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보문 : 느타리속 버섯 계통의 분자생물학적 유연관계의 비교연구
조해진 ( Hae Jin Cho ),이재성 ( Jae Seong Lee ),윤기남 ( Ki Nam Yoon ),( Nuhu Alam ),이경림 ( Kyung Lim Lee ),심미자 ( Mi Ja Shim ),이민웅 ( Min Woong Lee ),정종천 ( Jong Chun Cheong ),신평균 ( Pyung Gyun Shin ),유영복 ( Young 한국균학회 2010 韓國菌學會誌 Vol.38 No.2
Pleurotus spp. have been used for edible and medicinal purposes in Asian countries for a long time. The fruiting bodies of the Pleurotus ostreatus, Pleurotus citrinopileatus and Pleurotus salmoneostramineus contained many physiologically beneficial substances for human health. Therefore, it is necessary to study the genetic diversity of Pleurotus mushroom cultivars commercially cultivated in Korea. Eleven strains of Pleurotus spp. were collected from different geographical regions in South-East Asia and ITS regions of rDNA and RAPD of genomic DNA were analyzed. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 167 to 254 bp and 156 to 213 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into 4 clusters. Eleven Pleurotus species were also analyzed by RAPD with 20 arbitrary primers. Ten of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.1 to 2.0kb. The results revealed that genetic diversity of selected strains of P. ostreatus, P. citrinopileatus and P. salmoneostramineus is low.