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최창운,양승대,우광선,정위섭,임수정,서용섭,전권수,안순혁,이종두,홍성운,임상무 ( Chang Woon Choi,Seung Dae Yang,Kwang Sun Woo,Wee Sup Chung,Soo Jung Lim,Yong Sup Suh,Kwon Soo Chun,Soon Hyuk Ahn,Jong Doo Lee,Sung Soon Hong,Sang Moo Lim 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.3
Purpose : The aim of this sutdy was to evaluate the feasibility of 3-[131I]Iodo-O-methyl-L-a-methyltyrosine ([131I]OMINT) as an agent for tumor image. Materlals and Methods: After synthesis of 4-O-methyl-L-a-methyltyrosine (OMAMT), OMAMT was labeled with 131I using Iodogen method. In viro cellular uptake study was performed using 9 L gliosarcoma cells at various time points upto 4 hr. The biodistribution (five rats implanted with the 9 L gliosarcoma cells per group) was evaluated at 30 min, 2 hr, 24 hr after iv injection of 3.7 MBq [131I]OMIMT or L-3-[131I]iodo-a-methyltyrosine ([131I]IMT). Gamma camera images were obtained at 30min, 2 hr, and 24 hr. Results : [131I]OMINT uptake was 3.3 times and 2.5 times higher than [131I]IMT uptake at 30 min and 60 min, respectively and same after 2 hr in in vitro sutdy using 9L gliosarcoma cells. Maximum accumulation in tumor occurred at 30 min for both [131IOMINT and [131I]IMT in tumor bearing rats. The tumor uptake of [131I]OMINT was significantly higher than that of [131I]IMT in tumor bearing rats. The tumor uptake of [131I]OMIMT was significantly higher than that of [131I]IMT at early time point studied (3.74 +- 0.48 vs 0.38 +- 0.17% ID/g at 30 min and 2.40 +- 0.17 vs 0.24 +- 0.03% ID/g at 2 hr, respectively, p<0.01). However, the tumor uptake of both radiolabels were not significantly different at 24 hr (0.04 +- 0.01 vs 0.05 +- 0.01% ID/g). Tumor was visualized as early as at 30 min in gamma camera images. Conclusion : These data suggested that [131I]OMIMT might be a useful tumor imaging agent and has more advantage for the tumor imaging compared to [131I]IMT. (korean J NuclMed 1998;32;290-7)
< 51Cr > Cr ( III )-EDTA 착물 합성 및 < 51Cr > Cr ( III )-EDTA 주사후 두경부 방사능 계측에 의한 사구체 여과율 측정
양승대,임상무,전권수,서용섭,윤용기,박현,우광선,정위섭,오옥두,이종두 ( Seung Dae Yang,Sang Moo Lim,Kwon Soo Chun,Yong Sup Suh,Yong Ki Yoon,Hyun Park,Kwang Sun Woo,Wi Sup Chung,Jong Doo Lee,Ok Doo Oh ) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.3
The purpose of this study is to evaluate the clinical application of the no carrier added[Cr] Cr(UI) EDTA complexes, produced at Korea Cancer Center Hospital. The [Cr]Cr(lll) EDTA complexes, useful for measurement of GFR were prepared at room temperature in the presence of bicarbonate catalysts. The radiochemical purity of[Cr]Cr (Iil) EDTA was over 99% by paper electrophoresis. The time activity curves were obtained by counting the blood samples from 5 volunteers and counting the head and neck regions with whole body counter after inject#ion of the Cr EBTA, respectively, After the nonlinear regression, the area under curve was obtained. The plasma clearance of the Cr-EDTA was calculated with injected dose/AUC. The clearance rate calculated with the head and neck countmg data was in good agreement with t,he result from the plasma sample radioactivity at, 1-3 hrs after injection. From this result, the counting of head and neck region and the nonlinear regression by 2-compartment model could be applied for the measurement of the clearance rate. Using MIRD system, the absorbed radiation dose was calculated by residence time x S. The absorbed whole body radiation dose was negligibly small.
123I , 99mTc 사람 비특이 IgG 및 67Ga - Citrate의 실험동물에서 염증병소 섭취율의 비교
오옥두(Ok Doo Awh),임상무(Sang Moo Lim),이종두(Jong Doo Lee),우광선(Kwang Sun Woo),정위섭(Wee Sup Chung),서용섭(Yong Sup Seo) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.1
N/A 123I has ideal half life of 13 hours, suitable 159 keV gamma energy for imaging, and easy labeling methods. In Korea Cancer Center Hospital, 123I has been produced by MC-50 cyclotron. The purpose of this study is looking for good labeling condition of 123I and 99mTc to nonspecific human polyclonal IgG, and comparing these with 67Ga-citrate in the abscess bearing mice. Human polyclonal nonspecific IgG was labeled with 0.2 M phosphate buffer added 123I by chloramine T method. Human polyclonal nonspecific IgG was labeled with 99mTc-gluconate after activation with β-mercaptoethanol. In the abscess bearing mice, the radioactivity in the abscess was higher in 24 hours than 6 hours after injection. In the abscess, 123I nonspecific IgG had higher uptake than 99mTc-IgG or 67Ga-citrate. There was no significant difference in absecess uptake of 123I-IgG among 24, 72, 120 hours abscess age. Further clinical researches with 123I-nonspecific IgG, and other immunoscintigraphies using 123I are expected.
항체의 Cyclic DTPA를 이용한 99mTc 표지시 Polymer 형성과 체내 동태 변화
오옥두(Ok Doo Awh),임상무(Sang Moo Lim),우광선(Kwang Sun Woo),정위섭(Wee Sup Cung) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.2
N/A Technetium-99m labeling method using bifunctional chelat.ing agent cyclicDTPA has been evaluated with human polyclonal nonspecific IgG. IgG was conjugated with cyclic DTPA with various molar ratio. Reduction of Tc was done with Na,S,O, with various molar excesa Labeling efficiency and identification of polymer was confirmed with HPLC using TSK4000 SW column. Polymer was purified with 100 cm Sepharose 6LB column. Cultured 1x10' Staphylococcus aureus were injected into rat thigh 24 hours later labeled 1gG was injected, and in vivo distribution was observed 4 and 24 hours thereafter. Reduction of 99mTc was optimal with the 10000-50000 times molar excess of Na2S2O4, Polymer formation increased with increasing mloar excess of cyclic DTPA to IgG. Three step labeling-labeling DTPA conjugated IgG after reduction of 99mTc-rnade more polymer than two two step labeling-simultaneous mixing DTPA conjugated IgG, 99mTc and Na2S2O4,. Tc b]ood clearance and lower uptake in the abscess and other organa. IgG conjugated with 200 times molar excess of cyclic DTPA showed slower blood clearance with 200 times molar excess of cyclic DTPA showed slower blaod clearance than that of 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 20 times molar excess. In the 99mTc labeling of IgG with cyclic DTPA for the immunoscintigraphy, obtimalllabeling condition should be chosen, and effect of the ' Tc labeled IgG polymer should be considered.
형광 물질 직접 표지를 위한 Poly Lysine 도입 Lym-1 단일사슬 항체의 제조 및 면역반응성 평가
정재호 ( Jae Ho Jung ),최태현 ( Tae Hyun Choi ),우광선 ( Kwang Sun Woo ),정위섭 ( Wee Sup Chung ),강주현 ( Joo Hyun Kang ),정수영 ( Su Young Jeong ),최창운 ( Chang Woon Choi ),임상무 ( Sang Moo Lim ),천기정 ( Gi Jeong Cheon ) 대한핵의학회 2009 핵의학 분자영상 Vol.43 No.5
목적: 작은 크기의 재조합 단일사슬 항체는 빠른 혈중 제거율과 종양의 항체 집적율이 증가되는 등의 장점을 가지고 있다. 반면에 항체의 작은 크기는 방사성 또는 형광물질의 표지를 위한 킬레이터 결합에 중요한 아미노산 그룹의 감소를 의미하기도 한다. 본 연구에서는 단일사슬 lym-1 염기서열 C-말단에 lysine 아미노산 태그를 삽입하여 형광물질의 직접표지 및 그 표지수율 증가를 확인하고자 하였다. 대상 및 방법: 대장균 pET-22b (+) 벡터에 재조합 된 lysine 삽입 단일사슬 lym-1유전자는 대장균 BL21 (DE3)에 형질전환하여 발현하였다. 생산된 lysine lym-1 항체는 Ni-NTA 컬럽과 분자량 컬럼을 사용해 정제하였고. 단백질 전기영동과 western blot을 통해 확인하였다. lysine lym-1 항체에 방사성 동위원소인 I-124, I-125, I-131 과 Tc-99m를 표지하여 그 수율을 확인하였으며 유세포계측기를 사용해 형광물질인 FITC가 직접표지된 라이신 lym-1 항체의 면역반응성을 사람의 버킷 림프종 세포주인 Raji 세포주에서 면역반응성을 확인하였다. 결과: Lysine 도입 단일사슬 lym-1 항체는 두 과정의 정제를 통하여 획득하였으며 그 크기는 약 48 KDa이었고, 방사성동위원소인 I-124, I-125, I-131과 Tc-99m의 표지수율은 각각 >99%, >99%, >95%, >99%로 확인되었다. 유세포계측을 통한 lysine 도입 단일사슬 lym-1항체의 면역반응성은 기존의 단일사슬 lym-1항체와 유사함을 확인하였다. 결론: 재조합 lym-1 항체에 형광물질을 직접 표지하기 위한 lysine 아미노산의 도입은 항체의 면역반응성 감소를 최소화 시키면서 직접표지 수율을 증가시킬 수 있는 유용한 방법임을 확인하였다. Purpose: Small size of recombinant scFv antibody has many advantages such as rapid blood clearances and improved targeting antibodies to tumor region. On the other hand owing to small size, number of amino group is insufficient in conjugation with chelator and fluorescence labeling. This study is to introduce poly lysine tag to the C-terminal end of scFv lym-1 sequence for fluorescence chelator conjugation. Materials and Methods: Poly lysine scFv lym-1 gene, cloned into pET-22b (+) vector, was expressed in E. coli BL21 (DE3) strain. Antibody purification was performed with Ni-NTA column and then size exclusion column chromatography. Expression and purification levels of poly lysine tagged scFv lym-1 antibody were confirmed by western blot analysis. I-124, I-125, I-131 and Tc-99m were used for radiolabeling of purified poly lysine scFv lym-1. Flow cytometry analysis of FITC conjugated poly lysine scFv lym-1 was performed for confirmation of immunoreactivity of human Burkitt`s lymphoma cells. Results: Poly lysine scFv lym-1 antibody was purified through two steps and identified as molecular weight of 48 KDa. Radiolabeling yields of I-124, I-125, I-131 and Tc-99m into poly lysine scFv lym-1 were >99%, >99%, >95% and >99%, respectively. Flow cytometry analysis of poly lysine scFv and scFv lym-1 was showed similar immunoreactivity to human Burkitt`s lymphoma cells. Conclusion: Poly lysine tag was useful for the sufficient number of amino groups to scFv lym-1 antibody for chelator conjugation with minimizing loss of immunoreactivity. (Nucl Med Mol Imaging 2009;43(5):487-494)
갑상선 암 환자에서 131I 치료시 MIRD Schema에 의한 흡수선량의 평가
김장휘(Jang Hee Kim),임상무(Sang Moo Lim),홍성운(Sung Woon Hong),우광선(Kwang Sun Woo),정위섭(Wee Sup Chung),김기섭(Ki Sup Kim) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.1
N/A Medicai Internal Radiation Dose(MIRD) schema was developed for calculating the absorbed dose from the administered radiopharmaceuticals. With the biological distribution data and the physical properties of the radionuclide we can estimate the absorbed dose by the MIRD schema. For the thyroid cancer patients received 131I therapy, the absorbed dose to the bone marrow is the limiting factor to the administered dose, and the duration of admission is determined by the retained activity in the whole body. To monitor the whole body radioactivity, we used Eberline Smart 200 system using ionization chamber as a detector. With the time activity curve of the whole body, total body residence time was obtained. From the ICRP publication 53, the residence times of the source organs, such as kidney, urinary bladder content and stomach, were, used to calculate the absorbed doses of the target organs, such as stomach, red marrow, b1adder wall and remaineder total body. In 8 thyroid cancer patients with 175 mci of 131I adminstered orally, the mean absorbed dose in the blader wall was 375.1 in the stomach 285.1, red marrow 25.4 and total body 22.4 rad respectively. For the monitoring of the large administered activity, this method seemed to be quite useful.
L - 3 [ 123I ] iodo - α - methyltyrosine 합성과 9L Glioma 이식 백서 분포조사
임상무(Sang Moo Lim),이종두(Jong Doo Lee),서용섭(Yong Sup Suh),전권수(Kwon Soo Chun),우광선(Kwang Sun Woo),정위섭(Wee Sup Chung),양승대(Seung Dae Yang),임종석(Jong Seok Lim),박현(Hyon Park),윤용기(Yong Ki Yun) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.1
N/A L-3[123I]iodo-methyltyrosine([123I] IMT) was synthesized by electrophilic radio-iodination using chloramine-T and Iodobead in phosphate buffered solution. And the biodistribution was examined in 9L glioma bearing rats. The radiosynthesis of [123I] IMT with iodobead was simpler and higher in radiochemical yield(88%) than the method using chloramine-T(83%) as radioiodinating reagent. The highest yield was obtained from the reaction using 1 piece of Iodobead, 200μg α- methyltyrosine in 100μl phosphate-buffered solution (pH 5.5) and the reaction was completed in 7min. 24hours after the injection, the biodistribution in 9L glioma transplanted rats revealed the in vivo deiodination, the excretion via kidney, and 3 times higher uptake in the tumor than normal brain. These results suggest the promising clinical use of [123I]IMT in the various ious malignancies.
농양이식백서에서 99mTc , 188Re 직접표지항체의 비교
임수정,최창운,최태현,임상무,우광선,정위섭 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.1
Purpose: We investigated the direct labeling method of antibody with 99mTc and 188Re and examined the stability and function of these labeled compounds in in vitro and in vivo. Materials and Methods: Disulfide bond of nonspecific human IgG was reduced to -SH group by 2-mercaptoethanol. Stannous ion was used to reduce 99mTc and 188Re. The stability of 99mTc-IgG and 188Re-IgG was estimated upto 24 hrs. Biodistribution was evaluated in abscess bearing rats at 4 and 24 hr post-injection of 99mTc or 188Re labeled IgG. Results: The number of -SH group per reduced IgG molecule was 2.34. The labeling yield of 99mTc-IgG and 188Re-IgG were 90% and 95%, respectively. The stability of 99mTc-IgG at 1, 4, 6 and 24 hr was 91%, 83%, 78%, 7% and that of 188Re-IgG, high uptake was found on kidney, blood, stomach and abscess (9.42±0.68, 1.43±0.24, 0.86±0.18, 0.72±0.10 %ID/g, respectively). The uptakes at 24 hr were kidney, abscess, stomach, and blood in descending order. In case of 188Re-IgG, high uptake at 4 hr post injectappeared on kidney, blood, abscess and stomach (3.92±0.62, 1.32±0.08, 0.88±0.01, 0.26±0.06, respectively). The upatkes at 24 hr were kidney, abscess, blood abd stomach in descending order. The abscess to blood uptake ratio of 99mTc-IgG was 0.5 at 4 hr and 2.02 at 24 hr and that of 188Re-IgG was 0.67 and 1.29. Conclusion: 99mTc-IgG and 188Re-IgG and 188Re-IgG canbe labeled efficiently with direct labeling method. However, 99mTc-IgG and 188Re-IgG, labeled with direct method, was unstable. Further study in needed to enhance the stability of the antibody labeling.