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BC3H-1 분화세포에서의 (Na,K)ATPase alpha2 isoform의 표현증대
이경림(Kyung Lim Lee) 대한약학회 1996 약학회지 Vol.40 No.6
The development of the alpha2 isoform of (Na,K)ATPase which is high affinity ouabain receptors was studied in the differentiating nonfusing muscle cell line BC3H-1. The differentiation process of BC3H-1 cell line was confirmed by 2-dexy-D-[3H] glucose uptake experiment and the quantity of the expression of alpha2 isoform was measured using a whole cell [3H] ouabain-binding assay. Undifferentiated growing BC3H-1 cells, myoblasts, exhibited low levels of insulin-stimulated glucose uptake and [3H] ouabain-binding sites. In contrast, differentiated BC3H-1 cells, myocytes, had a 5.6-fold increase in insulin-stimulated glucose uptake and 5-fold increase in [3H] ouabain-binding sites. Scatchard analysis showed that myocytes developed more [3H] ouabain-binding sites than myoblasts vath a dissociation constant (kd) of 6X1O-8M and capacity of 6.lX1O5 sites/cell. Therefore. it seems that myoblasts express low levels of alpha2 subunit and probably the majority of alpha1 subunit, whereas myocytes express high levels of alpha2 isoform. The results indicate that the expression of alpha2 isoform is developmentally regulated during differentiation and that BC3H-1 culture system provides an excellent model for the study of differentiation and mechanism of (Na,K)ATPase action in muscle which requires electrical excitability.
스캠퍼 기법에 따른 여성복 디자인 경향 - 2010 S/S~2020 S/S 패션컬렉션을 중심으로 -
이경림 ( Kyung-lim Lee ) 복식문화학회 2021 服飾文化硏究 Vol.29 No.1
The purpose of this study is to provide basic data to assist students and industrial designers in fashion by examining the trend of designing women’s wear with the SCAMPER method. In the research, the seven types of SCAMPER methods for fashion design were classified based on the previous studies. From 2010 S/S to 2020 S/S, data from 5,149 photographs were collected through overlapping checks and classified by SCAMPER method type. Data analysis was performed using SPSS 25.0 for frequency analysis. As a result, in the SCAMPER “combine” method, more than two items were combined, or structural details were combined with items. In the most applied “adapt” method was involved imitating similar images, or natural forms, or other objects. The “modify” method was applied by changing the shape of some details in basic fashion items. The “magnify” method was applied by enlarging, elongating, or elevating some details of fashion items. The “minify” method was applied by minimizing, shortening, or lowering some details of basic fashion. The method of “put to other use” was expressed throughout the clothing by using non-fabric or trimmings such as metal, beads, and strings. The “rearrange” method was applied by repositioning the top and bottom, front and back, or outside and inside in fashion items and in details. The “reverse” method was applied by reversing the style of fashion, mix-and-match fabric, or switching the gender of the fashion items.
한국산 고등균류의 성분 연구 (제42보) : 이끼살이애기버섯 및 검정나팔버섯의 항암성분
이경림(Kyung Lim Lee),김혜령(Hye Ryung Kim),이정옥(Chong Ock Lee),정경수(Kyung Soo Chung),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1982 생약학회지 Vol.13 No.4
The carpophores of Xeromphalina campanella and Craterellus cornucopioides which were collected at the Gwang-neung area, Gyeong-Gi Province, in 1981 were studied for their antitumor constituents. The carpophores were extracted with not water and alconol precipitates were obtained. These precipitates were brownish powder which were used as samples in antitumor test. The antitumor activity of each sample was tested against sarcoma 180 implanted subcutaueously in ICR mice. The sample obtained from the carpophores of X. campanella showed the inhibition ratios of 47% and 58% at the dose levels of 20㎎ and 50㎎/㎏/day, respectively. The sample obtained from the carpophores of C. cornucopioides showed no activity. The chemical analysis of the antitumor fraction of X. campanella showed that it contained a polysaccharide. The hydrolysates of the polysaccharide moieties contained five monosaccharides: glucose, galactose, mannose, fucose and xylose.
보문 : PCR 다형성 분석에 의한 한국산 잣버섯의 유전적 다양성 및 유연과계
이재성 ( Jae Seong Lee ),조해진 ( Hae Jin Cho ),윤기남 ( Ki Nam Yoon ),( Nuhu Alam ),이경림 ( Kyung Lim Lee ),심미자 ( Mi Ja Shim ),이민웅 ( Min Woong Lee ),이윤혜 ( Yun Hae Lee ),장명준 ( Myoung Jun Jang ),주영철 ( Young Chul 한국균학회 2010 韓國菌學會誌 Vol.38 No.2
Lentinus lepideus, known as train wrecker fungus, has been used for nutritional and medicinal purposes. Recently, commercial cultivation technique and a new cultivar of the mushroom were developed. To investigate the genetic diversity and phylogenetic relationship for identifying the mushroom strains and cultivar, one commercial and 13 strains of Lentinus lepideus from different geographical regions of Korea were analyzed by ITS regions of rDNA and RAPD of genomic DNA. Three strains of Lentinus edodes were also used for the analysis. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 173 to 179 bp and 203 to 205 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical with 156 base pairs. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into four clusters, while 3 strains of L. edodes was divided into a new cluster. Ten primers out of 20 arbitrary primers used in the RAPD-PCR efficiently amplified the genomic DNA. The numbers of amplified DNA bands varied with the primers and strains, with polymorphic DNA fragments in the range from 0.2 to 2.6 kb. The results showed that phylogenetic relationship among Korean strains of Lentnus lepideus is high, but genetic diversity is low.