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      • 한국, 일본 및 중국산 깜부기병균의 분포, 기주 및 발생 현황

        이왕휴,이귀재,이용훈 한국식물병리학회 2002 식물병연구 Vol.8 No.1

        Different types of smut fungi have been reported from different geographical places, depending on the distribution of host plant. Therefore, a survey was conducted to investigate the host plant of smut fungi and occurrence situations in three Asian countries. As a result, 12 genera of 4 families and 19 species, 24 families 87 genus and 191 species, and 12 families 140 species were occurred in Korea, Japan and China, respectively. Six genera(Entyloma, Tilletia, Sorosporium, Sphacelotheca, Urocystis, Ustilago) of Korean smut fungi species were reported until 1998. In Japan, Ito reported for first time 14 genera and 140 species of smut fungi in 1936, and then were more described into 17 genera 114 species by Kakishima in 1984. Two genera(Schizonella and Nannfeldtiomyces) and 8 species were newly listed, later by other researchers. In China, Ling reported that smut fungi classified into 14 genera, 109 speciesin in 1953. In addition, Liroa, Glomosporium, Gahmphopora and Microbotryum genus including 49 species were recently reported, thus 18 genera and 158 families of smut fungi in China. These results suggest that the number of plant related with smut fungi is seemed to be more increase in future. Smut disease occurred on the whole or part of plant and flower.

      • 생강근경부패병균에 대한 특수이온의 영향 및 전기영동 양상

        이왕휴,심형권,이두구 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1

        Ginger(Zingiber officinale L.), a popular beverage crop, is one of the many distinctive products in Chonbuk province. Al and Fe ion content in several areas (Eunhari, Eouri, and Yulsori) were measured to investigate the incidence rate of rhizome rot. Al ion content of soil in ginger cultivated field was related with rhizome rot. Eunhari, 2,000-3,465ppm of Al ion in soil showed a lower rate of disease incidence. Al ion content of this area increased until august, but was rapidly decreased at the harvest season. Fe ion content of Yulsori field, high incidence rate of rhizome rot, contained greatly higher than those other fields. Another experiment was conducted to investigate supplemental Al+3 or Fe+3 In the medium, When 100ppm of Al+3 or Fe+3 was supplemented to 1/10 tryptic soy medium, Fusarium oxysporum f.sp. zingiberi was suppressed 6% compared to control. Pythium zingiberum was almost suppressed by supplemental 25ppm of Al+3 or 100ppm of Fe+3 in the medium. Electrophoresis was used for defection of these pathogens as follows. Infected ginger plant to Fusarium or Pythium showed different protein band at Rf 0.26 and 0.76, respectively. P. zingiberum also showed protein band at Rf 0.02, 0.16, 0.38, 0,45 and 0.60. Both peroxidase band and esterase band of P. zingiberum resulted in Rf 0.6,0.64, 0.67 and 0.13,0.21, 0.65, and 0.70, respectively.

      • 水稻 育苗의 立枯病菌에 대한 拮抗細菌의 選拔 및 效果

        李王休,李斗求,朴建鎬 전북대학교 농업과학기술연구소 1989 農大論文集 Vol.20 No.-

        The antibiotic bacteria damping-off pathogens (Fusarium,Pythium graminicola etc.) of rice seedling were isolated from rice (Oryza sativa L) rhizosphere and phyllosphere of tested varieties. The antibiosis between pathogens and isolated bacteria were tested on a medium (potato dextrose agar). and rice seeds treated the antagonistic bacteria were sown in rolled paper towel and in nursery flat. The results are summarized as follows; 1. The rate of damping-off of non-seed disinfection was significantly different among the tested rice varieties. 2. The 480 bacterial isolated were isolated from rice rhizosphere and phylloephere. eighty isolated among them were tested for antibiotics. Most of them were antibiotic bacteria to Rhizoctonia solani, but only a few of them were to Pytium sp. and Fusarium. sp·.. 3. The 39% of pathogens isolated from seedling shown damping-off of tested varieties were Fusarium spp. But the other pathogens of damping-off were not identified. 4. In rolled paper towel test, the results of seed bacterization with the antibiotic bacteria showed that the bacteria induced increasing root length of seedling. Correlation between shoot length and root length of rice seedling treated bacterization was showed positive correlation coefficient (r=O.6668***). 5. The effect of seed bacterization in rice showed a little promoting seed germination and preventing damping-off in nursery flat.

      • SCIEKCI등재
      • 交配型 및 酵素패턴에 따른 감자 疫病菌의 分類

        李王休 전북대학교 농업과학기술연구소 1992 農大論文集 Vol.23 No.1

        Twenty isolates of Phytophthora infestans from the foliage of blighted potatoes growing in the Chonbuk province, Korea were analyzed in 1991. All isolates were categorized as the At mating types by compatibility determination. There were significant differences in number of zoospores of plates among the fungal strairis. Mycellium growth on oat meal agar was more abundant than on 10% of V-8 juice cleared agar medium. The electrophoretic patterns at two enzyme loci, glucose Phosphate isomerase(GPI), and peptidase(PEP) resulted in mycellia from the isolates as A2: types. GPI had no less than two loci and two electromorphs at 100/100 and 157/157, whereas PEP had also two loci and two electromorphs at 93/93 and 59/59.

      • KCI등재SCOPUS

        생강 근경부패병의 발생 생태 및 토성 , 토양화학성 및 토양 생물성과의 관계

        이왕휴,이두구 ( Wang Hyu Lee,Doo Ku Lee ) 한국환경농학회 1998 한국환경농학회지 Vol.17 No.1

        The ecology of rhizome rot incidence rates between Seosan, Chungnam and Bongdong, Chonbuk province were surveyed in this experiment. The rhizome rot incidence rate of Seosan was lower than that of Bongdong. The average ginger product of former province was 1,140㎏, whereas it`s 818㎏ per 10a. in latter province. Treatment after disease incidence frequently used fungicide at Seosan. There were different soil physiological property between fields. Although Eunhari and Yulsori were belong to Bongdong province, incidence rate was low at Eunhari, contained 23% clay and high incidence rate at Yulsori, approximately contained 60% silt. Many soil chemical properties of cultivated area were belong to optimum concentration range, but phosphate(P) was higher than optimum concentration, 450-550ppm. The number of soil fungi in the middle of cultivated season tended to show the highest. Both bacteria and actinomyces` number was similar to the result of fungi. The bacteria/fungi ratio was greatly different between surveyed fields.

      • KCI등재

        감자 역병균에 대한 스테롤류의 영향 및 감자절편에서의 난포자 형성

        이왕휴,이용훈,이두구 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.1

        역병균 (Phytophthora infestans)의 균사 신장 및 난포자 형성에 미치는 생리 화학적 영향을 알아보고자 국내에서 분리된 $A^2$형인 KM10, U6, MHB6, CDB6, JD1과 일본에서 분양 받은 $A^1$형인 F8l7, DNC303, $A^2$ 형인 IB908, DN107 균주를 이용하여 공시 배지별 및 V-8A 배지에 cholesterol, $\beta$-sitosterol및 lecithin을 첨가했을 때의 영향을 조사하고, 식물체상에서의 난포자 형성여부를 조사한 결과, 다음과 같은 결과를 얻을 수 있었다. 1. 균사 신장은 V-8A, RMA에서 양호하였고, 난포자 형성은 V-8A에서 가장 많았다. V-8A에 cholesterol, $\beta$-sitosterol 및 lecithin을 첨가하였을 때 접종 10일 후 균사 신장은 무처리 대비 각각 16.6, 8.3, 5.2% 더 신장하였으며, 난포자 형성수는 각각 76.0, 58.0, 34.6% 크게 증가하였다. 2. $A^1$형과 $A^2$형이 동시에 존재할 경우 식물체상에서 난포자를 형성하는지 알아보고자 식물체 조직편에 $A^1$, $A^2$형을 대치 배양하여 난포자 형성을 유도한 결과, 난포자의 형성률 및 수는 적었으나, 식물체 상에서도 난포자 형성이 가능한 것을 확인 할 수 있었는데, 금후 이에 대한 세심한 주의가 필요할 것으로 생각된다. The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.

      • 사탕무우 種子의 박테리제이션에 관한 硏究 : IV.근면의 세균정착 및 세균의 동정 IV.Bacterial colonization of rhizoplane and identification of antibiotic bacteria to damping-off pathogen of suga

        李王休 전북대학교 농업과학기술연구소 1991 農大論文集 Vol.22 No.1

        Scanning-microscopic examination resulted that seed-treated bacteria became colonized with the rhizoplane of the sugar beets(Beta vulgaris L. var saccharifera) .The colonization showed remarkable differences among the bacterial strains. By testing bacteriological characteristics 10 bacterial strains were further selected from the 45 strains (Ann.Phytopath. Soc. Japan 52 175) of the net regions tested. Eight bacterial strains of them were identified as fluorescent pseudomonads and the rest were Bacillus sp. and Enterobacteriaceae respectively.

      • SCIEKCI등재
      • SCIEKCI등재

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