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적혈구 막에 관한 연구(I) Palmitoyl Carnitine에 의한 소 적혈구 막의 용해
이강순,장정순,조기승,이강석,Rhee, Kang-Soon,Chang, Chung-Soon,Cho, Key-Seung,Lee, Kang-Suk 생화학분자생물학회 1975 한국생화학회지 Vol.8 No.1
소의 적혈구막을 palmitoyl carnitine으로 처리하였을 때 단백질, 인지질 및 cholesterol이 동시에 완전히 용해되었고 palmitoyl carnitine에의 한 지질의 유리 및 micelle의 형성이 없는 것으로 보아 적혈구막은 lipoprotein 형태로 용해됨을 알았다. 소 적혈구막의 용해는 palmitoyl carnitine이 막의 lipoprotein과의 친수성 결합과 소수성 결합을 하므로써 용해되며 용해도는 소수성 결합 정도에의 존한다고 본다. Palmitoyl carnitine에의 한 혈구막 용해에 있어서 ATPase($Na^+$, $K^+$ dependent)와 glucose-6-phosphatase의 활성도는 저해 받지 않았으며 오히려 palmitoyl carnitine의 농도에 따라 각각 28-43%, 56-110%가 증가하였다. 용해된 막단백질에 있어 pH에 따른 용해도의 상태는 pH 5.0에서 92%의 단백질이 침전되었다. 용해된 막단백질을 Tris-glycine buffer, pH 8.3로 polyacrylamide gel electrophoresis를 행하였을 때 분자량이 450,000-70,000인 8개의 band로 분리되었다. The mechanism of solubilization of the ox-erythrocyte membrane was investigated with palmitoyl carnitine. When the membrane was treated with palmitoyl carnitine, the membrane protein, phospholipids and cholesterol were completely solubilized. It was confirmed that lipids were not liberated from membrane lipoprotein and not form micelles with palmitoyl carnitine. As a consequence, membrane was solubilized as a form of lipoprotein. In the present study, the results indicated that the erythrocyte membrane was solubilized by involvement of ionic and hydrophobic interaction of membrane lipoprotein with palmitoyl carnitine. The solubility of erythrocyte membrane depended upon the degree of the hydrophobic binding with palmitoyl carnitine. In the complete solubilized membrane, the activities of ATPase($Na^+$, $K^+$-dependent) and glucose-6-phosphatase were not affected or rather slightly increased of 28% and 56% as compared with intact membrane, respectively. The effect of pH on solubilized membrane protein showed that 92% of protein was precipitated at pH 5.0. In polyacrylamide gel electrophoresis with Tris-glycine buffer, pH 8.3, solubilized membrane protein was separated into 8 bands with molecular weight ranged from 450, 000 to 70, 000.
적혈구 막에 관한 연구 ( 1 ) Palmityoyl Carnitine 에 의한 소 적혈구 막의 용해
이강순,장정순,조기승,이강석 ( Kang Soon Rhee,Chung Soon Chang,Key Seung Cho,Kang Suk Lee ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.1
The mechanism of solubilization of the ox-erythrocyte membrane was investigated with palmitoyl carnitine. When the membrane was treated with palmitoyl carnitine, the membrane protein, phospholipids and cholesterol were completely solubilized. It was confirmed that lipids were not liberated from membrane lipoprotein and not form micelles with palmitoyl carnitine. As a consequence, membrane was solubilized as a form of lipoprotein. In the present study, the results indicated that the erythrocyte membrane was solubilized by involvement of ionic and hydrophobic interaction of membrane lipoprotein with palmitoyl carnitine. The solubility of erythrocyte membrane depended upon the degree of the hydrophobic binding with palmitoyl carnitine. In the complete solubilized membrane, the activities of ATPase (Na^+, K^+-dependent) and glucose-6-phosphatase were not affected or rather slightly increased of 28% and 56% as compared with intact membrane, respectively. The effect of pH on solubilized membrane protein showed that 92% of protein was precipitated at pH 5.0. In polyacrylamide gel electrophoresis with Tris-glycine buffer, pH 8.3, solubilized membrane protein was separated into 8 bands with molecular weight ranged from 450,000 to 70,000.
申榮佑,李康淳 순천향대학교 1987 논문집 Vol.10 No.1
As a serial experiments with fifteen kinds of antibiotics, amoxycillin, ampicillin, cepharolidin, hetacillin, penicillin, dicloxacillin, cephalothin, cloxacillin, erythromycin, cephalexin, tetracycline, choloramphenicol, gentamicin, kanamycin and neomycin, were investigated for radition effects on the antibiotics after 60Co irradiatied with various dose in aqueous and dried states in media. The results were as follows: 1) In aqueous states, amoxycilin, ampicillin, cepharolidin, and hetacillin were minimum inhibitory concentration 90 at radiation doses of 50 Krad, and penicillin, dicloxacillin, cephalothin, cloxacillin, erythromycin and cephalexin were at radiation does of 500 Krad, however, the tetracyclin, chloramphenicol, gentamicin and kanamycin were at radition doses of 2.5 Mrad, respectively. 2) In dry states, all antibiotics were stable at radiation does of 5 Mard. 3) On the other hand, neomycin in aqueus state was rather increased 118% and 800% of the antibiotic activity when the E. coli ATCC 113-3 and B. subtilis ATCC 6633 were used as a standard microorganisms, respectively. 4) In physico-chemical properities and biological activities, of the tetracycline HCI in dried state remained radio-resistant up to exposure of 10 Mrad, from the results of this study, author suggest that radiosterilizationb of antibiotics is more recommendable in the dried state than in aqueous solution.
안무영,고광균,이강순 순천향대학교 1992 논문집 Vol.15 No.2
A new plating medium from the extract of Solen gouldi has been developed as alternative to MacConkey agar for the isolation of Shigella. The new medium was tested for the growth of Shigella, Salmonella E. coli, Proteus, Vibrio, Pseudomonas, Staphylococcus and their ability form colonies, and that was compared with MacConkey and Brain Heart Infusion agar media. The following results were obtained. 1. Shigella dysenteriae and Escherichia coli grow best as large colonies on Solen agar medium. 2. Salmonella and Proteus grow well on Solen agar and Brain Heart Infusion agar but MacConkey agar was inhibited. 3. Vibrio parahaemolyticus and Vibric vulnificus was inhibited on Solen agar than thiosulfate-citrate-bilesalt-sucrose agar. 4. The growth of Staphylococcus aureus was highly inhibited on Solen agar media.
전리방사선이 Salmonella 균체 성분에 미치는 영향
이강순,박노춘 순천향대학교 1991 논문집 Vol.14 No.2
IN order to investigate the effect of gamma radiation on Salmonella typhi, Ty2, the components of amino acids, proteins, carbohydrates and lipids in irradiated cells were compared with those in unirradiated control cells respectively. The results obtained were as follows : 1. The inactivation curves of Salmonella typhi with Co? r-ray irradiation were exponential over a wide range to the irradiated doses, 2. Dose for the inactivation factor of 10? was 94.0 Krad in physiological saline or in phosphate buffered saline, 104.2 Krad in nutrient broth, 220.4 Krad in frozen state, 552.0 Krad in dried state, 88.3 Krad in the abundance of oxygen and 188.0 Krad in the deficience of oxygen, respectively. 3. Five consecutive irradiation of Salmonella typhi suspension at the does of 90 Krad gave no additional increase in resistance. 4. Even at the smallest does of 500 Krad, compositions of amono acids, proteins, carbohydrates, and lipids were more or less decreased and the distinct banding patterns were also lost decreased and the distinct banding patterns were also lost possibly due to degradation of the protein molecules.