Limnoperna fortunei의 초기발생 및 유생에 관한 연구

최신석,신창남,Choi, Shin Sok,Shin, Chang Nam 한국패류학회 1985 The Korean Journal of Malacology Vol.1 No.1

The maturity of gonads, early development of the fertilized egg, spawning period, and morphogenesis of larvae in Limnoperua fortunei were studied from October, 1981 to November, 1982 as on attempt to identify its life cycle. 1) Active motility of its sperm was observed at late May, and matured egg could be found at late June. 2) It was estimated that the spawning was occured from late August. This species was belong to the short-term breeder because it finished its spawning within 20 days. 3) It has free-living trochophore and D-shaped larva stage. The shell lengths of early, middle, and D-larval stage were $140.0{\mu}m$, $167.6{\mu}m$ and $210.0{\mu}m$, respectively. The shell heigths of each stage were $97.3{\mu}m$, $137.6{\mu}m$ and $178.2{\mu}m$, respectively.

중석광산지역의 (重石鑛山地域) 수질과 (水質) plankton 상에 관한 연구

최신석,신창남 ( Shin Sok Choi,Chang Nam Shin ) 한국하천호수학회 1976 생태와 환경 Vol.9 No.1·2

Chemical conditions and plankton fauna in the polluted and clear waters were comparatively investigated at the upper stream of the south Han-river situated in Yung-wol, Kangwon-Do during the period of August 1 and August 30, 1969. The results obtained are as follows: 1. Water temperature, pH, NH₃,-N, NO₂-N, NO₃,-N, PO₄^(---), Cl^- was found to be similar both in the polluted and clear waters. And the contents of dissolved oxygen in both waters were statistically very significant. 2. In the polluted water 11 species were appeared especially two species among them were clearly dominant: Cymbella tumida and Filinia longiseta. The frequent appearance of the two species was statistically very significant. 3. In the clear water were found various plankton ranging from 25 to 28 species, especially Gyrosigma accuminata and Ceratium hirundinella were dominant. The frequent appearance of these two species was statistically less significant. Plankton at the confluence of the waters were similar to those in the clear water. 4. Plankton fauna in the polluted and clear waters was affected by the dissolved oxygen called limiting factor which had resulted from the polluted water of tungsten mine.

Species diversity of planktonic crustacean community in Asan Bay

Choi, Shin-Sok,Shin, Chang-Nam,Oh, Kyung-Hwan 충남대학교 자연과학연구소 1979 學術硏究誌 Vol.6 No.1

아산만의 플랭크톤성 갑각류 군집을 대상으로 종의 조성을 조사하여 종다양성을 계산, 분석하고 군집의 종내 및 종간 경쟁과 종구성과의 관련성을 추구하였다. 아산만 플랭크톤성 갑각류 군집의 출현종수는 30종이었으며, 이 군집에서는 종다양도, 종간경쟁이 증가하는 반면에 종내 경쟁은 감소하고 안정도는 낮았다.

Limnoperna fortunei의 초기발생 및 유생에 관한 연구

최신석,신창남 忠南大學校 環境問題硏究所 1983 環境硏究 Vol.1 No.1

민물담치(Limnoperna fortunei)의 생활사를 규명할 목적으로 1981년 10월에서 1982년 11월에 이르기까지 생식소의 성숙 상황, 수정난의 초기 발생과정, 산란시기, 유생의 형태변화에 대하여 조사 연구를 실시하였다. 1. 민물담치는 체외 수정을 하는 자웅이체의 담수 이매패로서, 정자의 활발한 운동성은 5월말에 관찰할 수 있었고, 성숙한 난은 6월말에 관찰되었다. 2. 본종의 산란시기는 7월말에서 8월말까지로 추청되며, 산란기간은 15일∼20일로 short-term breeder에 속하는 종이다. 3. 본종의 유생은 자유 유영하는 trochophore의 유생시기를 가지며, 유생의 성장은 초기에 각장이 140.9㎛, 각고가 97.3㎛이고, 중기에 각장이 167.6㎛, 각고가 137.6㎛이며, 후기에 각장이 210.0㎛ 각고가 178.2㎛이었다. The maturity of gonads, early development of the fertilized egg, spawning period, and morphogenesis of larvae in Limnoperna fortunei were studied from October, 1981 to November, 1982 as on attempt to identify its life cycle. 1. Active motility of its sperm was observed at late May, and matured egg could be found at late June. 2. It was estimated that the spawning was occured from late July to late August. This species was belong to the short-term breeder because it finished its spawning within 20 days. 3. It has free-living trochophore and D-shaped larva stage. The shell lengths of early, middle, and late D-larval stage were 140.0μ, 167.6μ and 210.0μ, respectively. The shell heigths of each stage were 97.3μ, 137.6μ and 178.2μ, respectively.

大蛤과 동죽의 稚貝着生의 土性에 關한 硏究

崔信錫,辛昌男 충남대학교 자연과학연구소 1977 學術硏究誌 Vol.4 No.1

The investigation of young clam beds of Meretrix lusoria and Mactra veneriformis was conducted from July to August 1969, 1974 and 1975 in tidal flat area of Inchon. 1. The result of the analyses of the soil texture of young beds of Meretrix lusoria and Mactra veneriformis was as follows. Meretrix Zusoria: 1%∼2%, silt 30%∼40%, fine sand 60%∼70%, coarse sand 1.5%∼4%. Mactra veneriformis: clay 0.5%∼3%., siit 15%∼70 %, fine sand 50%∼90%, coarse sand 0.1%∼2%. 2. The habitat of young clams of Mactra veneriformis was larger than Meretrix lusoria in the area. The young clams of Meretrix lusoria set in the Lehm soil, and Mactra veneriformis the Lehm and Schuffiger soil contained a lot of sand. 3. The habitat density of young clams of Meretrix lusoria was lower than of Mactra veneriformis. This fact had statistical significance (p<0.05).

海水汚染 指標種으로서의 Heminerita japonica에 關하여

崔信錫,辛昌男,吳炅煥 충남대학교 자연과학연구소 1980 學術硏究誌 Vol.7 No.2

Gastropoda communities were examined to determine the indicators as sea-water pollution in the tidal zone of Inchon and Yongyoudo. Among the factors of sea-water qualities, BOD was 22.42±8.77 ppm and showed higher values than the limiting values of pollution, 5-10 ppm in Inchon Individual number of Heminerita japonica was negatively correlated to the values of BOD.

錦江 淡水二枚貝의 棲息地 土性에 關한 硏究

崔信錫,辛昌男 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.1

The attempt to investigate the relationship between the soil texture of habitat and fresh-water clam was conducted from March to November 1980 in eight stations of Geum River. 1. Unio douglusial, Inversidens reiniana and corbicula Huminea were mainly distributed in the mid-down stream and Anodonta woodiano fukudai, Lanceolaria acrorhyncha and Lamprotula gottschei in the upstream, but Solenaia triangulanis was overall found in abundance. 2. The soil types of fresh-water clam nabitat were divided into three types by analyzing the soil texture; sand (st 1,2,7,8), loamy sand(st 3,4) and sandy loam(st 5,6). 3. The correlation analysis of soil texture and fresh-water clams was significant in Anodonta woodiana fukudai, Inversidens reiniana and corbicula fluminea, but 4 species were not.

Monochrysis lutheri에 對한 無機養料의 影響

崔信錫,辛昌男 충남대학교 자연과학연구소 1976 學術硏究誌 Vol.3 No.2

In this study we investigated the effect by the lack of inorganic minerals on Monochrysis lutheri, grown in the Hirano solution, from June to July 19 76. The numbers in Monochrysis lutheri appeared to delay in increase by the 5th day and get to maximum population around 15th day in Hirano solution. When nirogen, phosphorus and iron were taken up respectively from Hirano solution, the growth rate a s well as the saturation values of the individual namber abated. The differences between the numbers in control Hirano solution and those under the above mentioned conditions were of statistical significance except for the case when silicon was deficient in Hirano soution. The survey on concentration of nitrogen showed that the numbers increased significantly at more than 1.6ml, but phosphorus showed no difference due to its concentration.

病原性 原生動物類의 種鑑別을 爲한 螢光抗體法의 應用

鄭坪林,崔信錫,辛昌男 충남대학교 자연과학연구소 1977 學術硏究誌 Vol.4 No.1

原生動物門中 鞭謀蟲類인 Trichomonas vaginalis, Trichomonas muris, Trichomonas tenax, Trypanosoma cruzi 根足蟲類인 Entamoeba histolyca의 local strain (Ys-27)과 axenic strain (HK-9), Entamoeba muris, Acanthamoeba sp. 그리고 胞子蟲類인 Toxop lasma gondii의 RH 및 Beverley strain을 對象으로 하였다. 上記 原生動物類를 種別로 家免에 注入하여 얻은 亢血淸으로 間接螢光抗體法과 制動反應을 竝行하여 얻은 種別 抗原特異性을 比較한 結果 다음과 같은 結論을 얻었다. (1) Rabbit Anti-Trichomonas vaginalis servum에 對한 Trichomonas vaginalis의 抗原抗體反應에서 最大抗體價는 1:256으로 陽性이었으며, Trichomonas muris와 Trypanosoma cruzi의 境遇, 最大抗體價가 各各 1:4, 1:8에서 交又反應이 일어났고 Trichmonas tenax는 陰性이었다. Rabbit Anti-Trichomonas cruzi serum에서는 Trypanosoma cruzi만이 最大抗體價가 1:128로 陰性이었으며 其他 3種의 Trichomonas는 모두 陰性을 나타냈다. (2) Entameba histolytica(Ys-27)의 抗血淸에 對한 Entamoeba histolytica (Ys-27)의 抗原의 抗原抗體反應은 1:256까지 陰性이었으며, Acanthamoeba sp. 는 1:4에서 交又反應이 있어났으나 無視할 程度이고, Entamoeba muris의 境遇는 陰性이었다. Entamoeba histolytica (HK-9)의 抗血淸에서 Entamoeba histolytica (Ys-27)은 1:32까지 交又反應이 일어났고, Entamoeba muris, Acanthamoeba sp. 는 陰性이었다. (3) Toxoplasma gondii의 Rh strain 抗血淸에 對하여 Rh-strain 自體의 反應에서 1:1024의 最大抗體價를 보였으며, Beverley strain의 抗血淸에 對한 最大抗體價는 1:64로 比較的 높은 交又反應이 일어났다. (4) 根足蟲類와 鞭毛蟲類의 制動反應에서 Entamoeba histolytica (Ys-27)의 抗血淸에 對한 Entamoeba histolytica (Ys-27)의 制動率은 38%, Entamoeba histolytica (HK-9)의 抗血淸에서는 50%으로 모두 陽性이었으며, Trichomonas vaginalis 抗血淸에 對한 Trichomonas vaginalis, Trichomonas tenax의 制動率은 各各 90%, 70%이였고, Trypanosoma cruzi는 45%로 陰性反應으로 看做되었으나 Trypanosoma cruzi 抗血淸에 對한 Trypanosoma cruzi 自體의 反應에서는 90%로 陽性이었고 그 外는 모두 陰性이었다. 以上의 成績으로 보아 間接螢光抗體法은 病原性 原生動物의 抗原特異性을 識別하는데 좋은 長點을 가지고 있었으며 制動反應도 運動性 原生動物類 識別에 一次的 方法으로 推薦되어야 할 것이다. Antigenic comparison of several pathogenic protozoans belonging to class Flagellata, class Rhizopoda (Sarcordina) and class Sporozoa was carried out indirect fluorescent antibody test (IFAT) and immobilization reaction (IR). It was possible for IFAT technique to classify antigenic' specificity between several piotczoan species in the same class except the strains of Toxoplasma gondii, so that this technique is recommendable for further deteailed taxonomical approach. It was also regarded as the best subsidiary immunological tool for the antigenic differentiation of most protozoans with motility.

水質汚染 指標種으로서의 淡水藻類에 關한 硏究

朴鐘聲,崔信錫,申昌男,琴鍾和 忠南大學校 環境問題硏究所 1984 環境硏究 Vol.2 No.2

In order to determine the fresh- water algae as indicators of water pollution, an attempt was conducted from February 28 to October 20, 1984 at the six stations of the Geum River. 1. The St. 1 and 2 showed farirly good water quality. The value of BOD at St. 3 was 6.4 ppm exceeding the permitted level of pollution (5 ppm). Those values at St. 4, 5, and 6 were 2.0- 5.0ppm below the permitted level. 2. DO, BOD, COD, C1-,and NH₃-N contents were significantly different from one another of the surveyed stations of the Geum River. 3. The algae collected at all surveyed stations were of 52 genera 119 species. The relative abundance of Nostoc planctonicum and Oscillatoria limosa were very high at St. 3 where the level of pollution was relatively high and at St. 6 where the water quality was affected by the sea-water under the tidal condition. 4. In the Geum River, Gomphonema olivaceum could be regarded as the most important indicator of the water pollution. The permitted levels of BOD, COD, C1-, and NH₃-N contents for its survival are 5.0, 7.9, 39.1, and 4.1 ppm, respectively.