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- 저자 : 이승길 ( Sok Woo Lee (검색결과 5 건)
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배양포유동물세포에서 환경돌연변이원에 의해 유발된 자매염색분체교환의 분석에 관한 연구
이석우,남정구,남재환,이병광,김은영,이승길 ( Sok Woo Lee,Joung Koo Nam,Jae Hwan Nam,Byoung Kwang Lee,Eun Young Kim,Seung Gil Lee ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.2
The assay of SCEs has been used to measure mutagenicity induced several mutagens to high vertebrates. In this study, we used BHK-21, CHO-K1 and human peripheral lymphocyte as culture cell, and calcium chloride dihydrate(CaCl_2·2H_2O), sodium hypochloride(NaOCl), phenol(C_6H_5OH), benzo(a)pyrene(C_20H_12), carbon tetrachloride(CCl_4) and chloroform(CHCl_3) as mutagens. We examined and compared frequency between spontaneous and induced SCEs by mutagens. The mean spontaneous SCEs of human lymphocyte from 4 donors is scored 6.92±1.54(SCEs/cell) and BHK-21 is scored 0.18±0.03(SCEs/chromosome) and CHO-K1 is scored 0.61±0.08(SCEs/chromosome). In order to decided concentration of mutagens which is enough to induce SCEs and to effect SCEs score, we observed cell cytotoxicity and cell metaphase dependent on several concentration of mutagens. And then, using that concentration as control, we examined SCEs frequency induced three different concentration of mutagen. The increase of SCEs frequency induced by calcium chloride dihydrate is very little, but benzo(a)pyrene and phenol are great increase. Especially, we cannot find metaphase over 0.006mM benzo(a)pyrene and 1mM phenol. Although the increase of SCEs frequency induced by CHCl_3 is little, we observed many acentromeric chromosome in cell culture over 25mM CHCl_3. And we detected that SCEs frequency related with chromosome length of test cell.
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LHR 조건하에서 배양 인체 백혈구가 나타내는 SCE 와 excision DNA repair 능력
이광호,이석우,이승길,지성길,정계준,원태웅,박현숙 한국유전학회 1987 Genes & Genomics Vol.9 No.3
Go human lymphocytes were treated prior to culture initiation with 7 cytostatics (MMC, CLM, MTX, Ara-C, Act-D, HU, 5-FU) in order to determine whether cytostatics having different mode of action on DNA are equally effective in inducing DNA lesions in Go human lymphocytes. Go lymphocytes damaged with cytostatics showing positive results (MMC, CLM) were allowed to repair DNA damages under LHR conditions for 12-36 hr to compare excision DNA repair capacity in exposed lymphocytes. Additionally, we carried out a series of experiments to investigate the relevance of Go exposure duration (1-4 hr), SCE induction and excision repair capacity during limited LHR period (12 hr), the effect of BUdR on excision DNA repair activity in Go lymphocytes under LHR conditions and the characteristics of the unexcision repaired lesions remained in the DNA of Go lymphocytes after LHR that can lead to SCE formation, MMC and CLM induced exposure duration-related increase in SCEs, while MTX, Ara-C, Act-D, HU and 5-FU showed only minor variations in SCE indutions during Go exposure of 1-4 hr. There was a significant difference between SCEs after 2 hr of MMC/CLM treatment. and those after 12-36 hr LHR conditions. The effect of limited LHR period (12 hr) on SCE value was not dependent on the exposure duration or induced SCE frequencies. In cultures exposed to CLM, BUdR induced LHR period-dependent increase of SCEs during LHR period. LHR-allowed lymphocytes showed more rapid decrease of SCE as compare to those of cultures without LHR conditions. MMC-induced SCEs were more slowly decreased than CLM-induced SCEs with or without LHR conditions.
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사람의 염색체에 제한효소 처리후 나타난 band pattern 과 그 작용 기작
이석우,이승길,남재환,원태웅 한국유전학회 1994 Genes & Genomics Vol.16 No.3
In situ digestion experiment using restriction enzyme applied to fixed human chromosome brought the following results ; First, Asn I and Dra I that recognized AT-rich sequence produced C-like band patterns and telomere bands which are the distintive feature of R-like band patterns. Therefore the existence of recognition base sequence greatly influenced for the action of restriction enzyme on fixed chromosome. Second, DNA loss had decisive influence upon C-like band pattern produced by restriction enzyme. But chromatin comformational change seems to have acted upon G-like band pattern. Third, analysis of the result of electrophoresis showed that among DNA fragments separated from a chromosome, by restriction enzyme, some DNA fragments that had large size and were not extracted under the influence of non-histone protein were remained on fixed chromosome. Fourth, human constitutive heterochromatin turned out to be heterogeneous by satellite DNA of highly repeated sequence. In conclusion, there's no doubt that DNA loss plays the most important role in the formation of bands by restriction enzyme and that DNA loss depends on the frequency of recognition sequence of restriction enzyme.
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Herpes Simplex Virus 가 배양된 백혈구의 염색체에 미치는 영향에 관한 연구
이석우,이승길 한국유전학회 1986 Genes & Genomics Vol.8 No.1
Various types of structural aberrations of chromosome such as chromosome breaks, chromatid breaks, chromatid interchanges, dicentrics and chromosome pulverizations were observed in cultured normal human leucocytes infected with herpes simplex virus type 1, 2 and their mixture. In all cases tested, the chromosome type breaks appeared to be dominant. The chromosome aberrations increased in proportion to the dosage of virus inoculated into the cultures. Especially, such findings were remarkable in HSV-1 treated groups. The distribution of breaks depending on the chromosome groups and arms appeared to be highly correlated to the relative length of chromosome except chromosome No.3. Higher frequencies of breaks on the chromosome No.3 were supposed to be due to the its sensitivity to HSV. Also it was supposed that HSV-1 and 2 induce the aberrations on the arms and groups of chromosome by almost the same ways.
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Kim, Eun Young,Lee, Sok Woo,Lee, Seung Gil 한국유전학회 1992 Genes & Genomics Vol.14 No.4
Chinese hamster cell deficient in hypoxanthine-guanine phosphoribosyl transferase(HPRT) was fused with normal human female lymphocyte. Chinese hamster-human cell hybrid clones retaining human inactive X chromosome were selected. The hybrid cells were treated with the de(hypo)methylating agent 5-azacytidine(5-AzaC) and tested for derepression of the three inactive human X-linked genes, Hprt, glucose-6-phosphate dehydrogenase(G6pd), and phosphoglycerate kinase (Pgk). Derepression of Hprt gene was detected by pulse-labeling and histochemical methods. The frequency of derepression of Hprt gene after the treatment with 5-AzaC for one cell cycle was 21-fold greater than that observed in the untreated hybrid cells. Data using electrophoretic method showed that the hybrid cells in which Hprt was derepressed also expressed human G6PD and PGK, but that the frequencies of derepression of Pgk and G6pd genes were independent. These results support that the derepression of the inactive human X-linked genes is affected by 5-AzaC-induced de(hypo)methylation of DNA.
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