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      • KCI등재

        감국(Chrysanthemum indicum L.) 추출물과 분획물의 항산화 및 α-Glucosidase와 PTP1B 저해 활성

        황성우,이훈연,차지윤,김보람,박민,이보리,문광현,이정호,김대근,김욱,정경옥,이영미,Hwang, Sung-Woo,Cha, Ji-Yun,Lee, Hoon-Yeon,Kim, Bo-Ram,Park, Min,Lee, Bo-Ri,Moon, Kwang- Hyun,Lee, Jeong-Ho,Kim, Dae-Geun,Kim, Wook,Jeong, Kyoung-Ok,Lee, 동아시아식생활학회 2017 동아시아식생활학회지 Vol.27 No.2

        본 연구에서는 국화과에 속하는 Chrysanthemum indicum L.(CI)의 70% EtOH 추출물 및 분획물(dichloromethane, ethyl acetate 및 H2O)의 폴리페놀 함량, 플라보노이드 함량, 항산화 활성 및 생리활성을 평가하였다. 항산화 활성으로는 DPPH, ABTS 및 superoxide 소거 활성을 확인하였고, 생리활성 평가는 ${\alpha}$-glucosidase와 PTP1B 저해 활성을 측정하였다. 그 결과, CI 70% EtOH 추출물에서 폴리페놀과 플라보노이드의 함량은 각각 30.20 mg 및 31.58 mg인 것으로 확인되었다. 여러 분획물 중 EA 분획물이 폴리페놀(87.87 mg)과 플라보노이드(65.12 mg)의 함량이 가장 높았다. EA 분획물의 DPPH, ABTS 및 SOD 소거능은 각각 93.84%, 93.38% 및 84.68%의 억제를 나타냈다. EA 분획물은 70% EtOH 추출물 및 다른 분획과 비교하여 가장 높은 항산화 활성을 보였다. 또한 EA 분획물($20{\mu}g/mL$)의 ${\alpha}$-glucosidase 및 PTP1B 억제 결과는 다른 시료에 비해 각각 30.29% 및 99.24%의 가장 높은 억제를 나타내었다. 본 연구 결과를 통해 감국의 EA 분획물이 70% EtOH 추출물 및 다른 분획물보다 많은 항산화 및 항당뇨 성분을 함유하는 것으로 보여진다. 결론적으로 우리는 CI의 천연 항산화 물질 및 기능성 물질에 성능이 좋아 화장품 첨가제나 음료로도 개발이 가능할 수 있을 것으로 사료된다. In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of ${\alpha}$-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction ($20{\mu}g/mL$) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.

      • 기업의 사회적 책임에 대한 소비자 관점에서의 탐색적 연구

        노예슬(Noh Ye seul),이보리(Lee Bo ri),황혜선(Hwang Hye sun) 충북대학교 생활과학연구소 2015 생활과학연구논총 Vol.19 No.2

        This study examined consumers' perception on corporate social responsibility (CSR). As the market is becoming more competitive and consumers awareness of social problems is rising, voices demanding CSR get the attention of many companies. This study attempted to analyze how consumers perceive the concept and the range of CSR. Consumer demand for CSR activities and their response to companies' CSR activities were analyzed. CSR activities were classified into 4 dimensions: company's internal management, consumer-related, the government-related and society-related CSR activities. The major findings of this study are as follows. First, consumer-related activities were perceived as the most appropriate and needed CSR activities, while the perception of society-related activities was lowest. Second, respondents who have higher sense of responsibility as consumers have more positive attitude to CSR activities and have more intention to purchase a product/service that is related to the companies' CSR activities. Third, respondents' subjective norm toward CSR has an effect on the response to CSR activities.

      • KCI등재

        감국(Chrysanthemum indicum L.) 추출물과 분획물의 항산화 및 α-Glucosidase와 PTP1B 저해 활성

        황성우(Sung-Woo Hwang),이훈연(Hoon-Yeon Lee),차지윤(Ji-Yun Cha),김보람(Bo-Ram Kim),박민(Min Park),이보리(Bo-Ri Lee),문광현(Kwang- Hyun Moon),이정호(Jeong-Ho Lee),김대근(Dae-Geun Kim),김욱(Wook Kim),정경옥(Kyoung-Ok Jeong),이영미(Young 동아시아식생활학회 2017 동아시아식생활학회지 Vol.27 No.2

        In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of α-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction (20 μg/mL) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.

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