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HBV-trimera 동물모델을 통한 B형 간염 항체의 효능평가에 관한 연구
강영국(Young Kook Kang),장명희(Myeong Hee Jang),김근수(Keun-Soo Kim),오미숙(Mee Sook Oh),김남재(Nam Jae Kim),이병석(Byung Seok Lee),이은나(Eun Na Lee),김성주(Sung Joo Kim),류춘제(Chun Jeih Ryu),홍효정(Hyo Jeong Hong) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.1
Humanized Balb/c mice (termed Trimera mice) conditioned by lethal total body irradiation and bone marrow transplantation from NOD/SCID mice have been described to support efficient engraftment of human tissues. To evaluate the efficacy of potential anti-HBV agents in vivo, we, here, describe the development of a mouse Trimera model for human hepatitis B virus (HBV) infection. HBV viremia was induced by transplantation of ex-vivo HBV-infected human liver fragments under the kidney capsule of Trimera mice (HBV-Trimera). The levels of HBV viremia were determined by measuring serum HBV DNA using polymerase chain reaction (PCR) at 10 days after liver transplantation. In order to evaluate the therapeutic potential of two humanized monoclonal antibodies specific to hepatitis B surface antigens, the HBV-Trimera mice were administrated intraperitoneally with anti-HBs antibody (HzSIII) or anti-preS1 antibody (AP301) at days 14 to 17 post-liver transplantation. Treatment of the HBV-Trimera mice with two anti-HBV humanized monoclonal antibodies (HzSIII, AP301) reduced the viral load in their sera in a dose-dependent manner, suggesting that the humanized antibodies will be useful in the prevention and treatment of HBV infection. These results suggest that the HBV-Trimera mice can be used as an animal models for evaluating therapeutic efficacy of anti-HBV agents.