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Pseudomonas syringas 빙핵활성 단백질 유전자의 크로닝
한문희,홍주봉,이혜주,우진경,김정하,함경수 생화학분자생물학회 1993 BMB Reports Vol.17 No.3
A gene codes for a protein with ice-nucleation activity has been cloned from a plant pathogenic strain of Pseudomonas syringae in Korea. Once the gene has been introduced into E. coli, the transformed E. coli showed strong ice-nucleating activities; while the freezing temperatures of the 10 mM phosphate buffer, pH 7.0, with E. coli were from -10℃ to -14℃, E. coli once transformed with pGINP-1 raised the freezing temperature to about -5℃. Restriction enzyme mapping of pGINP-1 showed two Pvu II and two Sal I sites in 4.7 kbp DNA fragment flanked with Bgl II and EcoRI restriction enzyme sites.