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한일 주거형태 비교에 의한 국산재 이용 증진 방안 고찰
연정윤,강석구,피덕원 한국가구학회 2013 한국가구학회지 Vol.24 No.1
In this study, a dwelling type of Korea and Japan was compared, and consumption rate of domestic timber and its usage was also investigated. In Korea, dwelling type of apartment which constructed by iron and steel-concrete is main shared, but a detached house which is shared at 60% of dwelling type of Japan is mainly constructed by timber. And the rate of the consumption of domestic wood in Japan is 73%. In Japan, there is an effort to substitute imported wood to domestic timber through the promotion of using of domestic timber. Whereas the needs on using of domestic timber gradually emerged. So, the legal about sustainable use of wood is approved by the National Assembly on May 2012. There is not active movement in the aspect of using of domestic timber yet. These causes to the geographical and environmental characteristics, but industry, policy, and the difference of recognize on domestic timber utilization is thought to do greater influence. Hence, it is recommended to make a portfolio of both balanced domestic timber usage and the change of awareness of people by referring to the example of Japan.
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Crystal Structure of a Highly Thermostable α-Carbonic Anhydrase from Persephonella marina EX-H1
김수빈,성종민,연정윤,최승훈,진미선 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.6
Bacterial α-type carbonic anhydrase (α-CA) is a zinc metalloenzyme that catalyzes the reversible and extremely rapid interconversion of carbon dioxide to bicarbonate. In this study, we report the first crystal structure of a hyperthermostable α-CA from Persephonella marina EX-H1 (pmCA) in the absence and presence of competitive inhibitor, acetazolamide. The structure reveals a compactly folded pmCA homodimer in which each monomer consists of a 10-stranded β-sheet in the center. The catalytic zinc ion is coordinated by three highly conserved histidine residues with an exchangeable fourth ligand (a water molecule, a bicarbonate anion, or the sulfonamide group of acetazolamide). Together with an intramolecular disulfide bond, extensive interfacial networks of hydrogen bonds, ionic and hydrophobic interactions stabilize the dimeric structure and are likely responsible for the high thermal stability. We also identified novel binding sites for calcium ions at the crystallographic interface, which serve as molecular glue linking negatively charged and otherwise repulsive surfaces. Furthermore, this large negatively charged patch appears to further increase the thermostability at alkaline pH range via favorable charge-charge interactions between pmCA and solvent molecules. These findings may assist development of novel α-CAs with improved thermal and/or alkaline stability for applications such as CO2 capture and sequestration.
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