich chow during five weeks in rats. And the rats were administered with the extracts prepared from husk layer, bran layer, polished rice, unpolished rice or unhulled grain of Job's Tears each others during three weeks. And then, the effects of the extracts on body weight gain, total cholesterol, triglyceride and blood glucose were examined. Results and Conclusion : The extracts prepared from polished rice, unpolished rice or unhulled grain of Job's Tears had no effects on the body weight gains of the corpulent rats induced by a high fructose diet. Meanwhile, the extracts prepared from the unhulled grain (Coicis Semen), unpolished rice, and bran layer of Job's Tears decreased the levels of blood total cholesterol. and triglyceride, each others. And the effect of unhulled grain was more stronger than those of the others. The results suggested that unhulled grain had some materials useful for alleviating the hyperlipidemia, and the effective molecule is existed in the surface of unhulled grain.