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Effects of Oxolinic Acid on Microbial Community under Simulated Marine Fish Farm Environment
윤덕현,김무찬,Yoon Duk-Hyun,Kim Mu-Chan The Korean Society of Marine Environment and safet 2006 海洋環境安全學會誌 Vol.12 No.2
The microbial response after treatment of antibiotics was studied for investigating the resistance pattern under simulated aquaculture environment. A marine microcosm was developed for marine fish farm environment using artificial seawater and sediment. Oxolinic acid, which has been commonly used in aquaculture, was employed for the experiment. Resistance patterns and the changes of microbial community were monitored before. during and after use of oxolinic acid. Vibrionaceae was the dominant bacterial species throughout the experiment, consisting 65-75% of total bacterial number in fish farm environment. However, some gram-positive bacteria, Micrococcos sp. and Bacillus sp. strains in marine farm environment were increased in proportion to their number during the treatment. ETS activity of the bacterial communities in aquaculture environment was reduced to 42-67% during the treatment of oxolinic acid. But recovering trends of bacterial number were also detected immediately after cease of oxolinic acid treatment. Frequent treatment of oxolinic acid under the simulated fish farm environment showed bacterial resistance to increase sharply. 이 연구는 해산어 양식장 환경을 재현한 해양 microcosm을 이용하여, 양식장에서 빈번히 사용하고 있는 옥소린산에 대한 미생물이 나타내는 항생제 내성획득에 관해 알아보고자 하였다. 옥소린산 처리 전과 후의 세균상을 비교한 결과, 비브리오 과 세균은 실험기간 전반에 걸쳐 65-75% 정도로 우점하였으며, 그람양성세균인 Micrococcos sp. 와 Bacillus sp. 는 옥소린산 처리 기간 중에 출현 빈도가 증가하였다. 해산어양식 환경에서 세균의 ETS 활성은옥소린산 처리 기간중 42-67%로 줄어들었지만, 옥소린산의 처리가 종료된 후에 세균은 다시 회복되었다. 해산어 양식장에서 옥소린산의 빈번한 사용은 옥소린산에 대한 세균의 내성을 증가시키는 것으로 관찰되었다.
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윤덕현,김무찬,Yoon Duk-Hyun,Kim Mu-Chan The Korean Society of Marine Environment and safet 2006 海洋環境安全學會誌 Vol.12 No.2
해양환경에서 오염물질의 거동은 화학적 특성뿐 아니라 부유물질 표면의 정전하, pH 및 염분과 같은 환경 인자에 크게 영향을 받을 것이다. 이 연구는 해산어 양식장에서 빈번히 사용하고 있는 항생제인 옥소린산의 거동이 여러 종류의 부유물질, 염분 및 pH에 따라 어떻게 변동하는가를 HPLC에 의한 화학적 분석과 bioassay 분석으로 검토하였다. 부유물질의 농도가 증가할수록 옥소린산은 HPLC 분석에 의해서도 저농도로 검출되었고, 생물활성에 의한 bioassay 분석으로도 현저히 감소하는 것으로 나타났다. 이 결과는 부유물질이 옥소린산을 흡착하여 수서환경에서 제거하는 역할을 한다는 것을 알 수 있다. 그리고 옥소린산은 염분 40%o과 pH 7에서 bioassay 분석에 의해 생물활성이 약간 저해되지만 HPLC 분석은 조금 다른 양상을 보였다. The fate of chemical pollutants in the aquatic environment is generally considered to be strongly influenced by environmental factors such as pH, salinity and electrostatic charges on the surface of particles ai well as by the characteristic of chemicals. Oxolinic acid was measured by chemical analysis using HPLC to determine the effect of salinity, pH and suspended solids on chemical binding and by bioassay for measuring bioactivity. The higher contentration of suspended solids in the medium, the lower concentration of oxolinic and was detected in measurements from by both HPLC and biosssay analysis. This indicates particle may have a stronger binding or absorption effect on oxolinic acid. Bioassay analysis showed weaker bioacivity at higher salinity and pH 7.0, but this result of bioassay analysis was different from the result of HPLC.
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수산물 Eco-label CoC에 대한 식품안전 접근방안 연구: AHP 기법을 통한 요구사항 분석을 중심으로
서종석 ( Jong Seok Seo ),서영환 ( Young Hwan Seo ),윤덕현 ( Duk Hyun Yoon ),서원철 ( Won Chul Seo ),옥영석 ( Young Seok Ock ) 한국수산경영학회 2015 수산경영론집 Vol.46 No.3
The purpose of this study is to find food safety approach in the Eco-label Chain of Custody(CoC) which is only focused to traceability. Because, consumers want to be assured the certified seafood comes from sustainable fishery as well as hygienic. In order to this approach, we used Analytic Hierarchy Process(AHP) method as belows. We first understood the CoC criteria for using pair-wise comparison and analyzed and selected each Eco-label certifications and standards. Second, we carried out a survey to the targeted standard Marine Stewardship Council(MSC) CoC auditors all over the world and analyzed the priorities of food safety approach to 4 principles and 12 criteria belong the MSC CoC Standard. As the results, we found out that‘Management System’has the highest priority in the principles and ‘.Documentation’and‘Keeping Record’are the most important criteria for this approach. In addition, ‘.Training’and‘Identification’are also higher priority of criteria. So, we suggested food safety approach method for improvement of these criteria in conclusion based on discussion with specialist in this field.
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부영양화 해역에서 분리된 Vibrio sp. S-1-2 균의 특성과 Cd 축적에 관한 연구
이원재,박영태,김무찬,윤덕현 한국어병학회 1991 한국어병학회지 Vol.4 No.1
부영양화된 해역인 마산만에서 분리된 해양세균중 카드뮴 200ppm에서 내성을 보인 균을 분리배양하고, 미생물학적 성질과 생체 축적량을 방사성 동위원소인 ^(109)CdCl₂을 이용하여 측정한 결과는 다음과 같다. 1. 분리된 카드뮴 내성균은 Gram음성균이며 단간균으로서 운동성이 있고 생리, 생화학 특성과 TCBS 배지에서의 집락 형태로 보아 Vibrio sp.으로 밝혀졌다. 2. 본 균의 다른 중금속에 대한 내성 실험에서 ZnCl₂, PbCl₂, CuCl₂가 25ppm 함유된 배지에서는 서로 비슷한 성장을 보였지만 HgCl₂ 25ppm에서는 성장이 거의 안되었다. 3. 균체내의 카드뮴 축적량은 배지에 포함된 카드뮴의 양과 비례하여 증가하여 75ppm에서 43.2㎎/ℓ로 최고의 축적을 나타내었고, 100ppm에서의 성장이 저해되었지만 균체 축적량이 일정하게 유지되었다. 4. 방사성 동위원소인 ^(109)CdCl₂를 이용하여 25ppm에서 배양한 후, 중금속 축적조건을 dpm값을 통해 조사한 결과는 96시간 배양시 pH 7에 최대값인 2554×10⁴dpm/㎖을 나타냈고, NaCl에서는 36시간 배양시 611×10⁴dpm/㎖로 최고값을 나타내었다. Vibrio sp. S-1-2 was isolated from seawater in the Masan bay and its bacterial characteristics and bioaccumulation of CdCl₂ in the cell were investigated. As the result of microscopic and biochemical test, the S-1-2 strain was identified to Vibrio sp. and this strain can be tolerated even in 200 ppm CdCl₂ media, however its growth was inhibited. In 25 ppm ZnCl₂ media, the growth of Cd resistant Vibrio sp. S-1-2 was promoted at later stage of growth. The growth of Vibrio sp. S-1-2 was inhibited on 25 ppm CuCl₂ and PbCl₂ media and was not able to grow in 25 ppm HgCl₂ media at all. The uptake of cadium in the cells was increased exponentially with increasing concentration of CdCl₂ in media. But the uptake rate of cadmium was suddenly inhibited at 50 ppm CdCl₂ media. Optimal pH and NaCl concentration for bioaccumulation of CdCl₂ were 8-9 and 1-2%, respectively. In the case of pH, maximum dpm value was found at pH 7 after 96 hours culture and in the case of NaCl concentration, it was detected in 2% NaCl media after 36 hours culture.
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