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이상선(Sang-Sun Lee),성창근(Changkeun Sung),배종찬(Jong-Chan Bae),유진영(Jin-Young Yu) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.5
충북 청원군 등 우리나라의 각기 다른 16곳으로부터 메주를 채취하여 53개의 균을 분리하였다. 이들 다양한 균으로부터 단독균을 사용하여 메주를 제조하고 효소활성도를 측정하였으며 간장을 담갔을 때에 관능검사와 색도를 통하여 이들간의 상관관계를 조사하였다. 균에 의한 간장의 색깔은 다양하게 나타났으나 간장의 맛은 간장 공장에서 중요시 여기는 단백질 분해역가와 관련성이 적었다. 즉 조선 전통메주로부터의 맛은 기질에 대한 효소의 작용양상에 따르는 것으로 나타났다. 또는 조선 전통메주의 맛은 조선 전통메주에 있어서는 최소한 여러가지 복합적인 균이 관여함으로 복합적인 균의 작용에 관련되는 것으로 생각된다. Fifty three microbes, mainly fungal genera, were isolated from sixteen Mejus of different region. From those collected isolates, Meju was manufactured and assayed for the activities of amylase and protease. Correlations between sensory evaluation and color measurement were investigated with Kanjang (soy sauce) prepared by each pure inoculation. Color of Kanjang was quite various depending on fungal genera, but the taste was not quite related with the activity of amylase or protease. This fact might mean that taste of Kanjang depended on the complicate mechanistic action of enzyme for the substrate involved in the soybean hydrolysis. Thus, the taste of Kanjang origenated from Korean traditional Meju seems to belong to complex flora of participated fungal genera as well as Bacillus. sp.
한국전통 식품의 원료인 메주와 누룩에서 분리된 접합균에 대한 연구
유기원,성창근,이상선,유진영 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
The fungal isolates of Mucorales, directly collected from Korean traditional raw materials of Nuruk (raw material for Korean rice wine) and Meju (raw material for Korean soysauces), were compared with those of Rhizopus oryzae purchased. The fungal isolates of Rhizopus, Mucor, and Absidia mostly identified as based on the morphological observations, were evaluated with the PCR-polymorphic bands. The PCR-polymorphic bands of the genomic DNA reacted with the primers of OPD series tenmer were various, but showed averaged 4 to 6 in the agarose-electrophoresis. The dissimilarity coefficient (DC) between two isolates were compared by the cluster analysis, dendrogams and polar ordinations. The isolates of R. oryzae known showed several groupings within the lower value of DC and were divided to two groups of amylo-process and other fungi with other purposes. The isolates unidentified were identified by the DC made of this results. Taxonomy of these isolates made by the morphological observations were consistent with those resulted above in most case but not in all aspects. More works were needed with the isolates known for detail informations of Mucorales.