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RISS 인기검색어
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- 저자 : 이민혁(Min-Huck Lee) (검색결과 3 건)
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Salix gracilistyla 추출물의 미백, 항염증 및 항노화에 미치는 영향
박지혜 ( Ji-hye Park ),이민혁 ( Min-huck Lee ),강상모 ( Sang-mo Kang ) 한국미용학회 2019 한국미용학회지 Vol.25 No.3
This study was carried out to examine whitening, anti-inflammatory and anti-aging effects of pussy willow (Salix gracilistyla). S. gracilistyla was extracted with 60℃ and 100℃ water, 60% and 95% ethanol. As a result of investigate the usefulness of S. gracilistyla extract as a cosmetic substance, S. gracilistyla extract showed cytotoxicity, anti-inflammatory effect, whitening and anti-aging effect. The ethanol extract of RAW 264.7 cell showed 50 μg/mL (p<.05), which was significantly inhibited by NO production. B16F10 cells showed a tendency to decrease in proportion to the concentration in all extraction methods. Among them, the 95% ethanol extract showed a tendency to decrease the melanin content significantly at 10 μg/mL compared to other extraction methods (p<.001). The anti-aging effect was confirmed by significantly inhibiting the expression of MMP-1 in HDF cells at 25-100 μg/mL (p<.01). In the tyrosinase inhibitory assay, the diphenolase activity was decreased in proportion to the concentration in all extraction methods, and the whitening effect of all S. gracilistyla extracts was significant at 0.5 mg/mL (p<.001). Therefore, 95% ethanol extract of ethanol and hot water extracts showed the best anti-inflammatory, whitening, and anti-aging effect.
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Salix gracilistyla 추출물을 함유한 화장품의 항산화 및 피부개선 효과
박지혜 ( Ji-hye Park ),이민혁 ( Min-huck Lee ),강상모 ( Sang-mo Kang ) 한국미용학회 2019 한국미용학회지 Vol.25 No.3
This experiment was conducted in order to understand the usability of Salix gracilistyla as cosmetics. For this purpose, S. gracilistyla extract was extracted with 60% ethanol at 60 ℃ for 3 hours, 95% ethanol at room temperature for 24 hours, distilled water at 100 ℃ for 3 hours and distilled water at 60 ℃ for 6 hours. Each antioxidative effect of extract was evaluated. As a result, S. gracilistyla extract contained a large amount of polyphenols and flavonoids. In addition, DPPH radical scavenging activity also showed a high concentration-dependent antioxidant activity. Among them, the best results were obtained when the extract was extracted with 95% ethanol at room temperature for 24 hours. To address a clinical trial was conducted for eight weeks targeting the total number of nineteen middle-aged women. And the detailed changes were observed variables concerning skin surface. As a result, in experimental group, there was statistical significance on facial skin: increase in moisture contents (p<.01), increase in sebum contents (p<.05), decrease in erythema index (p<.05), decrease in melanin index (p<.01), and improvement in skin tone (p<.05). In addition, And there was a statistically significant on neck skin: increase in moisture contents (p<.01), an increase in sebum contents (p<.05), a decrease in melanin index (p<.001), and an improvement in skin tone (p<.01), Thus, S. gracilistyla extract showed anti-inflammatory and anti-melanin activity, and clinical trials showed significant changes, increase in moisture and sebum contents, decrease melanin and erythema index, which proved highly likely to be developed as a functional cosmetics materials.
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