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연지현(Ji-Hyeon Yeon),서현범(Hyeon-Beom Seo),오성호(Sung-Ho Oh),최원석(Won-Seok Choi),강도형(Do Hyung Kang),이현용(Hyeon-Yong Lee),정경환(Kyung-Hwan Jung) 한국생물공학회 2010 KSBB Journal Vol.25 No.3
We investigated the feasibility of bioethanol production from hydrolysate of brown seaweed Sargassum sagamianum. Prior to bioethanol production using yeasts, six yeast strains were compared and the best ones in terms of the ethanol production levels were selected. Pichiastipitis ATCC 7126, Pichia stipitis ATCC 58784, and Pichia stipitis ATCC 58376 were superior to others in terms of ethanol production. These yeast strains were used for producing bioethanol by the shaking bottle culture and the fermentor culture. Out of approximately 30 g/L reducing sugar, about 3~6 g/L and 4~7 g/L bioethanol were produced in the bottle culture and the fermentor one, respectively. Furthermore, it was observed that around 12~28 g-bioethanol was produced from 1 kilogram of Sargassum sagamianum. Compared with those previously published, these data were almost three to eight times higher in value.
고압액화공정을 이용한 구멍갈파래의 발효용 알코올 당화수율 증진
한재건(Jae Gun Han),오성호(Sung Ho Oh),최운용(Woon Yong Choi),권정웅(Kwon Jung Woong),서현범(Hyeon Beom Seo),정경환(Kyung Hwan Jeong),강도형(Do Hyung Kang),이현용(Hyeon Yong Lee) 한국생물공학회 2010 KSBB Journal Vol.25 No.4
Green alga, Ulva pertusa kjelmann has been known to be one of the largest pollutants in Korea. Therefore, the efficient pretreatment processes have been required to improve the yields of fermentable sugar. The optimal pretreatment conditions were determined to be 195℃ for 15 min. The sugar yield of glucose and xylose were estimated as 20.5%, and 5.0% respectively, based on theoretical yields. However solid residues were estimated enzymatic digestibility of 90-95% with cellulase loading of 15 FPU/g glucan. This process was proved to generate the low concentration of Hydroxy-Methyl-Furfural (51 ppm), which resulted in ethanol production with 95% of the maximum conversion yield from glucose in the culture of Saccharomyces cerevisiae (ATCC, 24858). This study showed that Ulva pertusa kjellmann can be used as a bioetahnol resource using the high temperature liquefaction process.
유채대의 이단 고온 처리에 의한 알콜 발효용 당화물 생산
한재건(Jae-Gun Han),오성호(Sung-Ho Oh),정명훈(Myoung-Hoon Jeong),김승섭(Seung-Seop Kim),서현범(Hyeon-Beom Seo),정경환(Kyung-Hwan Jeong),장영석(Young-Seok Jang),김일철(Il-Cheol Kim),이현용(Hyeon-Yong Lee) 한국생물공학회 2009 KSBB Journal Vol.24 No.5
우리 연구팀은, 농산부산물인 유채대의 알콜 발효용 당 화물 생산 가능성을 연구하였다. 이를 위해 농산부산물인 유채대를 연속적으로 5 mL/min의 속도로 산을 이용하지 않고 증류수만을 이용해 이단 고온 처리 (200℃ and 15 Mpa, 375℃ and 23 Mpa)하였다. 본 전처리 공정을 통한 가수수분해물의 당화물 생성은 최종적으로 자일로스와 글루코스의 경우 25.6 g/L, 5.5 g/L가 생성되었다. 이는 유채대에 존재하는 글루코스와 자일로스의 초기 양 대비 각각 18%와 59%의 전환 수율을 나타낸다. 또한 이 공정은 타 공정들에 비하여 대표발효 저해 산물인 HMF의 생성량이 0.2 ppm으로 극히 낮은 수치를 보였으며, 가수분해물의 에탄올 생산시, 글루코스의 발효를 통한 에탄올 생성 전환수율이 90% 이상으로 높은 생성율을 보였다. 따라서 본 공정을 통해 다른 농산부산물이나 해조류 전체에 응용된다면, 고 수율의 에탄올 생산용 당화물을 생산할 것으로 예상한다. Two-step pretreatment process was investigated to efficiently hydrolyzed rape stems for obtaining fermentable sugars. The process was consisted of two consecutive steps as 200℃ and 15 MPa and 374℃ and 24 MPa with the flow rate of 5 mL/min. Under this condition, 5.5 (g/L) of glucose and 25.6 (g/L) of xylose were obtained from rape stems, showing 18% of glucose yield based on 25% cellulose in the rape stems. It was also found that this process could generate less amounts of toxic residues, such as HMF (Hydroxy- Methyl-Furfural) and other fulfural components during hydrolysis process. It could reaction maintain relatively high ethanol production yield as 90% of theoretical conversion yield from glucose. Therefore, this pretreatment process could be applied to hydrolyze other cellulosic and marine resources such as woods, stem and algae for bioethanol production.